Tobramycin detection test paper based on aptamer and platinum modified gold nanoparticles

A technology of gold nanoparticles and tobramycin, which is applied in the fields of analytical chemistry, medicine, environment and food testing, can solve problems such as the limitation of visual detection of tobramycin, achieve quantitative analysis, visualize results, and improve detection efficiency. Effect
CN113278684AActive Publication Date: 2021-08-20JIANGNAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
JIANGNAN UNIV
Publication Date
2021-08-20

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Abstract

The invention relates to tobramycin detection test paper based on an aptamer and platinum modified gold nanoparticles. The test paper comprises a sample pad, a gold label pad, a nitrocellulose membrane, a water absorption pad, a PVC base plate, a detection line and a quality control line. Platinum-modified gold nanoparticles (Au@PtNPs) are used for loading a hybrid double strand of an aptamer (Apt) and a complementary probe (cDNA) to be used as a signal amplification label. The unique bifunctional properties (plasma optical property and nano mimic enzyme catalytic property) of Au@Pt NPs provide two different detection schemes: one is red only generated by the intrinsic color of AuNPs, and the other is more sensitive dark blue generated by a catalytic substrate, so that a detection mode adjusted as required is realized. The test paper strip provided by the invention is simple, convenient and rapid in use, is sensitive and efficient, and has huge application potential in field point detection of tobramycin residues in food and environment.
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Description

technical field

[0001] The invention relates to the fields of analytical chemistry, medicine, environment and food detection, in particular to a tobramycin detection test paper based on aptamers and platinum-modified gold nanoparticles. Background technique

[0002] Tobramycin (Tobramycin) is a secondary metabolite produced by Streptomyces oberurus fermentation, molecular formula: C18H37N5O9, molecular weight 467.51, belongs to aminoglycoside antibiotics, has the characteristics of good water solubility, stable properties, broad antibacterial spectrum, etc., in clinical and It is widely used in animal medicine to treat infections caused by microorganisms such as Gram-negative bacteria. However, the excessive use of tobramycin in agriculture, animal husbandry, aquaculture and other fields will lead to its extensive residues in animal-derived foods and ecological environments, and eventually accumulate in the human body through the food chain. This may have a serious impact o...

Claims

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