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Endophytic fungus, and application of endophytic fungus in prevention and control of rot disease

A technology for rot disease and rot bacteria, applied in the application field of a method, can solve problems such as increasing the selection pressure of the environment on pathogenic bacteria, environmental pollution, damage to the ecological environment and the like

Active Publication Date: 2021-10-19
ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the extensive use of pesticides not only increases the selection pressure of the environment on pathogenic bacteria, but also causes serious environmental pollution and seriously damages the ecological environment.

Method used

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  • Endophytic fungus, and application of endophytic fungus in prevention and control of rot disease
  • Endophytic fungus, and application of endophytic fungus in prevention and control of rot disease
  • Endophytic fungus, and application of endophytic fungus in prevention and control of rot disease

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Embodiment 1

[0057] Example 1 Isolation and function identification of ZZ1 strains

[0058] 1. ZZ1 strain separation

[0059] The invention is separated from endogenous fungi from the pear branches, wherein a strain is identified by morphological and molecular biological identification as a pecomicillium citrinum, named ZZ1. The separation method is as follows: The pear branches are peeled off, cut into tissue blocks of 0.5 cm × 0.5 cm, disinfect by 75% ethanol, and then disinfect 5 min with 1% NaClo, then washed 3 times with sterile water, The tissue block surface was subjected to water with sterilized filter paper, and tissue block was placed on PDA culture medium (containing 100 mg / L sulfate sulfate), the constant temperature culture tank at 25 ° C, observed once every 24 hours. After the colony is formed, the strain of different colonies is picked up to the new PDA medium, and purified to purify the pure strain is separated, and one strain is named ZZ1.

[0060] Picking the strain ZZ1 si...

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Abstract

The invention discloses an endophytic fungus, and application thereof in prevention and control of rot diseases. According to the invention, the endophytic fungus, namely Penicillium citrinum, isseparated from branches and trunks of pear trees, and the strain number of the Penicillium citrinum is ZZ1. The registration number of the strain in the China General Microbiological Culture Collection Center (CGMCC) is CGMCC No. 22436. The nucleotide sequence of the 18S rDNA contains a DNA molecule as shown in a sequence 1 in a sequence table. Experiments prove that the strain ZZ1 has a good inhibition effect on growth of valsa ceratosperma pear and valsa ceratosperma apple, and the inhibition rate reaches 90% or above. The filtrate of the strain ZZ1 can also significantly inhibit the growth of valsa mali of apple trees and valsa mali of pear trees and the morbidity of fruit and branch rot of pear trees, and the strain ZZ1 has a good application prospect as a biocontrol fungus resource, and can perform safe, effective and environment-friendly biological control on plant rot diseases.

Description

Technical field [0001] The present invention relates to the field of biotechnology, and more particularly to an endogenic fungi and its application in the method of prevention and treatment of rot. Background technique [0002] Rotten disease is also known as rotten skin disease, odde disease, etc. is a fungal disease, which can harm Apple, pear and other important fruit trees, and currently occur in the world. The plants of the tree is rotten, and when the onset is seriously caused, the trendy skin of the tree is necrotic, and even the whole plant is dead. Currently, chemical control is still one of the most effective means of controlling fruit tree rot. However, a large number of pesticides, not only increase the environmental pollution pressure, but also cause serious environmental pollution, and severely destroy the ecological environment. As people's living standards continue to increase, people's quality requirements for environmental and fruit are getting higher and higher...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/36A01P3/00C12R1/80
CPCC12N1/14A01N63/36
Inventor 袁洪波王丽涂洪涛侯珲周增强
Owner ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI