Special culture medium for hirsutella sinensis and preparation method thereof

A Chinese Trichospora and culture medium technology, applied in the biological field, can solve the problems of complex physiological characteristics of Cordyceps sinensis, inability to grow in medium, complex physiological characteristics of Trichosporium, and achieve a short lag period, improved efficiency, and rapid growth. Effect

Inactive Publication Date: 2021-09-24
GANSU AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Realizing the artificial cultivation of Cordyceps sinensis has always been the common dream of researchers of Cordyceps sinensis, and many researchers have made unremitting efforts for this. However, this dream has not been realized until now. The reason is that the physiological characteristics of Cordyceps sinensis itself complexity of
Existing research data show that Hirsutella sinensis is an anamorph of...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The special culture medium for Mortierella chinensis provided in this example takes 1000mL as a unit, and the raw material components are mainly 400mL of beef juice, 200mL of fresh milk, 0.5 grams of compound vitamin B, 1 gram of dipotassium hydrogen phosphate, and 0.5 grams of sodium chloride. gram, biotin 0.5g, hydrolyzed milk protein 5g, nucleic acid 0.5g, yeast powder 1g, agar 16g, water 400mL.

[0026] The preparation method of the Chinese Mortierella special culture medium is as follows:

[0027] ① Prepare the equipment needed for the experiment;

[0028] ②. Accurately measure or weigh 400mL of beef juice, 200mL of fresh milk, 1g of dipotassium hydrogen phosphate, 0.5g of sodium chloride, 5g of hydrolyzed milk protein, 0.5g of nucleic acid, and 1g of yeast powder in a 1000mL beaker;

[0029] ③. Add 300mL water to the above-mentioned beaker, heat the beaker in an electric heating mantle, and stir continuously with a glass rod to fully dissolve the drug. After boil...

Embodiment 2

[0040] The special culture medium for Mortierella chinensis provided in this example takes 1000mL as a unit, and the raw material components are mainly 400mL of beef juice, 200mL of fresh milk, 0.5 grams of compound vitamin B, 1 gram of dipotassium hydrogen phosphate, and 0.5 grams of sodium chloride. gram, biotin 0.5g, hydrolyzed milk protein 5g, nucleic acid 0.5g, yeast powder 1g, agar 16g, water 400mL.

[0041] The preparation method of the Chinese Mortierella special culture medium is as follows:

[0042] ① Prepare the equipment needed for the experiment;

[0043]②. Accurately measure or weigh 400mL of beef juice, 200mL of fresh milk, 1g of dipotassium hydrogen phosphate, 0.5g of sodium chloride, 5g of hydrolyzed milk protein, 0.5g of nucleic acid, and 1g of yeast powder in a 1000mL beaker;

[0044] ③. Add about 300mL of water to the above beaker, heat the beaker in an electric heating mantle, and stir continuously with a glass rod to fully dissolve the drug. After boilin...

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Abstract

The invention discloses a special culture medium for hirsutella sinensis and a preparation method thereof, and belongs to the field of biotechnology. 1000 mL of the culture medium is mainly prepared from 400 mL of beef juice, 200 mL of fresh milk, 0.5 g of compound vitamin B, 1 g of dipotassium phosphate, 0.5 g of sodium chloride, 0.5 g of biotin, 5 g of lactoalbumin hydrolysate, 0.5 g of nucleic acid, 1 g of yeast powder, 16 g of agar and 400 mL of water. The culture medium disclosed by the invention has the advantages of short delay phase of the inoculated hirsutella sinensis, rapid growth, robust hypha, stable hypha character, stable yield and the like, and is a very mature and stable culture medium formula in the research of the hirsutella sinensis.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a special medium for Mortierella chinensis and a preparation method thereof. Background technique [0002] Cordyceps sinensis, also known as Cordyceps sinensis, is one of the precious and nourishing Chinese medicinal materials. It is also known as the "three treasures" of Chinese traditional medicine together with velvet antler and ginseng. It is a complex of insects and bacteria formed after Cordyceps sinensis infects the larvae of bat moths. Cordyceps sinensis in the world is only distributed in alpine meadows at an altitude of 2800-5000m in four countries around the Qinghai-Tibet Plateau, including China, Nepal, Bhutan and India. However, the distribution of Cordyceps sinensis mentioned in some domestic articles is not correct in Shanxi, Henan, Taiwan and other countries or regions such as the United States and Japan, because other types of Cordyceps are mistaken for ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 赵树栋李建宏
Owner GANSU AGRI UNIV
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