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Method for simultaneously extracting multiple active substances in porphyridium

A technology of Porphyra algae and extract, applied in the field of bioengineering, can solve problems such as not mentioned, and achieve the effects of high antioxidant activity, high extraction efficiency and simple operation

Pending Publication Date: 2021-10-08
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The patent does not mention the subsequent utilization of algae residue after extraction of phycoerythrin

Method used

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  • Method for simultaneously extracting multiple active substances in porphyridium
  • Method for simultaneously extracting multiple active substances in porphyridium
  • Method for simultaneously extracting multiple active substances in porphyridium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 extracts phycoerythrin, polyunsaturated fatty acid and intracellular polysaccharide from Porphyridium algae powder (1.000g)

[0031] Extraction process (such as figure 1 (shown): at 4°C, weigh 1.000g of dried Porphyridium algae powder (initial algae powder), add 50mL of 4°C deionized water, stir for 6 hours, centrifuge at 6000 rpm for 5 minutes, and separate the phycoerythrin extract ( cold water extract) and algae residue I. Add 50mL of 95% ethanol aqueous solution by volume fraction to algae residue I, stir for 6 hours, and separate the polyunsaturated fatty acid extract (ethanol extract) and algae residue II by filtering through a membrane with a pore size of 1 micron, and add 50mL of 80°C water to algae residue II again. Deionized water, at 80°C, stir for 2 hours, centrifuge at 6000 rpm for 5 minutes, centrifuge to separate intracellular polysaccharide extract (hot water extract) and algae dregs III, and dry algal dregs III at 80°C .

[0032]Yield an...

Embodiment 2

[0037] Example 2 Phycoerythrin, polyunsaturated fatty acids and intracellular polysaccharides are extracted from Porphyridium algae powder (10.00g)

[0038] Extraction process (such as figure 1 As shown): under the condition of 4°C, weigh 10.00g of dried Porphyridium algae powder (initial algae powder), add 500mL of 4°C deionized water, stir for 6 hours, centrifuge at 6000 rpm for 5 minutes, and separate the phycoerythrin extract ( cold water extract) and algae residue I. Add 500mL of 95% ethanol aqueous solution to the algae residue I, stir for 6 hours, and separate the polyunsaturated fatty acid extract (ethanol extract) and algae residue II by filtering through a membrane with a pore size of 1 micron, and add 500mL of 80°C Deionized water, at 80°C, stir for 2 hours, centrifuge at 6000 rpm for 5 minutes, centrifuge to separate intracellular polysaccharide extract (hot water extract) and algae dregs III, and dry algal dregs III at 80°C .

[0039] Yield analysis: After extr...

Embodiment 3

[0044] Example 3 Phycoerythrin, polyunsaturated fatty acids and intracellular polysaccharides are extracted from Porphyridum algae wet algae mud (100.0g)

[0045] Extraction process (such as figure 1 Shown): At 4°C, weigh 100.0g of Porphyridum wet algae mud (initial algae mud, water content 90.1%), add 400mL of 4°C deionized water, stir for 6 hours, centrifuge at 6000 rpm for 5 minutes, separate Phycoerythrin extract (cold water extract) and algae residue I. Add 400mL of 95% ethanol aqueous solution to the algae residue I, stir for 6 hours, and separate the polyunsaturated fatty acid extract (ethanol extract) and algae residue II by filtering through a membrane with a pore size of 1 micron, and add 400mL of 80°C Deionized water, at 80°C, stir for 2 hours, centrifuge at 6000 rpm for 5 minutes, centrifuge to separate intracellular polysaccharide extract (hot water extract) and algae dregs III, and dry algal dregs III at 80°C .

[0046] Yield analysis: After extraction in cold...

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Abstract

The invention discloses a method for simultaneously extracting multiple active substances in porphyridium, and belongs to the field of bioengineering. The method comprises the following steps: firstly, leaching porphyridium with cold water, separating a cold water leaching solution and porphyridium residue I, leaching the porphyridium residue I with ethanol or an ethanol aqueous solution, separating an ethanol or ethanol aqueous solution leaching solution and porphyridium residue II, leaching the porphyridium residue II with hot water, and separating a hot water leaching solution and porphyridium residue III, wherein the cold water leaching solution is a phycoerythrin extract product, the ethanol or ethanol aqueous solution leaching solution is a polyunsaturated fatty acid extract, and the hot water leaching solution is an intracellular polysaccharide extract. The extraction method is high in extraction efficiency, safe, simple to operate, non-toxic and beneficial to large-scale popularization and subsequent product development.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a method for extracting active substances in Porphyridium coccus and its application. Background technique [0002] Porphyridium purpureum is a single-celled micro-red algae that widely exists in natural environments such as oceans, freshwater lakes, and moist soil surfaces. Porphyridium can accumulate active substances such as phycoerythrin, polyunsaturated fatty acids and intracellular polysaccharides, and has potential development and utilization potential. At present, there have been a lot of reports on the extraction technology of a certain active substance in P. Unsaturated fatty acids and active polysaccharides, how to extract polyunsaturated fatty acids and intracellular polysaccharides from algae residue without destroying the structure and activity of active substances, have not been reported. [0003] The most special and most valuable substance in Porphylococcus cells ...

Claims

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Application Information

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IPC IPC(8): B01D11/02C11B1/04C11B3/00C11B1/10C07K14/405C07K1/36C07K1/34C11C1/00C08B37/00
CPCB01D11/0288B01D11/0284C11B1/04C11B3/00C11B1/10C07K14/405C11C1/002C08B37/00
Inventor 李涛向文洲吴华莲吴后波吴嘉仪
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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