Method for extracting procyanidine from aronia melanocarpa fruits
A technology of proanthocyanidins and rowan fruit, which is applied in the field of extraction of plant active ingredients, can solve the problems of inability to release proanthocyanidins and low extraction rate, achieve large-scale and industrial production, increase extraction rate, and simple equipment
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Embodiment 1
[0049] see figure 1 , the present embodiment extracts the method for proanthocyanidins from Aronia adenocarpus fruit, and the steps are as follows:
[0050] 1) Crushing and pulping of raw materials
[0051] Mechanically mash the fruit of Aronia adenocarpus into pulp and pass through a 100-mesh sieve to obtain fruit juice and pomace.
[0052] 2) Microbial fermentation of pomace
[0053] Add deionized water to the fermenter, prepare a liquid medium according to 0.2g / L peptone, 2g / L sodium chloride, 10g / L magnesium sulfate and 5g / L dipotassium hydrogen phosphate, and then add the above pomace to 200g / L The dosage is put into the liquid medium.
[0054] Inoculate Aspergillus niger, Trichoderma viride and Bacillus licheniformis into the liquid culture medium added with pomace, the inoculum amounts are 0.5%, 0.2% and 0.2% of the pomace amount respectively; , pressure 0.05MPa, rotation speed 120rpm under the conditions of constant temperature stirring and fermentation for 20h, to...
Embodiment 2
[0061] The present embodiment extracts the method for proanthocyanidins from Aronia adenocarpus fruit, and the steps are as follows:
[0062] 1) Crushing and pulping of raw materials
[0063] Mechanically mash the fruit of Aronia adenocarpus into pulp and pass it through a 200-mesh sieve to obtain fruit juice and pomace.
[0064] 2) Microbial fermentation of pomace
[0065] Add deionized water in the fermenter, prepare liquid culture medium according to 10g / L peptone, 0.2g / L sodium chloride, 0.2g / L magnesium sulfate and 10g / L dipotassium hydrogen phosphate, then the pomace of step 1) Drop into the above-mentioned liquid medium according to the dosage of 500g / L.
[0066] Inoculate Aspergillus niger, Trichoderma viride and Bacillus licheniformis into the liquid culture medium added with pomace, the inoculum amounts are 0.1%, 0.1% and 1% of the pomace amount respectively; , pressure 0.04MPa, rotating speed 100rpm under the conditions of constant temperature stirring and fermen...
Embodiment 3
[0074] The present embodiment extracts the method for proanthocyanidins from Aronia adenocarpus fruit, and the steps are as follows:
[0075] 1) Crushing and pulping of raw materials
[0076] Mechanically mash the fruit of Aronia nigra into pulp and pass through a 120-mesh sieve to obtain fruit juice and pomace.
[0077] 2) Microbial fermentation of pomace
[0078] Add deionized water in the fermenter, prepare liquid culture medium according to 2g / L peptone, 10g / L sodium chloride, 5g / L magnesium sulfate and 0.2g / L dipotassium hydrogen phosphate, then the fruit pomace of step 1) according to The dosage of 300g / L was dropped into the above-mentioned liquid culture medium.
[0079] Inoculate Aspergillus niger, Trichoderma viride and Bacillus licheniformis into the liquid culture medium added with pomace, the inoculum amounts are 0.3%, 0.5% and 0.5% of the pomace amount respectively; at fermentation temperature 32°C, ventilation rate 1:1.1 , pressure 0.06MPa, rotating speed 200...
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