Preparation method of fluorescent coding microsphere coated with metal nano shell layer

A metal nanometer, fluorescent encoding technology, applied in the field of preparation, fluorescent encoding microspheres, can solve the problems of low surface coverage, uncontrollable LSPR peaks, complex preparation process and so on
CN113337271AActive Publication Date: 2021-09-03HANGZHOU SHINEDO BIOTECH CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HANGZHOU SHINEDO BIOTECH CO LTD
Publication Date
2021-09-03

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Abstract

The invention provides a preparation method of a fluorescent coding microsphere coated with a metal nano shell layer. Different types of fluorescent materials with different contents are doped into a polymer microsphere by using a metal nano material modified with a certain ligand as a new surfactant in an emulsification process through an SPG method so as to prepare the fluorescent coding microsphere with different fluorescent coding signals inside and uniformly coated with the metal nano shell layer on the surface in one step. The prepared fluorescence coding microsphere comprises the metal nano shell layer, a polymer and a fluorescence coding material, the particle size of the fluorescence coding microsphere is 1-20 [mu]m, the coefficient of variation (CV) is less than 10%, and the fluorescence coding microsphere can be used for detecting protein / nucleic acid. The preparation method is simple in preparation process, high in surface coating rate, good in uniformity and controllable in LSPR peak, and the problems of low surface coating rate, poor uniformity, complex preparation process, uncontrollable local surface plasma resonance (LSPR) peak and the like in the current common precious metal nano shell coating method are solved.
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Description

technical field

[0001] The invention relates to the field of preparation and application of micro-nano materials, in particular to fluorescence-encoded microspheres coated with metal nano-shells, a preparation method and applications thereof. Background technique

[0002] Liquid-phase biochip technology uses encoded microspheres as the carrier of liquid-phase reactions, and according to the interaction rules of protein-protein, nucleic acid-nucleic acid, etc., uses fast and high-throughput flow cytometry as an analysis method to analyze proteins, nucleic acids, etc. Rapid and high-throughput multiplex quantitative detection has a very wide range of applications in the fields of protein expression, nucleic acid analysis, early diagnosis and prognosis of diseases. Compared with traditional detection methods, although liquid-phase chip technology has achieved higher detection sensitivity, with the rapid development of nanoscience and nanotechnology, especially in the face of th...

Claims

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