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Transferrin receptor targeting peptides

A transferrin and receptor technology, applied in the direction of polypeptides, peptides/protein components, peptides containing localization/targeting motifs, etc., can solve the problems of poor response, difficult to use conventional drugs for diseases, etc.

Pending Publication Date: 2021-08-31
弗莱德哈钦森癌症中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The BBB exists to keep toxic metabolites and pathogens out of the brain, but also plays a role in making diseases of the CNS particularly difficult to treat with conventional pharmaceuticals
For example, primary CNS tumors such as gliomas, as well as neuroinflammatory and neurodegenerative diseases such as multiple sclerosis and Alzheimer's Disease, are critical to similar diseases affecting peripheral tissues. Cases where the response to the more effective treatment modality will be differently particularly poor

Method used

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  • Transferrin receptor targeting peptides
  • Transferrin receptor targeting peptides
  • Transferrin receptor targeting peptides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach

[0616]The following embodiments describe non-limiting permutations of combinations of features disclosed herein. Other permutations of combinations of features are also contemplated. In particular, each of these numbered embodiments shall be considered dependent on or related to each preceding or subsequent numbered embodiment, regardless of their order as listed. CLAIMS 1. A composition comprising an engineered transferrin receptor (TfR) binding peptide or variant, homologue, fragment or analog thereof. 2. A composition comprising a peptide construct, wherein the peptide construct comprises: a) a transferrin receptor (TfR) binding peptide or a variant, homologue, fragment or analog thereof; and b) an active agent, wherein said peptide is conjugated to, linked to or fused to said active agent. 3. The composition of any one of embodiments 1-2, wherein the TfR is human TfR, or murine TfR, or both human TfR and murine TfR. 4. A composition comprising an engineered peptide with...

Embodiment 1

[0620] Peptide Manufacturing

[0621] This example describes the peptides and peptide constructs described herein (e.g., SEQ ID NO: 1 - SEQ ID NO: 70, SEQ ID NO: 136 - SEQ ID NO: 205, SEQ ID NO: 225 - SEQ ID NO: 332, or any of SEQ ID NO:356-SEQ ID NO:361). Published methods were used to produce protein-derived peptides in mammalian cell culture. (A.D.Bandaranayke, C.Correnti, B.Y.Ryu, M.Brault, R.K.Strong, D.Rawlings. 2011. Daedalus: a robust, turnkey platform for rapid production of decigram quantities of active recombinant proteins in human cell lines using novel lentiviral vectors. Nucleic Aci Research. (39) 21, e143).

[0622] The peptide sequence was reverse translated into DNA, synthesized, and cloned in-frame with siderophilin using standard molecular biology techniques (M.R. Green, Joseph Sambrook. Molecular Cloning. 2012 Cold Spring Harbor Press). The resulting construct was packaged into lentivirus, transduced into HEK-293 cells, amplified, isolated by immobilized...

Embodiment 2

[0624] Peptide Expression Using Mammalian Expression Systems

[0625] This example describes the use of mammalian expression systems to express peptides and peptide constructs. Peptides were expressed according to the method described in Bandaranayake et al., Nucleic Acids Res. 2011 Nov;39(21):e143. Tobacco etch virus protease is used to cleave peptides from siderophage (e.g., SEQ ID NO: 1 - SEQ ID NO: 70, SEQ ID NO: 136 - SEQ ID NO: 205, SEQ ID NO: 225 - SEQ ID NO: 332, or Any one of SEQ ID NO:356-SEQ ID NO:361), and by carrying out reverse-phase HPLC (RP-HPLC) with the gradient of acetonitrile and 0.1% TFA aqueous solution to purify, then aliquot and lyophilize for slightly used later. Molecular weights were verified by mass spectrometry.

[0626] To optimize and validate the screening method and to identify TfR-binding peptides, the transferrin receptor (TfR) ectodomain ("soluble TfR", SEQ ID NO:353) was cloned into the Daedalus soluble protein producing lentiviral vecto...

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Abstract

Described herein are peptides and variants thereof as well as peptide constructs comprising peptides conjugated to, linked to, or fused to agents, wherein the peptides and peptide constructs are capable of binding TfR. Binding of a peptide or peptide construct as described herein to TfR can enable transcytosis across an endothelial layer, e.g., the blood brain barrier, or the crossing of a cell membrane. Pharmaceutical compositions and uses of peptides and peptide constructs, as well as methods of designing and manufacturing such peptides and peptide constructs, to treat a disease or condition are also described herein.

Description

[0001] cross reference [0002] This application claims priority and benefit to U.S. Provisional Application No. 62 / 779,885, filed December 14, 2018, and U.S. Provisional Application No. 62 / 836,520, filed April 19, 2019, each of which The entire content of one is hereby incorporated by reference. [0003] sequence listing [0004] This application contains a Sequence Listing that has been filed electronically in ASCII format and is hereby incorporated by reference herein in its entirety. The ASCII copy, created on December 12, 2019, is named 44189-723_601_SL.txt and is 552,571 bytes in size. Background technique [0005] Delivery of drugs to targets in the central nervous system (CNS) is impeded by the blood-brain barrier (BBB), a term used for the particularly impenetrable vascular endothelial cells in CNS capillaries. The BBB exists to keep toxic metabolites and pathogens out of the brain, but also functions to make diseases of the CNS particularly difficult to treat with...

Claims

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Application Information

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IPC IPC(8): A61K38/03A61P43/00A61K49/00C07K7/08C07K9/00
CPCA61P43/00C07K2319/10C07K14/47A61K9/0019A61K47/645A61K49/0056C40B30/04G16B15/20A61K38/00A61P25/28A61P25/04A61P1/00A61P35/00A61K51/088C12N15/1089A61K38/16
Inventor Z·克鲁克M-Y·布鲁斯耐克J·奥尔森A·J·梅瑞尹春风G·G·郝平R·斯特朗
Owner 弗莱德哈钦森癌症中心
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