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Construction method of SARS-CoV-2 gastrointestinal inflammation animal model

A technology of animal models and construction methods, applied in medical science, veterinary instruments, etc., can solve the problems of lack of animal models and unclear system characteristic mechanism

Inactive Publication Date: 2021-09-28
THE FIFTH AFFILIATED HOSPITAL SUN YAT SEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the systemic characterization of susceptible sites in the gastrointestinal tract of COVID-19 patients and the mechanisms underlying the development of gastrointestinal symptoms remain unclear, partly due to the lack of animal models specifically designed for SARS-CoV-2 gastrointestinal infection

Method used

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  • Construction method of SARS-CoV-2 gastrointestinal inflammation animal model
  • Construction method of SARS-CoV-2 gastrointestinal inflammation animal model
  • Construction method of SARS-CoV-2 gastrointestinal inflammation animal model

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Experimental program
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Embodiment 1

[0043] The construction of embodiment 1 animal model

[0044] Include the following steps:

[0045] (1) C57BL / 6J mice at 8 to 9 weeks were randomly divided into two groups (experimental group and control group);

[0046] (2) All mice were fasted for 24 hours;

[0047] (3) After fasting for 24 hours, each mouse was anesthetized with isoflurane;

[0048] (4) After 5-10 minutes of anesthesia, inject 500 μL-1000 μL of 1% acetic acid from the anus, and inject an equal volume of PBS into the control group;

[0049] (5) Stand the mouse upside down for 1 min, then lavage with 500-1000 μL of PBS twice, in order to wash off the injected acetic acid;

[0050] (6) mice are put back into cages, supplemented with food;

[0051] (7) After 16 hours, intraperitoneally inject S protein (SARS-CoV-2 Spike Protein) 5 μg / monkey (or 5.5nmol / kg), and the control group is injected with isotype control Mouse IgG1-Fc Protein;

[0052] (8) After 6 hours, the mice were killed, and the duodenum, colon...

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Abstract

The invention belongs to the technical field of animal model construction, and discloses a construction method of an animal model of gastrointestinal inflammation caused by SARS-CoV-2, which comprises the following steps that: before virus infection is simulated, acid enema treatment on gastrointestinal tract epithelium is performed at first; added acetic acid can cause slight injury; after the acid enema treatment is performed, the SARS-CoV-2Spike Protein is injected into the intraperitoneal cavity, so that the reappearance of the viral gastrointestinal symptoms of a mouse can be simulated; the animal model obtained by the method is consistent with clinical symptoms of a patient related to COVID-19 reported in the prior art, so that the animal model constructed by the method disclosed by the invention can copy gastrointestinal manifestations of the clinical COVID-19 patient.

Description

technical field [0001] The present invention relates to the technical field of animal model construction, and more specifically, to a method for constructing an animal model of SARS-CoV-2 gastrointestinal inflammation. Background technique [0002] COVID-19, the severe acute respiratory disease caused by SARS-CoV-2, has caused a worldwide pandemic since its outbreak. Typical respiratory symptoms have been identified as a common feature of SARS-CoV-2 infection, and the airway was identified as a target organ. However, since our research group provided the first evidence of gastrointestinal tract, and with the in-depth study of SARS-CoV-2 infection, the clinical reports of cases with gastrointestinal symptoms, which usually manifest as diarrhea, anorexia, nausea and vomiting. In addition, it is unexplained that some patients infected with SARS-CoV-2 showed only digestive system symptoms at the time of onset or even during the course of the disease, indicating the high suscep...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D7/00
CPCA61D7/00
Inventor 何欢欢曾发敏何建忠李颖雯邓昭华
Owner THE FIFTH AFFILIATED HOSPITAL SUN YAT SEN UNIV