Method for detecting viable bacteria through construction site drinking water electrochemical biosensor and application

Abstract

The invention discloses a method for detecting viable bacteria through a construction site drinking water electrochemical biosensor and application, and belongs to the field of water environment detection. The method comprises the following steps: S1, electrode preparation: removing gold oxide or residue fragments on the surface of a gold disc electrode; S2, thick film manufacturing in electrolyte: applying a constant potential mode to the electrode obtained in the step S1 in the electrolyte to generate the most uniform thick film; S3, thick film washing and carboxyl activation: washing the film, functionalizing the polymer, and placing the polymer in a fresh solution of EDC and NHS for 10 minutes to activate the carboxyl on the film, so that the carboxyl reacts with the selected lectin; S4, EDC / NHS incubation: flushing the membrane with a 10mM PB buffer solution with the pH value of 7.46, placing the membrane in a 1mg / mL agglutinin solution, incubating at room temperature for 2 hours, and then flushing the film with PB; and S5, bacterial concentration determination: determining the bacterial concentration of the approximate culture medium by using a spectrophotometer, diluting by using a PB buffer solution, cleaning cells by using high-pressure PB, and preparing an LB broth and a PB solution by using deionized water.

Description

technical field [0001] The invention relates to the technical field of water environment detection, in particular to a method and application of a construction site drinking water electrochemical biosensor for detecting live bacteria. Background technique [0002] Bacteria detection in water environment is of great significance to human safety and health. Due to disease outbreaks caused by bacterial infections and the emergence of bacterial drug resistance, the demand for sensors is increasing. People are paying more and more attention to the development of faster and more accurate methods for detecting disposable bacteria. Bacterial detection methods are being developed across different fields, from DNA-based assays to surface / affinity interactions. The current platforms such as enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) have their advantages, strengths and specific applications, and they are outstanding in responding to challenges such ...

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Problems solved by technology

While they continue to improve, they are still limited by cost and time due to their inherent multi-step nature

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