Creation method of special soybean protein germplasm for processing

Abstract

The invention discloses a creation method of a special soybean protein germplasm for processing. The method comprises steps as follows: abundant seed main storage protein 7S and 11S component subunit content variation genes in soybean germplasm resources are utilized for carrying out identification and selection by means of pyramiding breeding, crossbreeding and the like in combination with an SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis) method and a molecular identification means, and firstly, germplasms only containing a 7S component and germplasms only containing a 11S component are created; then hybridized combinations with a series of germplasms highly containing the 7S component and a series of germplasms highly containing the 11S components obtained by screening are configured respectively, a series of materials only containing and highly containing the 7S component and a series of materials only containing and highly containing the 11S component are obtained through selection and stabilization, and strains are cultivated; and nutritional characteristics, functionality and processing characteristics are evaluated respectively, and the strains are classified into special soybean materials suitable for different processed products, and therefore, high-quality special raw materials are provided for soybean processing and utilization.

Description

technical field [0001] The invention belongs to the fields of soybean genetic breeding, quality improvement and processing and utilization, and relates to a method for creating a processing-specific soybean protein germplasm. Background technique [0002] Soybean seed protein and oil content account for about 40% and 20% of the dry weight of the seed, respectively. The utilization of soybean seed protein is not only the use of its nutrition, such as it contains 8 kinds of amino acids necessary for the human body, it has the functions of anti-tumor, enhancing immunity, preventing cardiovascular diseases, etc., but more importantly, it uses its processing characteristics-functionality, Such as gelling, emulsifying, foaming, oil holding, water holding, etc. to improve the texture and nutrition of food. The latter is especially important in the food processing industry. For example, meat products need to use the oil-holding and water-holding properties of soybean protein, the c...

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Problems solved by technology

However, using these deletion materials through cross breeding and aggregation breeding, not only the creation of materials containing only 7S components and only 11S components is rarely reported at home and abroad, but also there is no creation of a series containing only and high 7S components. The excellent germplasm and series that have large differences in the content of their constituent subunits (α′, α and β) only contain and highly contain 11S components and their constituent subunits (A 1b B 1b 、A 1b B 2 、A 2 B 1a 、A 3 B 4 、A 5 A 4 B 3 ) reports of excellent germplasm with large differences in the content of each other, etc.

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