Molecular marker for diagnosing working of nervonic acid supplemented for ischemic hypoxic encephalopathy in plateau environment and application of molecular marker

A molecular marker, high-altitude environment technology, applied in the field of molecular markers of ischemic hypoxic encephalopathy supplemented with nervonic acid, can solve problems such as unclear

Active Publication Date: 2021-10-12
BAO FENG BIOTECH (BEIJING) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After HIE patients in the plateau environment take Yuanbaofeng nervonic acid oil, it is not clear what is the onset marker of nervonic acid, a unique component contained in it. So far, there is no clear research to prove it, and even few have research

Method used

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  • Molecular marker for diagnosing working of nervonic acid supplemented for ischemic hypoxic encephalopathy in plateau environment and application of molecular marker
  • Molecular marker for diagnosing working of nervonic acid supplemented for ischemic hypoxic encephalopathy in plateau environment and application of molecular marker
  • Molecular marker for diagnosing working of nervonic acid supplemented for ischemic hypoxic encephalopathy in plateau environment and application of molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1. 1. Ischemic-hypoxic encephalopathy model in plateau environment

[0035] Newborn 7-day-old SD rats were purchased from Liaoning Changsheng Biotechnology Co., Ltd. (license number: SCXK Liaoning 2020-0002). All mice were housed in a temperature-controlled room (22-26) with a 12-h light and dark cycle and provided free food and water throughout the study period. All male and female rats 7 days after birth were anesthetized by inhalation of ether. An incision is made in the midline of the neck. The left common carotid artery was exposed, the nerve and vein were separated, ligated with 6-0 surgical silk, and the artery between the ligation was cut off. The wound is then closed with surgical sutures and the animal is allowed to recover. The time of each operation was controlled within 5 minutes, and the temperature blanket was used to maintain the body temperature of the animals at 37°C during the operation. After recovering with the mother in the cage for 2 hours, th...

Embodiment 2

[0067] Model verification sample group 16 (external data, newborn 7-day-old SD rats purchased from Liaoning Changsheng Biotechnology Co., Ltd.) to verify the logistic regression model, according to the method in Example 1, and randomly divided into two groups, Feed 8 rats in the Yuanbao maple nerve acid oil group (NA group) and the placebo group (CK group) respectively, and put them into an oxygen chamber simulating a 4500m plateau environment for feeding. After feeding for 30 days, blood and serum were collected.

[0068] According to the method in Example 1, F8: phosphatidylethanolamine-ceramide (d16:1(4E) / 24:1(15Z)), F1: ceramide (d18:1 / 24:1(15Z)), F2: Phosphatidylethanolamine (22:6(4Z,7Z,10Z,13Z,16Z,19Z) / 19:1(9Z)), F3: Sphingomyelin (d17:1 / 24:1), F4: Phosphatidylcholine Base (22:6(4Z,7Z,10Z,13Z,16Z,19Z) / 16:1(9Z)), F5: Phosphatidylcholine (22:6(4Z,7Z,10Z,13Z,16Z,19Z) / 20:2(11Z,14Z)), F6: ceramide (m18:1(4E) / 24:1(15Z)), F7: phosphatidylserine (22:6(4Z,7Z,10Z,13Z,16Z ,19Z) / ...

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Abstract

The invention provides a molecular marker for diagnosing working of nervonic acid supplemented for ischemic hypoxic encephalopathy in a plateau environment and application of the molecular marker. Specifically, the invention relates to the application of the molecular marker, namely phosphatidylethanolamine-ceramide (d16: 1(4E)/24: 1(15Z)) in preparation of an agent for diagnosing the working of nervonic acid supplemented for hypoxic-ischemic encephalopathy in the plateau environment. By detecting the molecular marker, whether acer truncatum bunge nervonic acid oil or similar nervonic acid products are absorbed and converted by an organism or not can be judged, and guiding significance is provided for whether the acer truncatum bunge nervonic acid oil or similar products have effects or not when the acer truncatum bunge nervonic acid oil or similar products are taken.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a molecular marker for diagnosing the onset of nervonic acid supplementation for ischemic-hypoxic encephalopathy in a plateau environment and an application thereof. Background technique [0002] Hypoxic-ischemic encephalopathy refers to diffuse brain tissue damage caused by acute cerebral hypoxia leading to oxygen supply and utilization that cannot meet the metabolic needs of brain tissue, and is more common in newborns. Neonatal hypoxic-ischemic encephalopathy (hypoxie-ischemic encephalopathy, HIE) is an important clinical factor leading to neonatal mortality and neurodevelopmental disorders, and is one of the most common causes of post-neonatal disability. Despite recent advances in obstetric and neonatal care, gestational and perinatal hypoxic-ischemic encephalopathy (HIE) remains a leading cause of neonatal morbidity and mortality worldwide. This ac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/92
CPCG01N33/92G01N2800/7038G01N2800/7019G01N2800/52G01N2800/2871
Inventor 陈显扬宋王婷张珂韩佳睿薛腾
Owner BAO FENG BIOTECH (BEIJING) CO LTD
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