Visual biological membrane culture reactor and in-situ observation method

Abstract

The invention discloses a visual biological membrane culture reactor and an in-situ observation method; the reactor comprises a reactor main body, a reference electrode and a counter electrode; the reference electrode and the counter electrode penetrate through the side wall of the reactor main body and extend into a reaction chamber; the reactor main body comprises a body, a sealing cover, a clamping part, a light-transmitting bottom plate and a conductive probe; the body is provided with a through hollow cavity; the sealing cover is sealed and detachably connected with a port at one end of the hollow cavity; a transparent skylight is arranged in the centre of the sealing cover; the clamping part protrudes outwards relative to the bottom surface of the body; a through hole coaxially penetrating through the hollow cavity is formed in the clamping part; the light-transmitting bottom plate is sealed and detachably connected with a port at the other end of the hollow cavity or the through hole of the clamping part; the inner surface of the light-transmitting bottom plate is a conductive surface or a conductive glass sheet is arranged on the inner side of the light-transmitting bottom plate; one end of the conductive probe extends into the reaction chamber and is in clutch connection with the conductive surface or the conductive glass sheet; and the other end of the conductive probe extends out of the body. According to the invention, the bottlenecks of in-situ sampling, in-situ dyeing and in-situ confocal observation and detection are overcome.

Description

technical field [0001] The invention relates to the technical field of microbial electrochemistry, in particular to a visualized biofilm cultivation reactor and a method for cultivating and observing biofilm using the reactor. Background technique [0002] At present, biofilm has become a research hotspot in the fields of medicine, food and environment. However, until now, biofilm is still a "black box", and the material circulation, community structure and function in the membrane have yet to be explored. In 2004, Logan et al. (Liu H, Ramnarayanan R, Logan BE. Production of electricity during wastewater treatment using a single chamber microbial fuel cell [J]. Environ Sci Technol, 2004, 38 (7): 2281-2285) found that from wastewater The electron transfer efficiency of the enriched biofilm adsorbed on the solid electrode is hundreds of times that of planktonic microorganisms. This type of biofilm formed by microorganisms that can exchange electrons with conductive materials...

AI Technical Summary

Problems solved by technology

[0006] The invention provides a visual reactor to a biofilm reactor, which overcomes the bottlenecks that the traditional biofilm reactor cannot realize real-time visual observation and the inconvenient in-situ sampling, in-situ staining, and in-situ confocal observation and detection of existing visual reactors. Mechanistic studies during biofilm reactions provide strong support

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