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Production method of recombinant keratin

A production method and keratin technology, applied in the field of keratin production, to achieve good efficacy and high separation purity

Inactive Publication Date: 2021-10-19
海默斯(重庆)医学生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the above-mentioned problems in the prior art, the present invention proposes a production method of recombinant keratin, which solves technical problems such as how to realize industrial production of present recombinant keratin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A method for producing recombinant keratin, comprising the steps of:

[0036] S1. Fermentation preparation: configure the seed culture solution that needs to be sterilized in a sterilizer, sterilize the sterilizer, configure the fermenter salt medium, and clean the fermenter;

[0037] S2. Empty tank sterilization: close all valves of the fermenter before sterilization, turn on the steam generator, and after 15 minutes, click on the sterilization operation on the control panel to perform multi-stage sterilization;

[0038] S3, solid tank sterilization: take out the solution sterilized by the sterilizer, mix and feed, drain, add fermenter salt medium 1, and then sterilize;

[0039] S4, inoculation, the recombinant keratin seed liquid is inoculated in the seed liquid culture medium cooled to room temperature;

[0040] S5. Fermentation: preparation of the fermenter, turn on the stirring switch of the fermenter, set the rotating speed, temperature and flow rate of the ferme...

Embodiment 2

[0061] A method for producing recombinant keratin, comprising the steps of:

[0062] S1. Fermentation preparation: configure the seed culture solution that needs to be sterilized in a sterilizing pot, sterilize in the sterilizing pot, configure the fermenter salt medium 1, and clean the fermenter;

[0063] S2. Empty tank sterilization: close all valves of the fermenter before sterilization, turn on the steam generator, and after 20 minutes, click on the sterilization operation on the control panel to perform multi-stage sterilization;

[0064] S3, solid tank sterilization: take out the solution sterilized by the sterilizer, mix and feed, drain, add fermenter salt medium 1, and then sterilize;

[0065] S4, inoculation, the recombinant keratin seed liquid is inoculated in the seed liquid culture medium cooled to room temperature;

[0066] S5. Fermentation: preparation of the fermenter, turn on the stirring switch of the fermenter, set the rotating speed, temperature and flow ra...

Embodiment 3

[0087] A method for producing recombinant keratin, comprising the steps of:

[0088] S1. Fermentation preparation: configure the seed culture solution that needs to be sterilized in a sterilizing pot, sterilize in the sterilizing pot, configure the fermenter salt medium 1, and clean the fermenter;

[0089] S2. Empty tank sterilization: close all valves of the fermenter before sterilization, turn on the steam generator, and after 25 minutes, click on the sterilization operation on the control panel to perform multi-stage sterilization;

[0090] S3, solid tank sterilization: take out the solution sterilized by the sterilizer, mix and feed, drain, add fermenter salt medium 1, and then sterilize;

[0091] S4, inoculation, the recombinant keratin seed liquid is inoculated in the seed liquid culture medium cooled to room temperature;

[0092] S5. Fermentation: preparation of the fermenter, turn on the stirring switch of the fermenter, set the rotating speed, temperature and flow ra...

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PUM

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Abstract

The invention discloses a production method of recombinant keratin. The production method comprises the following process steps: recombinant keratin fermentation preparation, empty can sterilization, filled can sterilization, recombinant keratin inoculation, recombinant keratin fermentation, sampling detection, bacterium breaking, purification, fine purification, Q column purification, elution and the like. A feasible large-scale production method is provided for fermentation, purification and separation of the recombinant keratin, the integrity of the amino acid structure of the recombinant keratin can be ensured in the whole production process, and the separation purity of the recombinant keratin is relatively high, so the recombinant keratin not only can be applied to more fields of medicines, medical instruments, cosmetics and the like, but also can exert better effects.

Description

technical field [0001] The invention relates to the technical field of keratin production, in particular to a method for producing recombinant keratin. Background technique [0002] Keratin is one of the fibrous structural protein family. It is the main protein that constitutes hair, horns, claws and the outer layer of human skin. Keratin can protect epithelial tissue cells from damage or pressure. With the development of technology, keratin has Highly resistant physical and chemical properties and special biological effects play an important role in maintaining the normal physiological functions of the epidermis. Therefore, keratin is often used in various fields such as feed and food industry, pharmaceuticals, fertilizers, pesticides, cosmetics, etc. The scope is very wide. [0003] In the past, keratin was extracted by hydrolyzing human hair, wool or down with strong acid or alkali, but this step will cause some beneficial amino acids in keratin to be completely destroye...

Claims

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Application Information

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IPC IPC(8): C12P21/02C07K14/47C07K1/14C07K1/16C07K1/34C07K1/36
CPCC07K14/4741
Inventor 查国栋谷加国黎洋洋
Owner 海默斯(重庆)医学生物技术有限公司
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