Application of miR-6883-3p in preparation of anti-liver cancer or liver cancer prognosis evaluation product

A liver cancer prognosis and anti-liver cancer technology, applied in the field of biomedicine, can solve problems such as complex mechanism and no effective treatment method, achieve the effect of inhibiting proliferation, improving clinical treatment effect, good clinical development and application value

Pending Publication Date: 2021-11-05
GUANGZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The course of liver cancer progresses rapidly, the mechanism is complex, and there is currently no effective treatment

Method used

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  • Application of miR-6883-3p in preparation of anti-liver cancer or liver cancer prognosis evaluation product
  • Application of miR-6883-3p in preparation of anti-liver cancer or liver cancer prognosis evaluation product
  • Application of miR-6883-3p in preparation of anti-liver cancer or liver cancer prognosis evaluation product

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Experimental program
Comparison scheme
Effect test

Embodiment

[0034] The present invention provides the application of miR-6883-3p in the preparation of anti-liver cancer or liver cancer prognosis assessment products, the sequence of the miR-6883-3p is:

[0035] 5'-UUCCCUAUCUCACUCUCCUCAG-3' (as shown in SEQ ID NO.1).

[0036] The present invention also provides a small-molecule drug against liver cancer, the small-molecule drug includes an effective dose of miR-6883-3p or a biologically active functional fragment or variant thereof, which may be, but not limited to, contain miR-6883-3p A recombinant vector of 3p nucleotide sequence or a recombinant virus or one of recombinant virus vectors.

[0037] The small molecule drug includes a pharmaceutically acceptable small molecule compound, carrier or auxiliary material, and the small molecule compound, carrier or auxiliary material can be, but not limited to, chitosan, cholesterol, liposome, cyclodextrin, One or more of microspheres or microcapsules.

[0038] The administration method of t...

experiment example 1

[0051] The clinically collected pairs of liver cancer and paracancerous tissue specimens used in this experiment were all freshly isolated tumor tissues from the Cancer Hospital Affiliated to Guangzhou Medical University. After cutting into small tissue pieces, they were frozen at -80°C for later use. The expression level of miR-6883-3p was determined by taking liver cancer tissue and adjacent tissue as detection objects.

[0052] 1. miRNA extraction:

[0053] After grinding the tissue with liquid nitrogen, add 1mL RNAiso for Small RNA (miRNA extraction reagent) and let it stand at room temperature for 10min; pipette the cells until there are no cells at the bottom of the dish, add the cell lysate to an RNase-free EP tube, add 200μL chloroform, and place Shake on a shaker for 15 seconds, then let stand on ice for 5 minutes, then place in a centrifuge, and centrifuge at 12,000 rpm for 10 minutes at 4°C; carefully absorb the upper RNA aqueous phase and transfer to another new EP...

experiment example 2

[0073] In order to clarify the biological function of miR-6883-3p in liver cancer cells, miR-6883-3p and its inhibitor (inhibitor for short) in the present invention were used in this experimental example, and the liver cancer cell lines CRL-8024 and Huh7 were used as experiments In this model, miR-6883-3p and inhibitor were transfected into liver cancer cells to up-regulate or down-regulate the level of miR-6883-3p in the cells, so as to carry out the experiment of miR-6883-3p inhibiting the malignant phenotype of liver cancer cells, miR-6883-3p and The inhibitor was transferred into liver cancer cells according to the amount recommended in the instruction manual of the transfection reagent. After 48 hours, the total miRNAs were extracted according to the method provided in Experimental Example 1, and the expression level of miR-6883-3p was detected by reverse transcription and fluorescent quantitative PCR.

[0074] MTS cell proliferation detection kit was used to measure the ...

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Abstract

The invention relates to the technical field of biomedicine, discloses an application of miR-6883-3p in preparation of an anti-liver cancer product or a liver cancer prognosis evaluation product, and particularly relates to an application of miR-6883-3p in preparation of an anti-liver cancer drug or a liver cancer prognosis evaluation kit. The miR-6883-3p targeted gene regulation network provided by the invention is closely related to malignant phenotype and poor prognosis of tumors, overexpression of miR-6883-3p can effectively inhibit proliferation and migration of liver cancer cells, promote apoptosis of the liver cancer cells and reverse the differentiation state of the liver cancer cells, and the miR-6883-3p is low in expression in liver cancer tissues, so that the miR-6883-3p can be used for preparing antitumor drugs or detection kits and has good application prospects.The method has potential application value in treatment or prognosis evaluation of hepatocellular carcinoma.

Description

technical field [0001] The present invention relates to the field of biomedical technology, and more specifically relates to the application of miR-6883-3p in the preparation of products for anti-hepatic cancer or liver cancer prognosis assessment. Background technique [0002] Hepatocellular carcinoma (liver cancer for short) is a malignant tumor with high incidence in Asia, and its mortality rate ranks fourth among malignant tumors. The five-year survival rate is only 18%. It is insensitive to radiotherapy and chemotherapy and has a poor prognosis. The course of liver cancer progresses rapidly, the mechanism is complicated, and there is no effective treatment at present. Finding specific molecular targets for liver cancer is an urgent problem to be solved. [0003] microRNAs (miRNAs) are a class of non-coding small molecule RNAs encoded by endogenous genes with a length of about 18-22 nucleotides. They recognize specific target molecules through the principle of complemen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/11A61K31/7105A61P35/00
CPCC12Q1/6886A61K31/7105A61P35/00C12Q2600/178C12Q2600/158C12Q2600/118
Inventor 刘珊珊黄丽成炜张晓锋陈晓刚马宁芳
Owner GUANGZHOU MEDICAL UNIV
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