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Clerodendrum japonicum extract and application of clerodendrum japonicum extract in preparation of anti-inflammatory composition

A technology of extract and fenugreek, applied in the field of natural medicine, can solve the problem of unclear active ingredients in specific links, and achieve the effect of strong anti-inflammatory ability

Inactive Publication Date: 2021-11-16
GUANGXI INT ZHUANG MEDICINE HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the previous research work, it was found that the part of ethyl acetate of eucalyptus became the most effective part of anti-inflammatory activity. However, the specific links and targets of its anti-inflammatory effect and the main active ingredients are still unclear

Method used

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  • Clerodendrum japonicum extract and application of clerodendrum japonicum extract in preparation of anti-inflammatory composition
  • Clerodendrum japonicum extract and application of clerodendrum japonicum extract in preparation of anti-inflammatory composition

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Experimental program
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Effect test

Embodiment 1

[0023] The preparation of the ethyl acetate part of embodiment 1 eucalyptus

[0024] 1. Medicinal materials

[0025] The medicinal material was collected from Wuming District, Nanning City, Guangxi. It was identified as Clerodendrum japonicum (Thunb.) Sweet., a plant of the genus Verbenaceae, by Tang Chunli, deputy director of the Pharmacy Department of the First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine. strain.

[0026] 2. Preparation method

[0027] Soak 11.5Kg of the medicinal material of Tungia chinensis with 12 times the amount of 80% ethanol for 24 hours, then reflux extraction for 1.5 hours, then filter, repeat the extraction 3 times, add 10 times the amount of 60% ethanol to reflux extraction twice, each time for 1.5 hours, and combine The filtrate was concentrated until there was no ethanol smell, and 1178.75g of ethanol extract was obtained. After adding 1000ml of pure water to suspend, add petroleum ether with a volume ratio of 1:1...

Embodiment 2

[0029] Embodiment 2 The preparation of dichloromethane-methanol fraction

[0030] Take 150g of the extract from the ethyl acetate part of the tung tree and add 350ml of methanol to dissolve it, mix it with 120g of column chromatography silica gel (200-300 mesh) to mix the sample, dry it and load the sample, the column specification is 6cm in diameter and 60cm in height. Respectively dichloromethane-methanol (70:1), dichloromethane-methanol (50:1), dichloromethane-methanol (30:1), dichloromethane-methanol (20:1), dichloromethane- Methanol (10:1) gradient elution, the amount of each gradient is 10 times the column volume, the elution fractions of each gradient were collected, concentrated and freeze-dried. Get the dry ointment of each part, seal it and keep it for later use.

[0031] Take 0.15g of different fractions of dry paste and weigh them accurately, add 0.1% DMSO to dissolve it ultrasonically, then dilute to 3mg / mL with RPMI1640 incomplete culture medium, pass through a ...

Embodiment 3

[0032] Embodiment 3 The preparation of chloroform-methanol fraction

[0033] Take 150g of the extract from the ethyl acetate part of the tung tree and add 350ml of methanol to dissolve it, mix it with 120g of column chromatography silica gel (200-300 mesh) to mix the sample, dry it and load the sample, the column specification is 6cm in diameter and 60cm in height. Use chloroform-methanol (70:1), chloroform-methanol (50:1), chloroform-methanol (30:1), chloroform-methanol (10:1) gradient elution, and the amount of each gradient is 10 times the column volume , each gradient elution fraction was collected separately, concentrated and freeze-dried. Get the dry ointment of each part, seal it and keep it for later use.

[0034] Take 0.15g of different fractions of dry paste and weigh them accurately, add 0.1% DMSO to dissolve it ultrasonically, then dilute to 3mg / mL with RPMI1640 incomplete culture medium, pass through a sterile filter membrane, and then dilute to the required conc...

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Abstract

The invention relates to the field of natural medicines, in particular to a clerodendrum japonicum extract and application thereof in preparation of an anti-inflammatory composition. The clerodendrum japonicum extract is prepared by the following steps: extracting clerodendrum japonicum with ethanol, sequentially extracting with petroleum ether and ethyl acetate, and eluting the ethyl acetate part with a mixed solvent of a weak polar solvent and a strong polar solvent in a certain proportion. The anti-inflammatory mechanism of the ethyl acetate part of the clerodendrum japonicum and the weak polar solvent-strong polar solvent part with different gradients of the ethyl acetate part of the clerodendrum japonicum plays a role in inhibiting the generation of NO, TNF-a, IL-12, IL-6 and IL-1beta inflammatory factors.

Description

technical field [0001] The invention relates to the field of natural medicines, in particular to an extract from Tunga tungii and its application in the preparation of anti-inflammatory compositions. Background technique [0002] Thunb. is Clerodendrum japonicum (Thunb.) Sweet., a plant of the genus Verbenaceae, also known as Red Dragon Boat, Thunb. Red, etc., "Gepeng Quilt" (Guangxi Zhuang language), produced in Guangxi, Guizhou and Yunnan , is a traditional folk medicine. Tung tree mainly contains flavonoids, phenolic acids, tannins and volatile oils. The whole plant can be used as medicine. It is sweet in taste and cool in nature. It has anti-inflammatory, anti-oxidation and anti-tumor pharmacological effects. Stasis, carbuncle sores; clinically used for the treatment of lung-heat cough, hot stranguria, and dysuria. In the previous research work, it was found that the part of ethyl acetate of E. japonica became the most effective part of anti-inflammatory activity. Howe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/855A61P29/00
CPCA61K36/855A61P29/00A61K2236/333A61K2236/39A61K2236/53A61K2236/51A61K2236/55
Inventor 秦祖杰魏江存唐云丽覃丽萍马秀梅王成龙蔡文威丘琴马艳郑玲谭雨坪
Owner GUANGXI INT ZHUANG MEDICINE HOSPITAL