Application of polypeptide in preparation of medicine for treating endometriosis

A technology for endometriosis and endometriosis, applied in the field of biomedicine, can solve problems such as large side effects, achieve the effect of relieving abdominal pain and improving endometriosis

Active Publication Date: 2021-08-13
HEILONGJIANG UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] (2) Danazol is a derivative of androgen in pseudomenopausal therapy, which has better effect, but has relatively large side effects

Method used

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  • Application of polypeptide in preparation of medicine for treating endometriosis

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Establishment of animal models of endometriosis

[0022] In the study of endometriosis, the acquisition of endometriosis tissue from clinical patients is limited, so it is necessary to establish animal models of endometriosis. The inventor selected 45 mature female SD rats of SPF grade to establish endometriosis models, and it was identified that 30 SD rats were successfully modeled, with a success rate of 67.7%. Concrete animal experiment steps (referring to CN102988401A) are as follows:

[0023] (1) 45 sexually mature SPF-level healthy unmated female SD rats were obtained from the Animal Center of the First Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine;

[0024] (2) 1% pentobarbital sodium (40mg / kg) intraperitoneal injection anesthesia;

[0025] (3) After routine disinfection, take the middle of the rat's lower abdomen and 1 cm above the pubic symphysis to make a longitudinal incision of about 3 cm;

[0026] (4) Find the uterus on t...

Embodiment 2

[0040] Examining the therapeutic effect of peptides on endometriosis

[0041] Drugs used:

[0042] Gestrinone: Gestrinone capsules produced by Beijing Zizhu Pharmaceutical Company, dissolved in normal saline. The polypeptide whose amino acid sequence is SEQ ID NO.1: was synthesized and sequenced by Shanghai Sangon, and the polypeptide was diluted to 10 μg / kg with physiological saline.

[0043] Experimental steps:

[0044] 32 SD rats successfully modeled were randomly divided into 3 groups, 10 in each group, i.e. 10 negative control groups (physiological saline infusion group), positive control group (gestrinone infusion group, daily 0.5mg / kg) and 10 rats in the polypeptide injection group (10ml / kg per day). The volume of normal saline used in the control group was the same as that used in the polypeptide injection group for 4 consecutive weeks.

[0045] Four weeks later, another laparotomy was performed, and the ectopic endometrium tissue of the corresponding part was taken,...

Embodiment 3

[0055] Embodiment 3 clinical trials

[0056] 1 information

[0057] 1.1 General Information

[0058] Select 40 patients who visited the First Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine from October 2019 to December 2019, and divide them into two groups by random number table method, namely the treatment group and the control group, with 20 patients in each group. There was no significant difference in gender, age, course of disease, etc. between the two groups before treatment, and the balance between the groups was good and comparable.

[0059] 1.2 Diagnostic criteria

[0060] According to the standards stipulated by the third academic meeting of the Obstetrics and Gynecology Professional Committee of the Chinese Association of Integrative Medicine and the "Guiding Principles for Clinical Research of New Chinese Medicines for the Treatment of EM", the following diagnostic criteria are drawn up:

[0061] ① Progressive dysmenorrhea;

[00...

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Abstract

The invention relates to application of polypeptide in preparation of a medicine for treating endometriosis. The polypeptide has an amino acid sequence as shown in SEQ ID NO.1 or an amino acid sequence in which any one amino acid is lacked and changed or one amino acid is added compared with the sequence as shown in SEQ ID NO.1. The polypeptide can improve endometriosis, and is especially suitable for relieving abdominal pain and / or abnormal menstruation caused by the endometriosis.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to the application of polypeptides in the preparation of medicines for treating endometriosis. Background technique [0002] Endometriosis is a common gynecological disease in women that is caused by active endometrial cells growing outside the endometrium. Endometrial cells should grow in the uterine cavity, but because the uterine cavity communicates with the pelvic cavity through the fallopian tubes, endometrial cells can enter the pelvic cavity through the fallopian tubes for ectopic growth. At present, there are many theories about the pathogenesis of this disease, among which the theory of endometrium implantation is generally accepted. The disease mostly occurs in women of childbearing age. It does not develop before puberty, and the ectopic lesions can gradually shrink and degenerate after menopause. The main pathological changes of endometriosis are periodi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/08A61K9/08A61K9/19A61P15/00A61P29/00
CPCA61K38/08A61K9/0019A61K9/08A61K9/19A61P15/00A61P29/00
Inventor 朱小琳韩亚光韩延华贾丽研韩亚鹏
Owner HEILONGJIANG UNIV OF CHINESE MEDICINE
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