Novel composition
A technology of oil composition and biomass, which is applied in the direction of drug combination, animal feed, animal feed, etc., and can solve the problems of reducing VFA energy and carbon content
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Embodiment 1
[0203] Embodiment 1: the preparation of jellyfish oil composition
[0204] M. yewus (gametophyte stage) was collected from Magnetic Island (QLD, Australia). Asparagus sp. was collected from Cloudy Bay, Bruny Island, Tasmania (43°.43'94"S; 147°.21'.52"E) from Cloudy Bay, Bruny Island, Tasmania. Fresh biomass was blotted dry and samples (6 x 30.0 g fresh weight (fw)) were collected into individual 250 mL glass bottles (Schott), each pre-filled with 100 mL of vegetable oil (Homebrand blended vegetable oil, mustard Flower Seed Oil 95%, Sunflower Oil 5%). Comparative samples (6 x 30.0 g fresh weight (fw)) were also collected into 100 mL of milliQ water instead of vegetable oil. All vials were securely capped and stored on ice for immediate transport to the laboratory for further processing.
[0205] Biomass samples (6 x 30.0 g fw) were also collected into zip lock bags for dry weight (dw) determination and bromoform content in the biomass (3 x 30.0 g fw).
[0206] In the labora...
Embodiment 2
[0219] Example 2: Shelf-Life of Asparagus Oil Compositions
[0220] Shelf life (eg, stability) of the Asparagus oil compositions of the present invention was evaluated. As a function of time, the ratio of bioactive compounds such as bromoform (BF) and dibromochloromethane (DBCM) was investigated and compared to an internal standard (IS; naphthalene). Any decrease in the ratio indicates a loss of the compound, and thus a decrease in the stability of the bioactive oil composition, resulting in a shortened shelf life.
[0221] After 10 days of extraction of Asparagus biomass into oil or water, the biomass was removed. The Asparagus oil composition (with biomass) was filtered through a 100 μm nylon mesh screen followed by centrifugation (3200 g, 15 minutes). Each replicate clear solution from each treatment (oil or water) was then split into two 30 mL glass bottles (Schott), where one of these bottles was stored at 4°C and the other was stored at 25°C (each temperature n=3 for ...
Embodiment 3
[0230] Example 3: Maximizing Biomass Extraction from Oil
[0231] Using the extraction method of Example 1 (provided the highest concentration of bioactive agent (bromomethane) detected in the oil in the shortest amount of time (e.g. homogenization and extraction into the oil)) for the extraction of The maximum amount of Asparagus biomass was quantified.
[0232] M. yewus (gametophyte stage) was collected from Magnetic Island (QLD, Australia). Fresh biomass was blotted dry and samples of the following masses were collected into separate 250 mL glass bottles pre-filled with 100 mL vegetable oil: 30.0 g, 60.0 g, 90.0 g and 120.0 g fw (n=3 for each amount ). All vials were securely capped and stored on ice for immediate transport to the laboratory for further processing and analysis. Duplicate samples (n=3) of blotted biomass (30.0 g fw) were also collected for dry weight determination as shown in the experimental design below:
[0233]
[0234] In the laboratory, biomass ...
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