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Kinase activity regulation mechanism of G protein coupled receptor kinase 6

A technology of coupled receptor and kinase activity, applied in the field of G protein coupled receptor kinase 6 kinase activity regulation, to achieve the effect of improving kinase activity, increasing phosphorylation and treating Parkinson's disease

Inactive Publication Date: 2021-09-21
深圳小海豚医药科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the molecular mechanism of how NO regulates GRK4 subfamily signaling remains incompletely understood

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  • Kinase activity regulation mechanism of G protein coupled receptor kinase 6
  • Kinase activity regulation mechanism of G protein coupled receptor kinase 6

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Embodiment Construction

[0017] In order to enable those skilled in the art to better understand the present invention, the technical solution of the present invention will be further described through the following examples.

[0018] The GRK6A gene was cloned from the gene bank, and GRK6 mutants were generated by site-directed mutagenesis using the fusion PCR method;

[0019] The primer sequences for cloning and generating GRK6 gene mutations are as follows:

[0020] GRK6-EcoR I F:CCggAATTCATggAgCTCgAgAACATCg

[0021] GRK6-Sal I R:TgCgCAgCTgCTACCGCCAACTGCTGGTGG

[0022] GRK6-C201A F:GCAAAGGTGGCTTTGGGGAGGTGGCCGCCTGCCAGGTGCGGGCCACAG

[0023] GRK6-C201A R:CTGTGGCCCGCACCTGGCAGGCGGCCACCTCCCCCAAAGCCACCTTTGC

[0024] GRK6-C214A F:CCACAggTAAgATgTATgCCgCCAAgAAgCTAgAgAAAAgCg

[0025] GRK6-C214A R:CgCTTTTCTCTAgCTTCTTggCggCATACATCTTACCTgTgg

[0026] GRK6-C260A-R:CTATGAGACCAAGGACGCGCTGGCCCTGGTGCTGACACTGATGAACG

[0027] GRK6-C260A-R:CGTTCATCAGTGTCAGCACCAGGGCCAGCGCGTCCTTGGTCTCATAG

[0028] GRk6-C297A-F:GTCTT...

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Abstract

The invention belongs to the technical field of prevention and treatment of Parkinson's disease, and particularly relates to a kinase activity regulation method of G protein coupled receptor kinase 6. The invention discloses a kinase activity regulation mechanism of G protein-coupled receptor kinase 6, the G protein-coupled receptor kinase 6 (GRK6) gene is cloned from a gene bank, and the cloned GRK6 is converted into a GRK6 mutant through site-specific mutagenesis by using a fusion PCR method; wherein the activity regulation and control site of the GRK6 is a Cys474 site; a primer sequence for cloning GRK6 gene mutation is GRk6-C474A-F: GCCTGACCCCCAGGCCATTTACGCCAAGGATGTTCTGGACATTGAACAGTTCTC, and a primer sequence for generating the GRK6 mutant is GRk6-C474A-R:GAGAACTGTTCAATGTCCAGAACATCCTTGGCGTAAATGGCCTGGGGGTCAGGC.

Description

technical field [0001] The invention belongs to the technical field of prevention and treatment of Parkinson's disease, and in particular relates to a method for regulating the kinase activity of G protein-coupled receptor kinase 6. Background technique [0002] Parkinson's disease (PD), or simply Parkinson's, is a long-term degenerative disease of the central nervous system primarily affecting the motor system. Symptoms usually come on slowly, and nonmotor symptoms become more prevalent as the disease progresses. The most obvious early symptoms are tremors, stiffness, slowness of movement and difficulty walking. Parkinson's disease dementia becomes common in the advanced stages of the disease. People with Parkinson's may also have problems with sleep and sensory systems. The motor symptoms that result from the disease result in the death of cells in the substantia nigra, an area of ​​the midbrain, that lead to a decrease in dopamine. The cause of this cell death is uncl...

Claims

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Application Information

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IPC IPC(8): C12N15/54C12N15/10A61P25/16
CPCC12N9/12C12N15/1031A61P25/16C12Y207/11016C12Q2531/113
Inventor 余培春余培清
Owner 深圳小海豚医药科技有限公司
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