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Carbon dots, preparation method thereof and application of carbon dots in preparation of mitochondria-targeting fluorescent probe

A technology of carbon dots and fluorescent carbon dots, which is applied in the direction of fluorescence/phosphorescence, chemical instruments and methods, nano-carbon, etc., can solve the problems of not having the property of targeting mitochondria, and achieve the effect of stable fluorescence properties and high fluorescence quantum yield

Pending Publication Date: 2021-11-26
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Nevertheless, most carbon dots prepared so far do not have the property of targeting mitochondria

Method used

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  • Carbon dots, preparation method thereof and application of carbon dots in preparation of mitochondria-targeting fluorescent probe
  • Carbon dots, preparation method thereof and application of carbon dots in preparation of mitochondria-targeting fluorescent probe
  • Carbon dots, preparation method thereof and application of carbon dots in preparation of mitochondria-targeting fluorescent probe

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A method for preparing carbon dots is provided, comprising the steps of:

[0039] Step 1: Weigh 0.523g of tartaric acid and 0.476g of diethylaminophenol respectively for later use.

[0040] Step 2: Put the weighed tartaric acid and diethylaminophenol into 30mL ultrapure water (the water resistivity is 18.4MΩ·cm -1 ) in a beaker, and stir to mix.

[0041] Step 3: Put the above-mentioned mixed solution into a 50mL polytetrafluoroethylene reactor, and heat the reactor in an oven at a constant temperature of 180°C for 24 hours, then lower the temperature to 25°C.

[0042] Step 4: Put the brown solution obtained from the reaction into a 500Da dialysis bag, put it into a beaker filled with 2L ultrapure water for dialysis, and change the water every 4 hours to remove impurities, and finally obtain wine red carbon Dot the solution.

Embodiment 2

[0044] A method for preparing carbon dots is provided, comprising the steps of:

[0045] Step 1: Weigh 0.1g of tartaric acid and 0.1g of diethylaminophenol respectively for later use.

[0046] Step 2: Put the weighed tartaric acid and diethylaminophenol into 30mL ultrapure water (the water resistivity is 18.4MΩ·cm -1 ) in a beaker, and stir to mix.

[0047] Step 3: Put the above mixed solution into a 50mL polytetrafluoroethylene reactor, and heat the reactor in an oven at a constant temperature of 160°C for 6 hours, then lower the temperature to 25°C.

[0048] Step 4: Put the brown solution obtained from the reaction into a 500Da dialysis bag, put it into a beaker filled with 2L ultrapure water for dialysis, and change the water every 4 hours to remove impurities, and finally obtain wine red carbon Dot the solution.

Embodiment 3

[0050] A method for preparing carbon dots is provided, comprising the steps of:

[0051] Step 1: Weigh 1g of tartaric acid and 1g of diethylaminophenol respectively for future use.

[0052] Step 2: Put the weighed tartaric acid and diethylaminophenol into 30mL ultrapure water (the water resistivity is 18.4MΩ·cm -1 ) in a beaker, and stir to mix.

[0053] Step 3: Put the above-mentioned mixed solution into a 50mL polytetrafluoroethylene reactor, and heat the reactor in an oven at a constant temperature of 200°C for 48 hours, then lower the temperature to 25°C.

[0054] Step 4: Put the brown solution obtained from the reaction into a 500Da dialysis bag, put it into a beaker filled with 2L ultrapure water for dialysis, and change the water every 4 hours to remove impurities, and finally obtain wine red carbon Dot the solution.

[0055] The carbon dot prepared in Example 1 is characterized, and the specific characterization results are as follows:

[0056] 1. Characterize the ...

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Abstract

The invention discloses a carbon dot, a preparation method thereof and application of the carbon dot in preparation of a mitochondrial targeting fluorescent probe; wherein the preparation method of the carbon dot comprises the following steps: respectively weighing 0.1-1g of tartaric acid and 0.1-1g of diethylaminophenol for later use; putting weighed tartaric acid and diethylaminophenol into ultrapure water, and uniformly stirring and mixing the components; filling the uniformly mixed solution into a reaction kettle, heating the reaction kettle at 160-200 DEG C for 6-48 hours, and cooling the reactants to room temperature; and filling a dialysis bag with a brown solution obtained by the reaction, dialyzing the brown solution in ultrapure water, and changing water once at set intervals to remove impurities, thereby finally obtaining the carbon dot solution in the wine-red color. The carbon dots prepared by the hydrothermal method in one step have high fluorescence quantum yield and stable fluorescence property, and are non-toxic; wherein cell imaging experiments show that the prepared carbon dots can be used as mitochondrial fluorescent probes, and have potential application in the fields of disease diagnosis, cell imaging, molecular detection and the like.

Description

technical field [0001] The invention belongs to the technical field of fluorescent carbon dots, and in particular relates to a carbon dot, a preparation method thereof and an application in preparing a fluorescent probe targeting mitochondria. Background technique [0002] Mitochondria are important organelles of mammalian cells and the power station of cells. In addition to providing cellular energy, they are also involved in many important cellular metabolic processes, such as cell growth, necrosis, and apoptosis. Interestingly, mitochondria exhibit dynamic properties including fission, fusion, and trafficking during these biological processes. This dynamic change of mitochondria plays a fundamental role in maintaining their shape and function. Therefore, it is of great interest to visualize and monitor the dynamics of mitochondria in living cells. So far, various probes have been applied to mitochondrial imaging, including fluorescent proteins and small organic molecul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/65B82Y20/00B82Y40/00C01B32/15G01N21/64
CPCC09K11/65C09K11/0883B82Y20/00B82Y40/00C01B32/15G01N21/6428
Inventor 李灏郭嘉庆刘爱坤曾禹天蔡浩杰宋军屈军乐
Owner SHENZHEN UNIV
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