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SNP markers affecting pig growth traits

A technology of marking and total length, applied in the field of SNP marking

Active Publication Date: 2022-04-26
JIANGXI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And every 1cm increase in body length will increase nearly 1kg of body weight, which will directly increase economic benefits by nearly 10 billion

Method used

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  • SNP markers affecting pig growth traits
  • SNP markers affecting pig growth traits
  • SNP markers affecting pig growth traits

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] 1. Gene filling method (imputation) analysis

[0068] Using the genotype data of 500 individuals for the first-generation sequencing in our laboratory and the resequencing data of 561 individuals downloaded from the National Center for Biotechnology Information (NCBI), use Beagle (4.1) software on the 17th The body length QTL (quantitative traitlocus) region of chromosome 14.5-16.6Mb was used to construct the haplotypes affecting the body length of durometers, so as to obtain all the SNP genotypes in the QTL region. According to Beagle (4.1) software calculation, 56216 SNPs genotypes were finally obtained. Among them, there were 4643 SNPs with genotype accuracy greater than 0.9.

[0069] 2. Data and individual quality control

[0070] Use PLINK v1.07 to perform quality control on the 4643 SNPs genotype data obtained in Section 1 and the 80 genotype data of 500 individuals sequenced in our laboratory: the genotype detection rate <97% or minor alleles were eliminated S...

Embodiment 2

[0108] In this embodiment, the 9 loci that are significantly associated with the relevant growth traits of pigs obtained in Example 1 are subjected to an expanded population validation analysis to determine whether the 9 SNPs loci that affect the relevant growth traits are also the same in a larger population. Effective, that is, expanded to a total of 1515 Du long pig populations (including 500 individuals in Example 1) for verification. Among them, 1515 individuals came from groups slaughtered in different periods and from different pig farms.

[0109] The 9 SNPs loci of each individual in 1515 Du Dachang pigs were sequenced, and the regional association analysis was performed between the 9 SNPs and the carcass length of 1515 Du Da Da pigs through the PLINK v1.07 software, that is, the preparation containing A map file for genotype locations, a ped file for genotype types, and a txt file with phenotypes. The program code is --file file, --pheno phenotype file, --all-pheno, ...

Embodiment 3

[0115] The purpose of this example is to evaluate the correlation between the 9 SNP markers in Example 2 and the growth traits of Landrace and Large White purebred pigs, so as to analyze the purebred breeds from which favorable alleles come from, and to provide a theory for molecular breeding of growth traits and technical support.

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Abstract

The present invention provides SNP markers affecting pig growth traits. The SNP markers include at least one of the following SNP markers: the sequence of the nucleic acid containing the SNP markers is shown in SEQ ID Nos.1 to 9, and the SNP markers are respectively located at The 301st position, followed by T or C, T or G, G or C, G or A, A or G, G or A, A or C, T or C, T or C.

Description

technical field [0001] The invention provides SNP markers that affect growth traits of pigs, especially SNP markers that affect growth traits such as straight carcass length and slanted carcass length of pigs. Background technique [0002] my country is a big agricultural country, and animal husbandry is an important pillar of agriculture, and pigs are the first of the six livestock. Therefore, breeding pig breeds in China can not only increase the economic benefits of enterprises but also stabilize people's livelihood. Growth traits are important indicators of pig economic traits, which mainly include pig carcass straight length, carcass oblique length, carcass weight, etc. Growth traits are mainly affected by genetics. Many studies have found QTL (quantitative trait locus) for pig growth traits, but no causal mutation sites that affect carcass length, oblique length, and body weight have been found. There are few studies on the genetic analysis of growth traits of Du lon...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124
Inventor 黄路生麻骏武李景杨斌郭源梅肖石军
Owner JIANGXI AGRICULTURAL UNIVERSITY
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