Exosome separation micro-fluidic chip and preparation method thereof

Abstract

The invention discloses an exosome separation micro-fluidic chip and a preparation method thereof, the exosome separation micro-fluidic chip comprises an exosome micro-fluidic chip, the exosome micro-fluidic chip is formed by bonding a cover glass base and a PDMS chip, the exosome micro-fluidic chip is internally provided with a plurality of groups of separation capture units, each separation capture unit is composed of two left-right symmetrical quarter arc structures, the distance between the two arc structures of the separation capture unit is smaller than the diameter of a microsphere loaded with the exosome, and the height of the exosome micro-fluidic chip is 30-50 [mu] m; the size of the separation capture unit is greatly reduced through the arrangement of two quarter ring groups of the separation capture unit, capture units in a chip with the same area are increased, the flux is improved, the capture rate is increased, the structure is simple, separation capture does not need external force, separation can be realized only through sample introduction, the operation is simple, rapid and convenient, meanwhile, capturing can be achieved through forward liquid passing of the chip, releasing can be achieved through reverse liquid passing of the chip, and bidirectional operation can be achieved.

Description

technical field [0001] The invention relates to the technical field of exosome separation, in particular to a microfluidic chip for exosome separation and a preparation method thereof. Background technique [0002] Exosomes are tiny vesicles coated with phospholipid bilayers with a size of 30-150nm. They are a type of extracellular vesicles and can be secreted by various types of cells. Information, including DNA, RNA, protein, etc., can reflect many information of parental cells. At the same time, after exosomes are secreted, they are abundant in circulating body fluids, and their concentration in blood is significantly higher than that of circulating tumor cells. Therefore, tumor Cell-derived exosomes can reflect tumor status and are ideal tumor biomarkers; [0003] Because the size of exosomes is on the nanometer scale, it is difficult to separate them with high purity from the complex body fluid environment with existing technologies. At present, the main separation met...

AI Technical Summary

Problems solved by technology

[0003] Because the size of exosomes is on the nanometer scale, it is difficult to separate them with high purity from the complex body fluid environment with existing technologies. At present, the main separation method of exosomes is ultracentrifugation, but it takes a long time and the equipment is expensive. Moreover, the isolated exosomes are easily contaminated by other vesicles and macromolecular proteins. Commercial exosome isolation kits mainly use polymers or magnetic beads to separate exosomes, but when exosomes are precipitated, foreign proteins are also precipitated, resulting in The results are misleading, and the traditional microfluidic chip is mostly formed by the lateral flow force or protein affinity capture formed by the internal size of the chip. The separation of the lateral flow principle is likely to cause chip blockage, and the protein affinity capture is easily affected by the specific antibody immobilization efficiency. Moreover, the separated exosomes in the chip are not easy to observe. Therefore, the present invention proposes a microfluidic chip for separating exosomes and a preparation method to solve the problems existing in the prior art.

Images