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Fungus strain for promoting germination of Liparis campylostalix seeds, germination accelerant and application

The technology of a goat's ear and an accelerator is applied in the field of a germination accelerator and a fungal strain that promotes the germination of a goat's ear, and can solve problems such as germination difficulties, seeds lacking endosperm, and no goat's ear, etc.

Active Publication Date: 2021-09-03
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Sheep's ear garlic has high medicinal value. The medicinal part is the whole plant with roots, and the market demand is large. However, at present, it is mainly obtained from wild collection, and there is no report of artificial cultivation. High requirements for conditions have caused serious damage to the wild plant resources of sheep ear garlic
[0004] Sheep ear garlic has a large amount of seeds, but due to the tiny seeds and lack of endosperm, it is very difficult to germinate under natural conditions, the germination rate is extremely low, less than 0.01%, and it needs suitable mycorrhizal fungus symbiosis to germinate, which is greatly affected by environmental factors
Therefore, it is extremely important to obtain a method for promoting the germination of Allium mutton, and there is no research about utilizing fungi to promote the germination of Allium mutton in the prior art

Method used

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  • Fungus strain for promoting germination of Liparis campylostalix seeds, germination accelerant and application
  • Fungus strain for promoting germination of Liparis campylostalix seeds, germination accelerant and application

Examples

Experimental program
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Effect test

Embodiment 1

[0030] 1. Test materials and methods

[0031] 1. Test materials

[0032] Sheep's ear garlic plant: the wild sheep's ear garlic plant was obtained from Dongling Park, Shenyang City, Liaoning Province.

[0033] Fungal strains: obtained by isolation and screening.

[0034] 2. Strain Isolation

[0035] Take the root of the sheep ear garlic plant, wash and disinfect the surface, cut the root section into small pieces of about 3mm, and place it in PDA medium for cultivation at 25°C. After one week, observe the growth of the colony around the root section, pick The marginal hyphae were transferred to a new PDA medium for separate purification and culture at a temperature of 25°C. After the 90mm petri dish was covered, the DNA was extracted to identify the fungal species.

[0036] 3. Germination rate experiment

[0037] Experimental group (28 days): do three repetitions, respectively numbered as a, b, c, select 5 seed pods respectively, soak the complete seed pods in distilled wat...

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Abstract

The invention relates to the technical field of microbe application, and particularly discloses a fungus strain for promoting germination of Liparis campylostalix seeds, the strain is No. 65, the preservation date of the strain is March 29, 2021, the preservation number of the strain is CGMCC 21937, and the strain is classified and named as Tulasnella sp. The strain 65 obtained by the invention has a promoting effect on the germination of the lysimachia christinae seeds, so that the germination rate of the Liparis campylostalix seeds reaches 85.24%.

Description

technical field [0001] The invention relates to the technical field of microbial application, in particular to a fungal strain for promoting the germination of sheep's ear garlic seeds, a germination accelerator and its application. Background technique [0002] Sheep's ear garlic (Liparis campylostalix) is the rooted whole herb of the orchid plant Liparis campylostalix, which is distributed in the northeast, northwest and Anhui, Hubei, Sichuan, Guizhou, Yunnan and other places. It has the effects of promoting blood circulation and stopping bleeding, reducing swelling and relieving pain. Commonly used for metrorrhagia, postpartum abdominal pain, excessive leucorrhea, tonsillitis, bruises, burns. [0003] Sheep's ear garlic has high medicinal value. The medicinal part is the whole plant with roots, and the market demand is large. However, at present, it is mainly collected from the wild, and there is no report of artificial cultivation. The requirement of the conditions is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01C1/06C12R1/645
CPCC12N1/14A01C1/06
Inventor 陈旭辉刘丹丁锐林浩吴海红孟围围路巽然王恺婷曲波张丽杰
Owner SHENYANG AGRI UNIV