Method for extracting procyanidine from aronia melanocarpa fruits

A technology of proanthocyanidins and rowan fruit, which is applied in the field of extraction of plant active ingredients, can solve the problems of inability to release proanthocyanidins and low extraction rate, achieve large-scale and industrial production, increase extraction rate, and simple equipment

Active Publication Date: 2021-09-03
秦皇岛惠恩生物技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology uses different methods to break down the outer layer (the shell) covering the stone called Punjaponica fruits' necrotic stalk into small pieces that help it be more easily absorbed with nutrients like flavonoids or polyphenols found naturally within them. These tiny fragments make better use than pure peptides alone because they have higher levels of activity compared to their parent compounds themselves due to greater surface area exposed on the inside walls. Additionally, this technique allows for effective separation between valuable components without adding too much chemical agents during the digestion step. Overall, these technical results lead to improved efficiency and economics when producing certain types of food products containing natural ingredients.

Problems solved by technology

This patented technical describes methods for producing procyguannides called probacinoside A and related molecules like quercitrin B and luteolin. These chemicals play important roles in maintaining healthy cell membranes while being beneficial ingredients in many products including drugs and diet supplements. They help improve their quality and functionality through reducing harmful effects on human body cells caused by excessive oxidative stressors. Additionally, these materials may provide valuable resources for research purposes due to their unique properties.

Method used

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  • Method for extracting procyanidine from aronia melanocarpa fruits

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Experimental program
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Embodiment 1

[0049] see figure 1 , the present embodiment extracts the method for proanthocyanidins from Aronia adenocarpus fruit, and the steps are as follows:

[0050] 1) Crushing and pulping of raw materials

[0051] Mechanically mash the fruit of Aronia adenocarpus into pulp and pass through a 100-mesh sieve to obtain fruit juice and pomace.

[0052] 2) Microbial fermentation of pomace

[0053] Add deionized water to the fermenter, prepare a liquid medium according to 0.2g / L peptone, 2g / L sodium chloride, 10g / L magnesium sulfate and 5g / L dipotassium hydrogen phosphate, and then add the above pomace to 200g / L The dosage is put into the liquid medium.

[0054] Inoculate Aspergillus niger, Trichoderma viride and Bacillus licheniformis into the liquid culture medium added with pomace, the inoculum amounts are 0.5%, 0.2% and 0.2% of the pomace amount respectively; , pressure 0.05MPa, rotation speed 120rpm under the conditions of constant temperature stirring and fermentation for 20h, to...

Embodiment 2

[0061] The present embodiment extracts the method for proanthocyanidins from Aronia adenocarpus fruit, and the steps are as follows:

[0062] 1) Crushing and pulping of raw materials

[0063] Mechanically mash the fruit of Aronia adenocarpus into pulp and pass it through a 200-mesh sieve to obtain fruit juice and pomace.

[0064] 2) Microbial fermentation of pomace

[0065] Add deionized water in the fermenter, prepare liquid culture medium according to 10g / L peptone, 0.2g / L sodium chloride, 0.2g / L magnesium sulfate and 10g / L dipotassium hydrogen phosphate, then the pomace of step 1) Drop into the above-mentioned liquid medium according to the dosage of 500g / L.

[0066] Inoculate Aspergillus niger, Trichoderma viride and Bacillus licheniformis into the liquid culture medium added with pomace, the inoculum amounts are 0.1%, 0.1% and 1% of the pomace amount respectively; , pressure 0.04MPa, rotating speed 100rpm under the conditions of constant temperature stirring and fermen...

Embodiment 3

[0074] The present embodiment extracts the method for proanthocyanidins from Aronia adenocarpus fruit, and the steps are as follows:

[0075] 1) Crushing and pulping of raw materials

[0076] Mechanically mash the fruit of Aronia nigra into pulp and pass through a 120-mesh sieve to obtain fruit juice and pomace.

[0077] 2) Microbial fermentation of pomace

[0078] Add deionized water in the fermenter, prepare liquid culture medium according to 2g / L peptone, 10g / L sodium chloride, 5g / L magnesium sulfate and 0.2g / L dipotassium hydrogen phosphate, then the fruit pomace of step 1) according to The dosage of 300g / L was dropped into the above-mentioned liquid culture medium.

[0079] Inoculate Aspergillus niger, Trichoderma viride and Bacillus licheniformis into the liquid culture medium added with pomace, the inoculum amounts are 0.3%, 0.5% and 0.5% of the pomace amount respectively; at fermentation temperature 32°C, ventilation rate 1:1.1 , pressure 0.06MPa, rotating speed 200...

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Abstract

The invention provides a method for extracting procyanidine from aronia melanocarpa fruits. The method comprises the following steps: A) mashing aronia melanocarpa fruits, and filtering to obtain juice and pomace; b) fermenting the pomace by adopting aspergillus niger, trichoderma viride and bacillus licheniformis to obtain pomace fermentation liquor; c) mixing the fruit juice with the pomace fermentation liquor to obtain mixed liquor, and performing enzymolysis on the mixed liquor by adopting pectinase and papain to obtain an enzymatic hydrolysate; and D) separating and purifying the enzymatic hydrolysate to obtain a procyanidine extract. According to the method, pomace cell walls can be degraded to facilitate dissolution of the proanthocyanidins, so that the proanthocyanidins in the pomace are extracted to the greatest extent, the whole process conditions are mild, required equipment is simple, and large-scale and industrial production can be realized; meanwhile, waste utilization of the aronia melanocarpa pomace can be achieved through the method, development of the aronia melanocarpa deep processing industry is promoted, and the additional value of the aronia melanocarpa is increased.

Description

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Claims

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Application Information

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Owner 秦皇岛惠恩生物技术有限公司
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