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Heavy metal tolerant fusarium X-2-5 and application thereof

A heavy metal and fusarium technology, applied in the field of environmental governance, can solve the problems of less research results and other problems, and achieve the effect of restoring vegetation coverage, good marketing value, and reducing content

Active Publication Date: 2021-11-26
湖南省环境保护科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Therefore, looking for endophytic bacteria with strong tolerance to heavy metals from plants in heavy metal polluted areas has a strong purpose and feasibility, but there are few reports on the specific research results of this type of endophytic bacteria

Method used

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  • Heavy metal tolerant fusarium X-2-5 and application thereof
  • Heavy metal tolerant fusarium X-2-5 and application thereof
  • Heavy metal tolerant fusarium X-2-5 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034]Potato liquid medium was configured, and the prepared medium was placed in a 200mL Erlenmeyer flask, sealed and sterilized. After cooling, inoculate Fusarium X-2-5 under aseptic conditions, and cultivate it at 25°C for 20 to 30 days in an indoor environment. After the mycelium is covered with liquid medium, stir it evenly and dilute it by 2 times. Seed soaking of land seeds, after the seeds absorb water and swell, they are placed in a petri dish to accelerate germination. Soak the seeds with the same volume of pure potato liquid medium that was not inoculated with Fusarium X-2-5 as a control.

[0035] Similarly, Hoagland’s nutrient solution containing cadmium, manganese, and zinc was configured, among which, two groups with different concentrations of cadmium were set at 10 μM and 50 μM, three groups with different concentrations of manganese were set at 100 μM, 500 μM, and 1000 μM, zinc Three groups with different concentrations were set up, namely 50 μM, 100 μM, and 5...

Embodiment 2

[0041] Configure a solid medium with grass bran and sawdust as the culture medium, ammonium nitrate as the exogenous nitrogen source, sucrose as the exogenous carbon source, and potassium dihydrogen phosphate as the exogenous phosphorus and potassium source, and adjust the water content to 65% and the pH to 6.5. Pack in polypropylene plastic bags, seal with a collar and film, and sterilize at 120°C for 90 minutes. After cooling, Fusarium X-2-5 is inoculated under aseptic conditions, and cultured at 25°C for 30-35 days in an indoor environment to prepare a solid microbial agent.

[0042] At the end of March or early April, select a 2m×2m plot in the non-polluted wasteland, electrolytic manganese waste dump and electrolytic zinc waste dump (both with cadmium pollution and lead pollution), dig the ground, loosen the soil, and apply deep Solid microbial agent, sprinkle water to keep moisture (about 70% water content), maintain soil balance, and cover with film to keep moisture for...

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Abstract

The invention discloses a heavy metal tolerant fusarium X-2-5 and application thereof. The preservation number of the fusarium X-2-5 is CGMCC No.11609, and the ITS1-5.8 S-ITS2 sequence of the fusarium X-2-5 is as shown in SEQ ID NO: 1; the fusarium X-2-5 and a bioremediation material containing the fusarium X-2-5 can be used for remediation of heavy metal contaminated soil, specifically, the fusarium X-2-5 or the bioremediation material is uniformly mixed with plant seeds and then sown, or the fusarium X-2-5 or the bioremediation material is directly placed in soil where plant seeds germinate or plants grow. The fusarium X-2-5 has strong heavy metal tolerance, can promote the growth of the plants on barren, water-deficient and heavy metal polluted land and improve the heavy metal enrichment capacity, is safe and environment-friendly, and has a good application prospect.

Description

technical field [0001] The invention relates to the technical field of environmental treatment, in particular to the discovery and characteristics of a heavy metal-tolerant Fusarium X-2-5, and the application of the bacteria in bioremediation of heavy metal-contaminated areas. Background technique [0002] With the rapid development of mining and metal smelting industries, heavy metal pollution in soil and groundwater in metal mining and smelting areas has become more and more serious, posing a huge threat to the production and life of surrounding residents, and is a key focus area for the prevention and control of heavy metal pollution. [0003] Heavy metals in mining areas migrate and diffuse mainly through leaching, metal-containing colloids, and organisms. The migration process is affected by many factors such as the chemical characteristics of heavy metals, soil organic matter content, and soil pH. Common pollutants in non-ferrous metal smelting sites mainly include cad...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14B09C1/10C12R1/77
CPCC12N1/14B09C1/105
Inventor 彭克俭罗岳平曾桂华钟振宇彭逸喆谭彬郭晟鹏
Owner 湖南省环境保护科学研究院
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