Preparation method of pre-vascularized biological structure body with multi-scale channel network

Abstract

The invention relates to a preparation method of a pre-vascularized biological structure body with a multi-scale channel network. The preparation method comprises the following specific steps: (1) preparing hydrogel microspheres; (2) preparing a sacrificial model; (3) preparing a biological structure body mold; and (4) preparing the biological structure. According to the method, the hydrogel microspheres are used as a basic construction unit of the biological structure body, and micro gaps between the spheres generated after the microspheres are stacked are combined with a macro-scale three-dimensional vascular network prepared by additive and subtractive composite forming, so that the controllable preparation of the pre-vascularized biological structure body with the three-dimensional multi-scale and high-density vascular network is realized.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and biomanufacturing, and in particular relates to a method for preparing a prevascularized biological structure with a multi-scale channel network. Background technique [0002] Organ transplantation is the golden treatment for patients with end-stage organ failure. Due to the limited number of donors, it is necessary to develop new methods to achieve regeneration and repair of damaged organs. Tissue engineering is a potential method to manufacture damaged tissue and organ substitutes. However, the fabrication of complex organs or clinical-scale tissues still faces great challenges, one of which is the vascularization of tissues. Oxygen transport is a key factor for cell survival, and its diffusion distance in tissues is generally 150-200 μm. Therefore, under physiological cell density conditions, tissues with a thickness greater than 400 μm will produce necrotic cores. In addition, the abse...

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Problems solved by technology

At present, great progress has been made in the controllable preparation of medium-to-large-scale vascular channels with three-dimensional structures by integrating bio-3D printing technology and sacrificial materials by adding and subtracting composite forming methods. However, limited by model design and forming principles, At present, there are still problems of narrow variation range of three-dimensional multi-scale blood vessel diameter and insufficient spatial distribution density of blood vessel network, which cannot support the long-term survival of biological structures with physiological cell density.

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