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Auxiliary device for cryopreservation

A cryopreservation and operation technology, applied in the preservation of human or animal bodies, animal husbandry, medical science, etc., can solve the problems of high difficulty, complicated process, difficult to confirm and place in containers for cryopreservation, and achieve rapid impregnation Effect

Inactive Publication Date: 2021-09-28
MITSUBISHI PAPER MILLS LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is difficult to attach embryos or eggs to the inner surface of cryopreservation containers such as cryopreservation straws, cryovials, or cryovials, and it is difficult to confirm that embryos or eggs are reliably placed in cryopreservation containers.
In addition, a straw sealer is required in the freezing process, and a straw cutter is also required in the thawing process, which requires special equipment and becomes a complicated process.

Method used

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  • Auxiliary device for cryopreservation
  • Auxiliary device for cryopreservation
  • Auxiliary device for cryopreservation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0140] Using polycarbonate resin with a total light transmittance of 85%, it is produced by cutting figure 1 The shown cryopreservation operation auxiliary jig 16 has a cover member holding portion 2, a main body member holding portion 3, and an observation portion 4 having an opening structure. Regarding the size of the base material 1 [vertical x horizontal x height (thickness)], it is 23 mm x 80 mm x 3mm, and the right side (the range where the main body member holding portion 3 is formed) is 23mm×90mm×4mm. The width×length×depth of the cover member holding portion 2 is 3.2 mm×35 mm×1 mm. The width×length×depth of the main body member holding portion 3 is 3.2 mm×90 mm×2 mm. The width×length of the observation portion 4 having the opening structure is 5 mm×40 mm. In addition, the height (thickness) of the bottom surface of the cover member holding part 2 and the bottom surface of the main body member holding part 3 from the bottom surface of the base material 1 was 2 mm. ...

Embodiment 2

[0142] Make the overall height of the base material 1 uniform, as a whole, set the vertical x horizontal x height to 23 mm x 170 mm x 3 mm, and change the depth of the groove part of the main body member holding part 3 to 1 mm. In the same manner as in Example 1, the cryopreservation aid jig of Example 2 was produced. The height (thickness) of the bottom surface of the lid member holding portion 2 and the bottom surface of the main body member holding portion 3 from the bottom surface of the base material 1 is 2 mm.

Embodiment 3

[0144] Manufactured by cutting using polycarbonate resin Figure 8 The shown cryopreservation operation auxiliary jig has a cover member holding portion 2, a main body member holding portion 3, and an observation portion 4 with an opening structure. The size of the substrate 1 [vertical x horizontal x height (thickness)] is 23 mm x 80 mm from the left end to 80 mm in the right direction (horizontal direction) (the range where the cover member holding part 2 and the observation part 4 with the opening structure are formed) ×3mm, and the right side (the range where the main body member holding portion 3 is formed) is 23mm×90mm×4mm. The width * length * depth of the groove part that cover member holding part 2 has is 3.2mm * 35mm * 1mm, the width * length * depth of the groove part that main body member holding part 3 has is 3.2mm * length * 2mm, has opening The width×length of the opening of the observation unit 4 of the structure is 2.8 mm×40 mm. The observation portion 4 hav...

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PUM

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Abstract

The present invention relates to an auxiliary device for cryopreservation that is capable of stably holding a device for cryopreservation, facilitates the coverage of a cell or tissue deposited on a strip with a cap without allowing the cap to come into contact with the cell or tissue, and enables quick immersion of the device for cryopreservation in liquid nitrogen. The auxiliary device for cryopreservation, includes a substrate; a cap holder; an observation section having an aperture structure; and a device body holder, wherein the cap holder, the observation section having the aperture structure, and the device body holder are arranged in the substrate in a substantially straight line.

Description

technical field [0001] The present invention relates to a cryopreservation auxiliary jig suitable for use in the freezing operation of cells or tissues. Background technique [0002] Excellent preservation techniques for cells or tissues are required in various industrial fields. For example, in the bovine embryo transfer technique, the embryos are cryopreserved, and the embryos are thawed in accordance with the estrous cycle of the embryo recipient bovine for transplantation. In addition, in the treatment of human infertility, eggs or ovaries are collected from the mother's body, frozen and stored in advance to suit the timing for transplantation, and thawed for use at the time of transplantation. [0003] Usually, cells or tissues collected from a living body will gradually lose activity or undergo changes in shape and quality even in a culture medium, so it is not preferable to culture cells or tissues outside a living body for a long time. Therefore, techniques for lon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0268A01N1/0242A61D19/02
Inventor 松泽笃史
Owner MITSUBISHI PAPER MILLS LTD