Method for rapidly analyzing microbiome information in bile of acute cholecystitis patient to realize precise treatment

Abstract

The invention relates to a method for rapidly analyzing microbiome information in bile of an acute cholecystitis patient to realize precise treatment. The method comprises the following steps: step 1, collecting a bile sample; step 2, storing the bile sample; step 3, extracting bile set DNA; and step 4, carrying out bioinformation analysis. According to the method for rapidly analyzing the microbiome information in the bile of the acute cholecystitis patient to achieve precise treatment, 16S rDNA sequencing precise analysis is carried out on a bile sample, the difference of individual infection flora is known, assistance is provided for subsequent precise treatment, and according to unique attributes of bacteria, a more satisfactory treatment effect can be achieved only by selecting more suitable antibiotics. Tthe method is expected to become a standardized treatment scheme for acute cholecystitis perioperative period treatment.

Description

technical field [0001] The invention relates to a method for rapidly analyzing the information of microbial groups in the bile of patients with acute cholecystitis to realize precise treatment. Background technique [0002] Microorganisms are closely related to human health and diseases. Microorganisms living in or on the body interact with the human body and maintain a certain balance. Sometimes surgical infection-related diseases (such as cholecystitis, biliary tract infection, etc.) have surgical contraindications or cannot be operated immediately, and further elective surgical treatment is required after the infection is controlled, and some even adopt non-surgical conservative treatment. In these cases, precise and effective treatment is required. anti-infective treatment regimen. In the past, we used more drugs based on experience, and it was difficult to guarantee the efficacy and therapeutic effect. Bacterial culture, due to factors such as long culture time and har...

AI Technical Summary

Problems solved by technology

Microbial culture technology has problems such as time-consuming, biased, low sensitivity and accuracy, inability to identify non-cultured bacteria, and difficulty in recovering all microorganisms. Moreover, the living conditions of microorganisms are relatively harsh, and the micro-ecosystem simulation in the human body exists. It is more difficult, resulting in a considerable proportion of microorganisms that cannot be obtained in the laboratory

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