Method for improving sporulation of piriformospora indica

A technology of piriformis sp., culture method, applied in the direction of using spores, methods based on microorganisms, biochemical equipment and methods, etc., which can solve the problems of complicated medium composition, unsuitable long fermentation period, and extraction of cold shock protein cumbersome and other problems, to achieve the effect of shortening the culture period, increasing the spore production, and benefiting industrial production

Active Publication Date: 2021-11-05
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI +3
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above method of fermentation culture requires the extraction of cold shock protein, which is cumbersome, and the liquid seed liquid used for inoculatio

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for improving sporulation of piriformospora indica
  • Method for improving sporulation of piriformospora indica
  • Method for improving sporulation of piriformospora indica

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The optimization (screening of potato flour and glucose proportioning) of embodiment 1 Indian piriformis fermentation medium

[0039] Experimental materials: Pyromorpha indica strain, hemocytometer, centrifuge, puncher, gauze, shaker, vortex oscillator, oven, ultrasonic instrument, 1mol / L NaOH standard solution, 1mol / L HCl standard solution, PDA medium, PDB medium, liquid seed medium.

[0040] Medium for 1% whole potato powder: Weigh 10 g of whole potato powder, adjust the volume to 1000 ml with water, and adjust the pH to 7.

[0041] Medium of 1% whole potato flour + 1% glucose: Weigh 10g of whole potato flour and 10g of glucose, dilute to 1000ml with water, and adjust the pH to 7.

[0042] Medium of 1% whole potato flour + 2% glucose: Weigh 10g of whole potato flour and 20g of glucose, dilute to 1000ml with water, and adjust the pH to 7.

[0043] Medium of 2% whole potato powder + 1% glucose: Weigh 20 g of whole potato powder and 10 g of glucose, dilute to 1000 ml w...

Embodiment 2

[0057] The optimization of embodiment 2 Indian piriformis fermentation culture conditions

[0058] Experimental materials: 50ml homogenizer, shaker, 500ml Erlenmeyer flask, PDA medium, PDB medium, liquid seed medium.

[0059] Activation of Piriformospora indica and acquisition of seed solution: activate (Piriformospora indica) ACCC 39199 on PDA medium, culture at 28°C in the dark, and cultivate for 15 days to take a 5mm fungus cake with a punch Put the liquid seed culture medium, and cultivate it in the dark at 28°C and 200rpm for 7 days to obtain the seed liquid; it can be stored in a constant temperature refrigerator at 4°C for future use.

[0060] Optimization indicators:

[0061] Measure spores: Add 1 mL of Tween to 100 mL of culture broth, vortex, grind in a blender, and sonicate for 5 min to separate spores and hyphae. After isolation, spores were counted using a hemocytometer.

[0062] Determination of mycelia production: after fermentation, centrifuge the mixture in...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a method for improving sporulation of piriformospora indica. According to a piriformospora indica culture method, an adopted fermentation culture medium is composed of or prepared from dried potato powder, glucose and water, the content of the dried potato powder in the fermentation culture medium is 10g/L-20g/L, and the content of the glucose in the fermentation culture medium is 10g/L-20g/L. According to the method, piriformospora indica is cultured in the fermentation culture medium composed of the dried potato powder, the glucose and the water, the culture period can be shortened, the spore yield can be increased, the production cost is greatly reduced, and industrial production is facilitated.

Description

technical field [0001] The invention relates to a method for improving spore production of Piriformis indica. Background technique [0002] Piriformospora indica is a fungus that can colonize the rhizosphere of plants and has beneficial effects on plant growth and development. It has been reported that the fungus can promote the growth of 24 species of host plants in 12 families, and can have symbiotic effects with 200 species of plants, including agriculture, horticulture, important economic crops and medicinal plants. It is known that Pyriformis indica acting on corn can improve the drought resistance of corn and increase the yield of maize; co-cultivation of Pyromorpha indica and pakchoi can significantly increase the biomass of pakchoi, promote the growth of the main root of pakchoi, increase the number of lateral roots and Length lengthening, root hairs, etc.; when Piriformis indica and tobacco are co-cultivated, the protein content in tobacco leaves can be significant...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/14C12N3/00C12R1/645
CPCC12N1/14C12N3/00
Inventor 张桂山苗少华王华付易可可胡四化龚旗孙颖娇
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap