Method for extracting hesperidin from citrus to prepare hesperetin

A technology of hesperidin and hesperetin, applied in the field of hesperetin, can solve the problems of waste, complicated process conditions, increased cost of hesperetin, etc., and achieve the effects of reducing intermediate loss and shortening production cycle

Inactive Publication Date: 2021-11-23
广州健顺生物医药研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Hesperetin is a kind of natural flavonoids, which are widely found in fruits and other substances. It is widely used in medicine and food. Hesperetin is mainly prepared by hydrolysis of hesperidin, while citrus is rich in orange peel

Method used

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  • Method for extracting hesperidin from citrus to prepare hesperetin
  • Method for extracting hesperidin from citrus to prepare hesperetin
  • Method for extracting hesperidin from citrus to prepare hesperetin

Examples

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preparation example Construction

[0042] a) Preparation of reference substance solution: Accurately weigh 0.01g hesperetin reference substance, accurate to 0.0001g, dissolve it with mobile phase, transfer it into a 100mL volumetric flask, add mobile phase to constant volume to 100mL, and filter the obtained solution with a 0.45μm filter membrane Filter and use the filtrate for later use.

[0043] b) Preparation of sample solution: Accurately weigh 0.01g sample, accurate to 0.0001g, dissolve it with mobile phase, transfer it into a 100mL volumetric flask, add mobile phase to make the volume to 100mL, filter the obtained solution with a 0.45μm filter membrane, and filter the filtrate spare.

[0044] c) Determination: Under the above-mentioned chromatographic conditions, measure the reference substance solution and the sample solution respectively, and record the main peak area.

[0045] d) Calculation of results: mass fraction w of hesperetin content 1 Calculated according to the following formula

[0046] ...

Embodiment 1

[0061] Example 1: Grind the dried citrus, pass through a 100-mesh sieve, weigh 100kg of powder, add solvent water, the ratio of solid to liquid (w / v) is 1:10, adjust the pH of the solution to 11.0, and place it in a homemade Extract in the ultrasonic countercurrent extraction device for 120min, filter, and collect the extract; after extracting twice, combine the extract, concentrate the combined extract to 1 / 3 of the volume of the original extract, adjust the concentrated extract Liquid pH to 3.0, settling for 8 hours, filtering to collect the precipitate, washing the precipitate with pure water to neutrality and drying into powder, weighing to obtain citrus crude extract, adding water to the weighed citrus crude extract, the ratio of solid to liquid (w / v) is 1:40, add amylase (1:20), pectinase (1:20), protease (1:20), rhamnosidase (1:30), β-glucoside Enzyme (1:30) for hydrolysis reaction, the reaction conditions are: temperature 50°C, pH 5.5, time 5h, after the reaction is co...

Embodiment 2

[0062] Example 2: Grind the dried citrus, pass through a 100-mesh sieve, weigh 100kg of powder, add solvent water, the ratio of solid to liquid (w / v) is 1:15, adjust the pH of the solution to 11.0, and place it in a homemade Extract in an ultrasonic countercurrent extraction device for 120min, filter, collect the extract, extract twice, combine the extract, concentrate the combined extract to 1 / 4 of the volume of the original extract, and adjust the concentrated extract Liquid pH to 3.0, settling for 8 hours, filtering to collect the precipitate, washing the precipitate with pure water to neutrality and drying into powder, weighing to obtain citrus crude extract, adding water to the weighed citrus crude extract, the ratio of solid to liquid (w / v) is 1:50, add amylase (1:30), pectinase (1:30), protease (1:30), rhamnosidase (1:40), β-glucoside Enzyme (1:40) for hydrolysis reaction, the reaction conditions are: temperature 50°C, pH 5.5, time 5h, after the reaction is completed, l...

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Abstract

The invention relates to the technical field of extraction of plant effective components, in particular to a method for extracting hesperidin from citrus to prepare hesperetin, which comprises the following specific steps: S1, drying citrus, crushing, filtering with a screen, weighing 100 kg of powder, adding a solvent for extraction, adjusting the pH value to be alkaline, putting the powder into a self-made ultrasonic countercurrent extraction device for extraction for 120 minutes, filtering, collecting an extracting solution, extracting twice, combining the extracting solution, concentrating the combined extracting solution until the volume is 1/(2-4) of the volume of the original extracting solution, filtering, adjusting the pH value to 3-4, standing and precipitating for 8 hours, filtering and collecting a precipitate, washing the precipitate with pure water until the precipitate is neutral, drying into powder, and weighing to obtain the citrus crude extract. According to the invention, the hesperetin is produced by designing and utilizing the waste citrus. Compared with the traditional process for preparing hesperetin by taking hesperidin as a raw material, the method has the advantages that the intermediate loss is reduced, the purification and drying links of the hesperidin are reduced, and the production period is greatly shortened.

Description

technical field [0001] The invention relates to the technical field of extraction of effective plant components, in particular to a method for preparing hesperetin by extracting hesperidin from citrus. Background technique [0002] Hesperetin is a kind of natural flavonoids, which are widely found in fruits and other substances. It is widely used in medicine and food. Hesperetin is mainly prepared by hydrolysis of hesperidin, while citrus is rich in orange peel Glycosides, and my country's citrus is rich in resources, which will cause a lot of waste every year, and the complex conditions of the existing hesperetin preparation process have caused an increase in the cost of the hesperetin produced. [0003] In summary, the present invention solves the existing problems by designing a method for preparing hesperetin from oranges by extracting hesperidin. Contents of the invention [0004] The object of the present invention is to provide a kind of method that extracts hesperi...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/14G01N30/30G01N30/32G01N30/34G01N30/74G01N30/86
Inventor 张达成钟国坚刘园吴茂忠
Owner 广州健顺生物医药研究院有限公司
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