Enzyme conjugate of anti-triiodothyronine monoclonal antibody, total triiodothyronine quantitative detection kit and use method of total triiodothyronine quantitative detection kit
A technology of triiodothyroid and enzyme conjugates, which is applied in the field of immunological detection and can solve problems such as cumbersome operation and interference
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[0043] The method for preparing a coated plate coated with triiodothyronine comprises the following steps: (1) adding T3-BSA to tris-HCl buffer solution to prepare a coating solution, and fully mixing;
[0044] (2) Add the prepared coating solution into a blank microtiter plate and place it at 2-8°C for 16-24hr;
[0045] (3) Shake off the coating solution in the microplate, and wash the plate at least twice with washing solution after there is no liquid on the surface of the microplate;
[0046] (4) Seal within 2 minutes after washing the plate, then add blocking solution to each well, and then place it at 2-8°C for 16-24 hours;
[0047] (5) Shake off the blocking solution of the microplate, and then place the microplate in a drying device to dry (humidity ≤ 30%) for 4-8 hours.
[0048] The preparation method of the triiodothyronine calibrator comprises the following steps: (1) using a pH of 6.8-7.5 HEPES buffer, BSA, nystatin, and Proclin300 to prepare a calibrator dilution;...
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