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Method for separating and breeding pure-color dendrobium flowers

A technology of dendrobium and solid color, which is applied in the field of separation breeding, can solve problems not related to the method of separation and breeding of dendrobium solid color flowers, and achieve the effect of large number of pure color flower plants, high separation rate, and easy rooting

Active Publication Date: 2021-09-03
HAINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Neither of the two patents involves the method for the separation and breeding of pure-colored flowers of Dendrobium orchids, but only involves the technical issues of improving the cultivation efficiency of Dendrobium orchids and cultivating new hybrid varieties

Method used

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  • Method for separating and breeding pure-color dendrobium flowers
  • Method for separating and breeding pure-color dendrobium flowers
  • Method for separating and breeding pure-color dendrobium flowers

Examples

Experimental program
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Effect test

Embodiment 1

[0025] S1 explant selection: use Dendrobium orchid lateral bud stems as explants, rinse with tap water, soak in diluted detergent for 10 minutes, rinse with tap water, soak in 70% alcohol by volume for 60 seconds, then rinse with sterile water for 5 times, Remove the leaves and membranous leaf sheaths on the ultra-clean bench, and transfer to 0.1% HgCl with a mass concentration of 1 drop of Tween-80 2 The solution was disinfected for 8 minutes, rinsed with sterile water for 5 times, and blotted dry with sterile gauze or sterile filter paper.

[0026] S2 protocorm induction: Inoculate the sterilized lateral buds on the induction medium with the formula of 1 / 2MS+BA 1mg / L+NAA0.1mg / L for the first solid-state culture, culture for 45 days, culture in the light for 14 hours every day, and culture in the dark 10h, light culture conditions are light intensity 1500Lux, temperature 26°C, dark culture temperature is 26°C; when the new axillary bud grows to 0.8cm, after 2 leaves, cut off ...

Embodiment 2

[0032] S1 explant selection: use Dendrobium orchid lateral bud stems as explants, rinse with tap water, soak in diluted detergent for 10 minutes, rinse with tap water, soak in 70% alcohol by volume for 60 seconds, then rinse with sterile water for 5 times, Remove the leaves and membranous leaf sheaths on the ultra-clean bench, and transfer to 0.1% HgCl with a mass concentration of 1 drop of Tween-80 2 The solution was disinfected for 8 minutes, rinsed with sterile water for 5 times, and blotted dry with sterile gauze or sterile filter paper.

[0033] S2 protocorm induction: Inoculate the sterilized lateral buds on the induction medium with the formula of 1 / 2MS+BA 4mg / L+NAA0.2mg / L for the first solid-state culture, culture for 45 days, culture in the light for 14 hours every day, and culture in the dark 10h, light culture conditions are light intensity 1500Lux, temperature 26°C, dark culture temperature is 26°C; when the new axillary bud grows to 0.8cm, after 2 leaves, cut off ...

Embodiment 3

[0039] S1 explant selection: use Dendrobium orchid lateral bud stems as explants, rinse with tap water, soak in diluted detergent for 10 minutes, rinse with tap water, soak in 70% alcohol by volume for 60 seconds, then rinse with sterile water for 5 times, Remove the leaves and membranous leaf sheaths on the ultra-clean bench, and transfer to 0.1% HgCl with a mass concentration of 1 drop of Tween-80 2 The solution was disinfected for 8 minutes, rinsed with sterile water for 5 times, and blotted dry with sterile gauze or sterile filter paper.

[0040] S2 protocorm induction: Inoculate the sterilized lateral buds on the induction medium with the formula of 1 / 2MS+BA 3mg / L+NAA0.2mg / L for the first solid-state culture, culture for 45 days, culture in the light for 14 hours every day, and culture in the dark 10h, light culture conditions are light intensity 1500Lux, temperature 26°C, dark culture temperature is 26°C; when the new axillary bud grows to 0.8cm, after 2 leaves, cut off ...

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Abstract

The invention provides a method for separating and breeding pure-color dendrobium flowers. The method comprises the following steps that lateral bud stems of dendrobium are taken as explants, a protocorm induction culture medium is prepared from 3mg / L of 1 / 2MS+BA and 0.2 mg / L of NAA, a protocorm proliferation culture medium is prepared from 3mg / L of 1 / 2MS+BA, 0.2 mg / L of NAA, 30g / L of bananas and 20g / L of potatoes, and by combining liquid suspension culture and a treatment mode of cutting off new buds to separate and breed the pure-color dendrobium flowers. According to the method for separating and breeding the pure-color dendrobium flowers, the protocorm proliferation rate can be improved, stable pure color flowers are effectively separated from the dendrobium nobile flower color chimera, and the separation rate reaches 76.09%.

Description

technical field [0001] The invention relates to the technical field of separation and breeding, in particular to a method for separation and breeding of solid-colored dendrobium orchid flowers. Background technique [0002] Dendrobium orchid, Dendrobium orchidaceae is a perennial herbaceous epiphytic plant, with huge racemes, various flower colors, and gorgeous and colorful. Dendrobium has many flower color chimeras, such as red and white flower color chimeras. The flower color and pattern of such flower color chimeras are unstable during reproduction, and it is difficult to keep consistent with the mother plant. And pure color flower or single color flower varieties can maintain the stability of flower color in the breeding process, and have higher commerciality. [0003] Dendrobium orchid conventional propagation methods are branching, branching and cuttings, but these methods have larger propagules and low reproductive rates. Dendrobium orchid can be rapidly propagated ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 谢光明李秀梅刘进平陈银华
Owner HAINAN UNIVERSITY
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