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Reticulocyte detection dye, detection reagent, preparation method of detection reagent, detection method of sample analyzer and sample analyzer

A technology of reticulocytes and detection reagents, which is applied in the field of blood cell analysis and can solve problems such as the inability to meet the requirements of automated testing

Active Publication Date: 2021-11-30
SHENZHEN DYMIND BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, it cannot meet the requirements of automated testing

Method used

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  • Reticulocyte detection dye, detection reagent, preparation method of detection reagent, detection method of sample analyzer and sample analyzer
  • Reticulocyte detection dye, detection reagent, preparation method of detection reagent, detection method of sample analyzer and sample analyzer

Examples

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preparation example Construction

[0052] The present invention also provides a method for preparing a reticulocyte detection reagent, the method comprising: after configuring the above-mentioned detection dye and the diluent in a certain proportion to a preset volume, stirring evenly at room temperature, using 0.22 um filter membrane for filtration, and the filtrate after filtration is tested for the sample.

[0053] The present invention also provides a detection method for a sample analyzer. The method includes the following steps: using the reticulocyte detection reagent as described above, treating the sample with the detection reagent, and the detection reagent transforms the reticulocyte in the sample into a spherical shape. to enable the nucleic acid of the reticulocytes to specifically combine with the fluorescent dye, thereby realizing the counting of the reticulocytes.

[0054] The present invention also provides a sample analyzer adopting the detection method of the sample analyzer, the sample analy...

Embodiment example 1

[0058] Reagent name concentration Phosphate buffer pair 3.6g / L sodium hydroxide 2.1 g / L sodium sulfate 0.8g / L Decyl sultaine 1.1 g / L Octyltrimethylammonium Chloride 1.6g / L Isothiazolinone 0.04g / L pure water Dilute to 1000mL

[0059] Reagent name concentration Dye 1 0.0035 g Isopropanol 3.0 g Ethylene glycol Quantitative to 100g

[0060] Prepare the diluent and detection dye as shown in the above table and dilute to the specified amount, stir evenly at room temperature, and filter with a 0.22um filter membrane. Use the Sysmex XN1000 instrument to compare the supporting reagents with the self-made reagent models. See Table 1 for details of the results.

[0061] Table 1:

[0062] RET% Invention RET%XN-1000 Absolute deviation (no more than ±0.3) LFR Invention LFR XN-1000 Relative deviation (no more than ±30%) MFR Invention MFR XN-1000 Relative deviation (no...

Embodiment example 2

[0065] Reagent name concentration Phosphate buffer pair 3.6g / L sodium hydroxide 2.1 g / L sodium sulfate 0.8g / L Decyl sultaine 1.1 g / L Octyltrimethylammonium Chloride 1.6g / L Isothiazolinone 0.04g / L pure water Dilute to 1000mL

[0066] Reagent name concentration Dye 2 0.003 g Isopropanol 3.0 g Ethylene glycol Quantitative to 100g

[0067] Prepare the diluent and detection dye as shown in the above table and dilute to the specified amount, stir evenly at room temperature, and filter with a 0.22um filter membrane. Use the Sysmex XN1000 instrument to compare the supporting reagents with the self-made reagent models. See Table 2 for details of the results.

[0068] Table 2:

[0069] RET% Invention RET%XN-1000 Absolute deviation (no more than ±0.3) LFR Invention LFR XN-1000 Relative deviation (no more than ±30%) MFR Invention MFR XN-1000 Relative deviation (no ...

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Abstract

The invention provides a reticulocyte detection dye, a detection reagent, a preparation method of a detection reagent, a sample analyzer detection method, and a sample analyzer. The provided nucleic acid dye can be used in a blood analyzer to meet the requirements for accurate and rapid counting of reticulocytes. testing requirements. The reticulocyte count is treated with reagents, adding specific reticulocyte nucleic acid dyes and reticulocyte cell membrane processing reagents, and using nucleic acid fluorescent staining and semiconductor laser flow cytometry to accurately count reticulocytes in a single channel, the RET channel. red blood cells. That is, the diluent is used to treat the shape of reticulocytes, and at the same time to spheroidize them, change the shape of various types of cell membranes, so as to facilitate the specific combination of nucleic acid of reticulocytes and dyes, so as to realize the counting of reticulocytes.

Description

technical field [0001] The invention relates to the technical field of blood cell analysis, in particular to a reticulocyte detection dye, a detection reagent, a preparation method of the detection reagent, a sample analyzer detection method and a sample analyzer reagent. Background technique [0002] Reticulocyte count (especially the absolute value of reticulocyte) is an important index reflecting the hematopoietic function of bone marrow. The current detection methods mainly include manual method and flow cytometry. [0003] Manual method: Use brilliant tar blue and other vital staining methods to display the reticular structure in red blood cells, and count the number of reticulocytes in 1000 red blood cells under a microscope, expressed in percentage or fraction. Although the amount of blood taken by this method is small, the water in the mixed blood is easy to evaporate during staining, resulting in a short staining time, low results and more affected by subjective fa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N15/14G01N1/30G01N1/38C09B23/06C09B23/10
CPCG01N15/1434G01N1/30G01N1/38C09B23/06C09B23/105
Inventor 周贤波吕碧玉郑剑通吴忠芬
Owner SHENZHEN DYMIND BIOTECH
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