S-shaped optical fiber taper immunosensor, as well as preparation method and application thereof

A technology of immunosensor and fiber optic cone, which is applied in the field of fiber optic biosensors, can solve the problems of low sensitivity and specificity of the refractive index, and achieve the effects of wide application prospects, simple preparation process and strong specificity

Active Publication Date: 2021-09-17
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the structure of the above optical fiber biosensors is relatively simple a

Method used

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  • S-shaped optical fiber taper immunosensor, as well as preparation method and application thereof
  • S-shaped optical fiber taper immunosensor, as well as preparation method and application thereof
  • S-shaped optical fiber taper immunosensor, as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A graphene oxide / gold nanoparticle functionalized S-shaped fiber optic cone immunosensor based on single-mode fiber (SMF-28e) was fabricated by combining arc discharge technology of fusion splicer and in situ self-assembly technology. The preparation process of the optical immunosensor is simple, low in cost, high in specificity and sensitivity, and has wide application prospects.

[0035] The specific preparation steps of the S-shaped fiber optic cone immunosensor are as follows:

[0036] (1), preparation of S-shaped fiber optic taper

[0037]The specific preparation steps are as follows: ① A section of 50 cm long single-mode optical fiber is cut, and its parameters are as follows: the core diameter is 9 μm, the cladding diameter is 125 μm, and the coating layer diameter is 250 μm. Then use optical fiber pliers to peel off 3 cm of the coating layer in the middle part, and wipe clean the optical fiber with the stripped coating layer along the same direction of the fibe...

Embodiment 2

[0044] Example 2: Carcinoembryonic Antigen Detection

[0045] Step (1) → (3) is the same as embodiment 1

[0046] Step (4), one end of the S-shaped fiber cone structure is connected to a broadband light source (Danish NKTPhotonics company, SuperkCompact), and the other end is connected to a spectrum analyzer (Japan Yokogawa company, AQ6370D), and the resolution of the spectrum analyzer is set to 0.02nm, The wavelength scanning range is 1000nm-1700nm. Among them, the broadband light source outputs supercontinuum light, and the spectrum analyzer monitors the transmission spectrum during antigen detection in real time. The concentrations of carcinoembryonic antigen were set to 0nM, 0.2nM, 0.4nM, 0.6nM, 0.8nM, 1.0nM, 1.2nM and 1.4nM, respectively, and the concentration was detected from low to high during detection.

[0047] Figure 5 It is a schematic diagram of S-shaped fiber optic cone immunosensor detection.

[0048] Figure 6 is the transmission spectrum of the prepared ...

Embodiment 3

[0049] Embodiment 3: Specificity detection and repeatability detection

[0050] First, the specific detection of the S-shaped fiber optic cone immunosensor was verified, and the specific experimental steps were as follows:

[0051] Step (1)→(3) is the same as embodiment 2.

[0052] Step (4), one end of the S-shaped fiber cone structure is connected to a broadband light source (Denmark NKTPhotonics company, SuperkCompact), and the other end is connected to a spectrum analyzer (Japan Yokogawa company, AQ6370D), and the resolution of the spectrum analyzer is set to 0.02nm, The wavelength scanning range is 1000nm-1700nm. Among them, the broadband light source outputs supercontinuum light, and the spectrum analyzer monitors the transmission spectrum during antigen detection in real time. The analyte to be detected was sequentially replaced with phosphate buffered saline, bovine serum albumin, and sheep serum albumin to observe the spectral changes to verify that the spectral shif...

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Abstract

The invention discloses an S-shaped optical fiber taper immunosensor as well as a preparation method and application thereof, and belongs to the field of optical fiber biosensors. Graphene oxide and gold nanoparticles are self-assembled to the surface of an S-shaped optical fiber taper to serve as a composite biological sensitive film; the graphene oxide is fully connected with the surface of the optical fiber, so that a biosensing supporting surface is increased, and subsequent biochemical molecular connection can be facilitated; the gold nanoparticles are combined with the graphene oxide and can also be firmly combined with a nano antibody, the effects of biological connection and signal amplification are achieved; and the two materials are combined to prepare the composite sensitive film, and the composite sensitive film is connected with the nano antibody with small volume, high affinity and strong specificity, so that specific recognition and combination of a carcino-embryonic antigen is realized,so that the effects of improving the specificity, stability and sensitivity of antigen detection are achieved. The method for preparing the optical immunosensor is simple in preparation process, low in cost and short in period; the sensitivity, specificity and stability of the immunosensor are improved, the response of theimmunosensor is fast, and label-free online detection can be achieved.

Description

technical field [0001] The invention belongs to the field of optical fiber biosensors, and in particular relates to an S-shaped optical fiber cone immune sensor, a preparation method and an application thereof. Background technique [0002] Tumor marker detection is an indispensable operation in the diagnosis of malignant tumors, which can improve the accuracy of tumor diagnosis. As one of the common tumor markers, carcinoembryonic antigen is overexpressed in many human cancers, and has very important clinical value in the differential diagnosis of malignant tumors, efficacy evaluation and disease monitoring. Optical fiber biosensing technology is a multidisciplinary optical detection technology obtained by combining optical fiber sensing technology with biological specific recognition technology, which can be used in the field of biochemical molecular detection. As a commonly used optical fiber structure, sensing devices based on the principle of optical fiber intermode in...

Claims

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Application Information

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IPC IPC(8): G01N33/543
CPCG01N33/54346
Inventor 于永森郭万美国旗王博金光勇
Owner JILIN UNIV
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