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Method for improving seedling hardening survival rate of tissue culture seedlings

A technology of tissue culture seedlings and survival rate, applied in the field of plant tissue culture, can solve problems such as death and wilting, and achieve the effects of enhancing metabolism, good economic and social benefits, and improving photosynthetic efficiency

Inactive Publication Date: 2021-11-30
SHANDONG SHOUGUANG VEGETABLE SEED IND GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Out of this environment, hardening and transplanting of seedlings is very easy to lose water and cause wilting, or death due to disease

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The healthy cherry tomato plant of tissue culture is used as the experimental material, and it is processed according to the following steps:

[0030] (1) Select the same batch of cherry tomato tissue culture seedlings with a plant height of 5 cm and a well-developed root system, place them in a culture bottle, and move the culture bottle to a greenhouse for 7 days of seedling training in a closed bottle. The shading degree should be 50%~ 70%, the temperature is not higher than 25 ℃. After hardening the seedlings in the closed bottle, use long tweezers to carefully remove 100 tissue culture seedlings with consistent growth from the agar medium, clean them thoroughly, and avoid damaging the root system. After that, dip the roots in 0.1% carbendazim solution and move them into the seedbed. The seedbed components are sterilized perlite, vermiculite and humus, and the mass ratio of perlite, vermiculite and humus is 1:1:0.5.

[0031] (2) After removing the tissue culture se...

Embodiment 2

[0049] Same as Example 1, the difference is that the healthy pepper plants of tissue culture are used as experimental materials.

Embodiment 3

[0067] Same as Example 1, the difference is that the healthy cucumber plants of tissue culture are used as experimental materials.

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PUM

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Abstract

The invention belongs to the technical field of plant tissue culture, and particularly relates to a method for improving the seedling hardening survival rate of tissue culture seedlings. According to the method, the tissue culture seedlings are transplanted into a seedling hardening matrix, and 0.1wt.% of algin is used for carrying out foliage spraying treatment on the tissue culture seedlings. Results of the embodiment show that the method provided by the invention can accelerate the strong growth of roots, stems and leaves of the tissue culture seedlings, so that the tissue culture seedlings can adapt to the external environment more quickly and better, the plant heights and the plant stems of the tissue culture seedlings are remarkably increased, and the survival rate of the tissue culture seedlings reaches 95% or above.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for improving the survival rate of tissue cultured seedlings. Background technique [0002] Tissue culture technology has great application potential for rescue preservation of important germplasm, rapid propagation of precious plant varieties, establishment of gene bank of germplasm resources, preservation, excavation and utilization of excellent germplasm resources. [0003] However, in the process of tissue culture, the tissue culture seedlings grow under the conditions of high humidity, low air permeability, weak light, constant temperature, and sufficient nutrients. Such growth environmental conditions form the unique morphological structure of the tissue culture seedling roots, stems, and leaves. and biological characteristics. Out of this environment, hardening and transplanting of seedlings is very easy to lose water and cause wilting, o...

Claims

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Application Information

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IPC IPC(8): A01G22/05A01C21/00
CPCA01G22/05A01C21/005
Inventor 程琳李金育武玉芬魏美甜韩宇睿郭建秋李晓杰王雪张娜杨浩东理查德·维萨
Owner SHANDONG SHOUGUANG VEGETABLE SEED IND GRP CO LTD