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A pdx model cell of human skin leukemia and its application

A technology of model cells and leukemia, applied in the field of cell models, can solve the problems of few sources of specimens, small number of cells, high heterogeneity, etc., and achieve the effect of good promotion and application value

Active Publication Date: 2022-08-02
XIN HUA HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the few sources, high heterogeneity, low cell number, and low engraftment rate of cutaneous leukemia specimens, there is no stable PDX model for research.

Method used

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  • A pdx model cell of human skin leukemia and its application
  • A pdx model cell of human skin leukemia and its application
  • A pdx model cell of human skin leukemia and its application

Examples

Experimental program
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Embodiment Construction

[0025] The present invention will be further described below in conjunction with specific embodiments. The experimental methods used in the following examples are conventional methods unless otherwise specified. The materials, reagents, etc. used in the following examples can be obtained from commercial sources unless otherwise specified. It should be understood that these examples are only used to illustrate the present invention and not to limit the scope of the present invention.

[0026] 1. Sample processing of skin leukemia patients:

[0027] 1. Collect subcutaneously punctured tumor tissue samples from patients with cutaneous leukemia, wash twice with normal saline, resuspend in 0.5ml volume of normal saline, and count the cells.

[0028] 2. Add matrigel (purchased from BD, USA) in a volume ratio of 1:1, incubate on ice for 20 minutes, and inject it into mice subcutaneously, the number of inoculated cells is 1×10 7 / Only.

[0029] 2. Raising and inoculation of NSG mi...

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PUM

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Abstract

The present invention provides a PDX model cell of human skin leukemia and its application. The name of the cell is LCutisSK-PDX cell, and the deposit number is CCTCC NO: C202165. The invention establishes a skin leukemia PDX model cell which can be stably passaged, the cytogenetic change is consistent with that of the patient, and can be stably colonized in the skin. Intravenous injection or subcutaneous injection of the cells can cause skin leukemia in model mice, which is manifested as multiple skin masses, accompanied by infiltration of leukemia cells in organs such as bone marrow and spleen. Using the PDX mouse tumor tissue, in vitro compound screening can be performed to obtain sensitive compounds for skin leukemia. This cell model provides an important preclinical research model for studying the pathogenesis of human skin leukemia and screening new targets and small molecule drugs, and has a good promotion and application value.

Description

technical field [0001] The invention relates to the technical field of cell models, in particular to a PDX model cell of human skin leukemia and its application. Background technique [0002] Cutaneous leukemia refers to the infiltration of abnormally proliferated leukemia cells into the skin, resulting in the appearance of specific or non-specific skin lesions. Cutaneous leukemia can occur simultaneously with the involvement of systemic organs, including the spleen, liver, and lymph nodes, in patients with leukemia, or before or after the diagnosis of leukemia. Simple cutaneous leukemia, also known as primary cutaneous leukemia, is relatively rare and is often an early manifestation of systemic leukemia. [0003] The shape of specific skin lesions of cutaneous leukemia has various manifestations, mostly papules, nodules or masses. The color of the skin lesions can be tan, red, purple or blue; the skin lesions can be all over the body, and the lips and gums can be seen, an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/09C12Q1/02A01K67/027
CPCC12N5/0694G01N33/5011A01K67/0271C12N2503/02G01N2500/10A01K2207/12A01K2227/105A01K2267/0331
Inventor 卢莹闫金松姚志荣李忆东江月杨立雪
Owner XIN HUA HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE