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Enzyme conjugate of anti-triiodothyronine monoclonal antibody, total triiodothyronine quantitative detection kit and use method of total triiodothyronine quantitative detection kit

A technology of triiodothyroid and enzyme conjugates, which is applied in the field of immunological detection and can solve problems such as cumbersome operation and interference

Pending Publication Date: 2021-10-12
郑州达诺生物技术有限公司
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Problems solved by technology

Although this method can avoid the formation of a new combination with the triiodothyronine (T3) antigen in the matrix fluid after the binding protein in the sample dissociates, thereby causing the consequences of interfering with the competitive reaction, the physical and chemical properties of the dissociation agent have a negative effect on the enzyme. There is a certain degradation effect, so the product form of enzyme diluent and enzyme concentrate can only be used all the time, and the enzyme conjugate (present in the enzyme concentrate) and the dissociating agent (present in the enzyme diluent) are different. In the reagent components, resulting in cumbersome operations, such as preparing the enzyme conjugate working solution before use or adding samples in two steps

Method used

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preparation example Construction

[0043] The method for preparing a coated plate coated with triiodothyronine comprises the following steps: (1) adding T3-BSA to tris-HCl buffer solution to prepare a coating solution, and fully mixing;

[0044] (2) Add the prepared coating solution into a blank microtiter plate and place it at 2-8°C for 16-24hr;

[0045] (3) Shake off the coating solution in the microplate, and wash the plate at least twice with washing solution after there is no liquid on the surface of the microplate;

[0046] (4) Seal within 2 minutes after washing the plate, then add blocking solution to each well, and then place it at 2-8°C for 16-24 hours;

[0047] (5) Shake off the blocking solution of the microplate, and then place the microplate in a drying device to dry (humidity ≤ 30%) for 4-8 hours.

[0048] The preparation method of the triiodothyronine calibrator comprises the following steps: (1) using a pH of 6.8-7.5 HEPES buffer, BSA, nystatin, and Proclin300 to prepare a calibrator dilution;...

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Abstract

The invention provides an enzyme conjugate of an anti-triiodothyronine monoclonal antibody, a total triiodothyronine quantitative detection kit and a use method of the total triiodothyronine quantitative detection kit. According to the enzyme conjugate of the anti-triiodothyronine monoclonal antibody, the total triiodothyronine quantitative detection kit and the use method of the total triiodothyronine quantitative detection kit, a enzyme conjugate diluent can enhance the stability of the enzyme conjugate, meanwhile, the destructive effect of a dissociation agent on the enzyme conjugate is inhibited, the two reagents can coexist in a same reagent component, total triiodothyronine dissociation can be met at the same time in total triiodothyronine detection, interference of new and old samples and HAMA interference are reduced, therefore, the total triiodothyronine detection kit can be operated more quickly and conveniently, and more accurate detection results can be obtained.

Description

technical field [0001] The invention relates to the technical field of immunological detection, in particular to an enzyme conjugate of anti-triiodothyronine monoclonal antibody, a total triiodothyronine quantitative detection kit and a use method thereof. Background technique [0002] The molecular weight of triiodothyronine (T3) is 651, about 25% is directly produced by the thyroid gland, and 75% is formed by thyroxine (T4) in peripheral tissues through the action of 5'-deiodinase, which is currently known biological activity The strongest thyroid hormone, its biological activity is 3 to 5 times that of thyroxine. Therefore, the detection of triiodothyronine can effectively evaluate the state of thyroid function. [0003] The existing detection methods for total triiodothyronine (TT3) include liquid chromatography-tandem mass spectrometry and immunological methods. Among them, the immunological method is the mainstream method in clinical application because of its fast d...

Claims

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Application Information

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IPC IPC(8): C07K16/26G01N33/78G01N33/543G01N33/535
CPCC07K16/26G01N33/78G01N33/54306G01N33/535
Inventor 王玲玲普鹏
Owner 郑州达诺生物技术有限公司
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