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Method for evaluating processing quality of glossy privet fruit by microimaging technology

An evaluation method and microscopic imaging technology, which can be used in measurement devices, material analysis and instruments by observing the influence of chemical indicators, and can solve the problems of strong subjectivity, difficulty in evaluating the quality of drug-administered decoction pieces, and high testing costs. Resolve highly subjective effects

Pending Publication Date: 2021-10-22
SHANDONG UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The traditional empirical identification method relies on human sensory evaluation, and there are differences in sensory sensitivity among individuals, which is highly subjective and individual differences are large, especially when the powder is used as a raw material of Chinese patent medicines such as pills, powders, and capsules, it is even more difficult to evaluate The quality of medicinal pieces
Modern instrument detection methods also have the problems of cumbersome sample processing process, complicated instrument operation and high detection cost.

Method used

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  • Method for evaluating processing quality of glossy privet fruit by microimaging technology
  • Method for evaluating processing quality of glossy privet fruit by microimaging technology
  • Method for evaluating processing quality of glossy privet fruit by microimaging technology

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Take Ligustrum lucidum and wine Ligustrum lucidum in Table 1 and Table 2 respectively, crush them, pass through a No. 4 sieve, weigh 2 mg of the sieved powder, put it on a glass slide, and add chloral hydrate test solution dropwise 75 μL, covered with a cover glass, heated in an oven at 90°C for 10 minutes, placed under a microscope after cooling, and took pictures of microscopic characteristics of pericarp epidermal cells, endocarp fibers, and seed coat cells, and microscopic images of Ligustrum lucidum. Feature images such as figure 1 As shown in (a), the microscopic feature picture of Ligustrum lucidum is as follows figure 1 As shown in (b), compared with Ligustrum lucidum, the microscopic characteristic color of wine Ligustrum lucidum was deepened in different degrees.

[0032] Microscope shooting parameters:

[0033] Resolution: The preview resolution is set to 2560 * 1944; capture resolution is 2560 * 1944;

[0034] Exposure control: non-auto exposure (...

Embodiment 2

[0065] Precisely weigh 2 mg of Ligustrum lucidum powder (passed through a No. 4 sieve) and place it on a glass slide, add dropwise 75 μL of chloral hydrate test solution, cover with a cover glass, and heat in an oven at 80°C, 90°C, and 100°C respectively 10min, 20min, microscope field of view and microscopic feature analysis are shown in Table 8 below;

[0066] Table 8 Analysis of the permeation effect of Ligustrum lucidum powder under different baking conditions

[0067] .

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Abstract

The invention discloses a method for evaluating processing quality of glossy privet fruit by a microimaging technology. The method specifically comprises the following steps: respectively weighing glossy privet fruit and wine glossy privet fruit powders on a glass slide, dropwise adding a chloral hydrate test solution, covering with a cover glass, heating in a drying oven, cooling, shooting a microscopic characteristic pattern under a microscope, extracting chromatic values L *, a *, b * and E * ab, determining main difference microscopic characteristics by utilizing partial least squares-discriminant analysis, and establishing a discriminant function and a discriminant rule of the glossy privet fruit and the wine glossy privet fruit through Fisher discriminant analysis; and determining the quality of the glossy privet fruit and the wine glossy privet fruit according to the discrimination function and the discrimination rule. The method for rapidly and conveniently distinguishing the glossy privet fruit from the wine glossy privet fruit is established, can be effectively applied to quality evaluation of Chinese patent medicines such as pills, powder and capsules which take the glossy privet fruit and the wine glossy privet fruit as raw materials, and solves the problems of strong subjectivity, complex sample treatment and high detection cost of the existing distinguishing means of the glossy privet fruit and the wine glossy privet fruit.

Description

technical field [0001] The invention belongs to the technical field of quality analysis of traditional Chinese medicines, and in particular relates to a method for evaluating the processing quality of Ligustrum lucidum by microscopic imaging technology. Background technique [0002] The processing of traditional Chinese medicine is a characteristic of clinical medicine of traditional Chinese medicine. After processing, traditional Chinese medicine can reduce the toxicity and increase the efficacy. The quality of processed traditional Chinese medicine is related to the clinical efficacy of traditional Chinese medicine. Ligustrum lucidum is a plant of Oleaceae Ligustrum lucidum The dry ripe fruit of Ait . has the functions of nourishing the liver and kidney, improving eyesight and black hair. Liquor privet fruit is processed by wine steaming method. Ligustrum lucidum can enhance its liver and kidney function after being steamed with wine, so it is often used as medicine in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/84
CPCG01N21/78G01N21/84G01N2021/786
Inventor 张学兰李慧芬甄臻
Owner SHANDONG UNIV OF TRADITIONAL CHINESE MEDICINE