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Magnetic particle chemiluminescence detection kit for growth stimulation expression gene 2 protein

A detection kit and gene expression technology, applied in the field of kits, can solve the problems of enzymatic chemiluminescence method with many interference factors, fluorescence immunochromatography method with low sensitivity, enzyme-linked immunoassay method with long detection time, etc. , Strong anti-interference ability and good precision

Pending Publication Date: 2021-11-02
HUNAN YONGHE YANGGUANG SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Enzyme-linked immunoassay takes a long time to detect and has poor repeatability; fluorescence immunochromatography has low sensitivity and is prone to missed detection; enzymatic chemiluminescence has many interference factors and requires a catalyst

Method used

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  • Magnetic particle chemiluminescence detection kit for growth stimulation expression gene 2 protein
  • Magnetic particle chemiluminescence detection kit for growth stimulation expression gene 2 protein
  • Magnetic particle chemiluminescence detection kit for growth stimulation expression gene 2 protein

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preparation example Construction

[0031] In the present invention, the preparation method of the growth-stimulating expression gene 2 protein antibody 1 coated with magnetic beads preferably includes the following steps:

[0032] Wash the magnetic beads with a magnetic bead cleaning solution and resuspend them so that the concentration of the magnetic beads is 5-100 mg / mL; add EDC and NHS to the magnetic bead resuspension solution to activate the magnetic beads; Wash and resuspend the bead cleaning solution, so that the concentration of the activated magnetic beads is 5-100 mg / mL; add growth-stimulating expression gene 2 protein antibody 1 to the resuspended and activated magnetic beads for cross-linking, and use The magnetic bead blocking solution is used for blocking to obtain the growth-stimulating expression gene 2 protein antibody 1 coated with magnetic beads.

[0033] In the present invention, the magnetic beads are firstly washed with a magnetic bead cleaning solution and then resuspended so that the co...

Embodiment 1

[0048] A magnetic particle chemiluminescent immunoassay kit for growth-stimulating expression gene 2 protein, which consists of magnetic particle reagents and acridinium ester reagents;

[0049] The magnetic particle reagent consists of the following components: 4 μg / mL magnetic beads coated growth-stimulating expression gene 2 protein antibody 1, PBS buffer 0.01M, newborn bovine serum 250mL / L, Proclin3000.5 mL / L, glycerol 250mL / L , Tween 200.1mL / L and BSA2g / L;

[0050] The acridinium ester reagent consists of the following components: 10 μg / mL acridinium ester-labeled growth-stimulating expression gene 2 protein antibody 2, PBS buffer 0.01 M, glycerol 300 mL / L and BSA 10 g / L.

[0051] Preparation:

[0052] 1 Preparation method of growth-stimulating expression gene 2 protein antibody 1 coated with magnetic beads

[0053] 1) Add 200 μL of magnetic beads into a 2 mL centrifuge tube, magnetically separate for 3 minutes, and then remove the supernatant. Among them, the magnetic ...

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Abstract

The invention provides a magnetic particle chemiluminescence detection kit for growth stimulation expression gene 2 protein, and belongs to the technical field of kits. The kit provided by the invention comprises a magnetic particle reagent and an acridinium ester reagent, the magnetic particle reagent comprises an ST2 antibody 1 coated with magnetic beads; the ST2 antibody 1 is purchased from Beijing holmes Biotechnology Co., Ltd., and the product number is HM148A-1148E; the acridinium ester reagent comprises an ST2 antibody 2 marked by acridinium ester; the ST2 antibody 2 is purchased from Beijing holmes Biotechnology Co., Ltd., and the product number of the ST2 antibody 2 is HM148A-1148F; the particle size of the magnetic beads is 1-3 microns, and carboxyl active groups are arranged on the surfaces of the magnetic beads. The magnetic particle chemiluminescence immunoassay kit for the growth stimulation expression gene 2 protein provided by the invention is high in sensitivity, good in specificity, high in precision, short in reaction time and simple to operate, and does not need to add a catalyst.

Description

technical field [0001] The invention belongs to the technical field of kits, and in particular relates to a magnetic particle chemiluminescence detection kit for growth stimulation expression gene 2 protein. Background technique [0002] Growth-stimulating expression gene 2 protein (ST2) is a member of the interleukin receptor family, consisting of 328 amino acids, with 3 immunoglobulin domains and 9 glycosylation sites, which can be expressed by cardiomyocytes during myocardial stress . The 2013 American ACC / AHA / HFSA heart failure guidelines pointed out that ST2 is a marker of myocardial fibrosis, which can predict the admission and death probability of heart failure patients. In 2017, ST2 was included in the "Guidelines for Clinical Practice in the Emergency Department of Acute Heart Failure in China". In 2020, ST2 was included in the "2020 Chinese Expert Consensus on Heart Failure Biomarkers". At present, clinical or laboratory methods for ST2 detection include enzyme-...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/543G01N33/532
CPCG01N33/6869G01N33/54326G01N33/532G01N2333/7155G01N2800/325Y02A50/30
Inventor 刘健王德健沈林陈汝彬孙长贵聂勇黄博文雷铮宇孔佳能赵彦
Owner HUNAN YONGHE YANGGUANG SCI & TECH
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