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Electrochemical measurement device and transducer

A measurement device, electrochemical technology, applied in the direction of measurement devices, electrochemical variables of materials, enzymology/microbiology devices, etc., can solve the problems of cell necrosis and the difficulty in displaying the physiological activity of cell clusters, etc.

Pending Publication Date: 2021-10-22
JAPAN AVIATION ELECTRONICS IND LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This is because: in small cell clusters with a diameter of less than 100 μm, the number of constituting cells is too small, so it is difficult to exhibit the unique physiological activities of the cell cluster; and in large cell clusters with a diameter of more than 600 μm, oxygen will not diffuse to the center of the cell cluster. cells, prone to cell necrosis

Method used

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  • Electrochemical measurement device and transducer
  • Electrochemical measurement device and transducer
  • Electrochemical measurement device and transducer

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Experimental program
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Effect test

no. 1 approach ＞

[0124] Based on the simulation results described above, the first embodiment of the electrochemical measurement device of the present invention has the following configuration. The electrochemical measurement device has: a solution tank 60 ; partitions 10 and 50 ; a wall plate 31 ; and a plurality of working electrodes 21 . All the spacers 10 and 50 , the wall plate 31 and the plurality of working electrodes 21 are fixed on the plane 20 a of the solution tank 60 . The flat surface 20a is the bottom surface of the solution tank 60 that contacts the solution when the solution tank 60 stores the solution, and is also the surface of, for example, a semiconductor chip on which an integrated circuit is formed. The surface of the working electrode 21 that is in contact with the solution is the electrode surface 21a. The spacers 10, 50, the electrode surface 21a, and the wall plate 31 are immersed in the solution during the measurement.

[0125]

[0126] The biological sample 40 h...

no. 2 approach ＞

[0167] Based on the simulation results described above, the second embodiment of the electrochemical measurement device of the present invention has the following configuration. The electrochemical measurement device has: a solution tank 60 ; a plurality of wall plates 32 , 33 ; and a plurality of working electrodes 21 . A plurality of wall plates 32 , 33 and a plurality of working electrodes 21 are all fixed on the plane 20 a of the solution tank 60 . The flat surface 20a is the bottom surface of the solution tank 60 that contacts the solution when the solution tank 60 contains the solution, and is also the surface of, for example, a semiconductor chip on which an integrated circuit is formed. The surface of the working electrode 21 that is in contact with the solution is the electrode surface 21a. The electrode surface 21a and the wall plates 32, 33 are immersed in the solution during the measurement.

[0168]

[0169] About the biological sample 40 and the electrode sur...

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Abstract

Provided is an electrochemical measurement device for measuring a chemical substance generated or consumed by a biological sample in a solution, the electrochemical measurement device comprising a plurality of electrode surfaces, a spacer, and at least one wall plate. The plurality of electrode surfaces, the spacer, and the wall plate are arranged on the same plane. The diameter del of each electrode surface is 80 [mu] m or less. The height of the spacing portion is a predetermined value in the range of h = 21.8 (del + 0.8) / (del + 9.7) + / -5 [mu] m]. The spacer has a structure in which a three-dimensional region closed by the biological sample, the plane, and the spacer is not formed in a state in which the biological sample is in contact with the spacer. The wall plate has the property of being impermeable to solute in the solution and has a height greater than or equal to the height of the spacing part. The two electrode faces are separated by a wall plate.

Description

[0001] This application is a divisional application of an invention patent application with an application date of September 20, 2017, an application number of 201780058155.2, and an invention title of "Electrochemical Measurement Device and Converter". technical field [0002] The present invention relates to an electrochemical measurement device and converter for electrochemical measurement of chemical substances produced or consumed by biological samples. A biological sample is (1) the elements that make up a living thing, or (2) a collection of elements that make up a living thing, or (3) a biologically related substance [a biologically related substance is a chemical substance that exists in a living thing, such as a biopolymer , elements constituting biopolymers, chemical substances required for biological survival], or (4) tangible objects including one or more of (1) to (3). Examples of biological samples include non-biological objects including cells, cell clusters, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48G01N27/327
CPCG01N27/48G01N27/3277C12M1/00C12M1/34G01N33/48728C12M41/32C12M41/46G01N33/5438
Inventor 国方亮太林泰之须田笃史伊野浩介井上久美末永智一
Owner JAPAN AVIATION ELECTRONICS IND LTD