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Food additive composition as well as preparation method and application thereof

A technology for food additives and compositions, applied in the field of compositions and their preparations, can solve problems such as unfavorable absorption, unfavorable compositions for human health and the like

Inactive Publication Date: 2021-08-17
山东海之宝海洋科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention aims to solve the technical problem that the existing composition is not conducive to human health and absorption, and provides a food additive composition with anti-oxidation and anti-obesity effects and its preparation method and application

Method used

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  • Food additive composition as well as preparation method and application thereof
  • Food additive composition as well as preparation method and application thereof
  • Food additive composition as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: Vanadium-binding protein and trifuhalol A scavenge DPPH free radical ability

[0021] 400 μL of the combined samples of vanadium-binding protein and Trifuhalol A at different concentrations were mixed with 800 μL of 0.1 mM DPPH ethanol solution, placed in the dark at room temperature for 20 min, and the absorbance was measured at 517 nm.

[0022] Table 1 The combined application of vanadium binding protein and trifuhalol A on DPPH free radical scavenging ability

[0023]

[0024]

[0025] As can be seen from Table 1, the vanadium-binding protein itself does not have the ability to scavenge DPPH free radicals. Experiments have shown that even when the concentration of the vanadium-binding protein reaches 1 mg / mL, it still does not have the ability to scavenge DPPH free radicals; but When it combines with Trifuhalol A, it can significantly enhance the scavenging ability of Trifuhalol A to DPPH free radicals; especially, when the concentration of vanadi...

Embodiment 2

[0026] Embodiment 2: Vanadium-binding protein and Trifuhalol A scavenge ABTS free radical ability

[0027] Mix 7mM ABTS with 2.45mM potassium persulfate 1:1, let stand in the dark at room temperature for 12-16h, dilute to absorbance 0.7±0.02, and set aside. 100 μL of the combined samples of vanadium-binding protein and Trifuhalol A at different concentrations were mixed with 800 μL of 7mM ABTS with an absorbance of 0.7±0.02 and 2.45mM potassium persulfate solution, left to stand in the dark at room temperature for 6min, and the absorbance was measured at 734nm.

[0028] Table 2 The combined application of vanadium binding protein and trifuhalol A on ABTS free radical scavenging ability

[0029]

[0030] It can be seen from Table 2 that both vanadium binding protein and Trifuhalol A have the ability to scavenge ABTS free radicals by themselves, but when the two are combined, the scavenging ability of their composition on ABTS free radicals is significantly enhanced. As the ...

Embodiment 3

[0031] Embodiment 3: vanadium binding protein and Trifuhalol A H 2 o 2 free radical scavenging ability

[0032] 40 μL of the combined samples of vanadium-binding protein and Trifuhalol A at different concentrations were mixed with 100 μL of PBS (pH 5.0), and then 20 μL of 2 mM H 2 o 2, Incubate at 37°C for 5 minutes and set aside. 30 μL 1.25mM ABTS and 30 μL 1U / mL peroxidase were added to the ready-to-use solution, and incubated at 37°C for 10min. Absorbance was measured at 410nM.

[0033] Table 3 Combined application of vanadium binding protein and trifuhalol A on H 2 o 2 free radical scavenging ability

[0034]

[0035]

[0036] It can be seen from Table 3 that both vanadium-binding protein and Trifuhalol A have the ability to H 2 o 2free radical scavenging ability, but when the two are combined, their composition will have an impact on the scavenging ability of ABTS free radicals; The activity of scavenging hydrogen peroxide free radicals of the composition ...

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Abstract

The invention relates to a food additive composition and a preparation method and application thereof, and solves the technical problem that the existing composition is not beneficial to human health and absorption. The food additive composition contains vanadium binding protein and 5-2,6-dihydroxy-4-(2,4,6-trihydroxyphenyl)benzene-1,2,3-trihydroxybenzene. The invention also provides a preparation method and application thereof. The method can be used in the field of preparation of anti-oxidation and anti-obesity food.

Description

technical field [0001] The invention relates to a composition and its preparation method and application, in particular to a food additive composition and its preparation method and application. Background technique [0002] Obesity has become a worldwide public problem affecting human health. Genetic background, living habits and eating habits can all lead to obesity. In addition, obesity can also induce diabetes, cardiovascular disease, hypertension, and hyperlipidemia. As of 2017, there are approximately 450 million diabetics worldwide. [0003] The Chinese invention patent application whose publication number is CN110123843A discloses a composition for reducing weight and its application. 5 parts of the fat-soluble extract of Brachydia zoster; the lipo-soluble extract of Brachydia zodiac is obtained by homogenizing the sea squirt, filtering the filtrate, extracting the filtrate with n-butanol and concentrating it. The composition provided by the patent application ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/17A23L33/10
CPCA23L33/17A23L33/10A23V2002/00A23V2200/30A23V2200/332A23V2250/542A23V2250/202
Inventor 刘晓勇金相武张爽王少茜许玲玉王伶秀肖克
Owner 山东海之宝海洋科技有限公司
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