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Streptomyces neoformans and application thereof

A Streptomyces, YHG-1 technology, applied in the field of microorganisms, can solve difficult problems and achieve good inhibitory effects

Active Publication Date: 2021-11-30
HAIKOU EXPERIMENTAL STATION CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

More than a thousand species have been discovered in the genus Streptomyces, and it is difficult to rediscover a new species on this basis

Method used

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  • Streptomyces neoformans and application thereof
  • Streptomyces neoformans and application thereof
  • Streptomyces neoformans and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Separation and Identification of Sillet New YHG-1 Streptomyces

[0024] 1 experimental material

[0025] 1.1 Test soil

[0026] Collect the soil of the tropical rainforest rain forest in Hainan Province, which is placed in a sterile seal bag, sealing, and preserved in the ice box. After collecting, remove the root, stone, etc., preserved for the refrigerator 4 ° C.

[0027] 1.2 Test medium

[0028] The test medium of this experiment includes a medium separating medium, a culture characteristic observation, a physiological and biochemical medium and fermentation medium (Xu Lihua, 2007; Huang Xiaolong, 2009).

[0029] Table 1 Separation medium and formula

[0030]

[0031]

[0032] Table 2 Training characteristics observation medium and formulation

[0033]

[0034]

[0035] Table 3 Medicinal properties observation desired medium

[0036]

[0037] Table 4 Fermentation medium and its formula

[0038]

[0039]

[0040] 1.3 Reagents used in this experiment and equi...

Embodiment 2

[0116]Example 2 Antibacterial Activity Evaluation

[0117] 1 experimental method

[0118] Plate confrontation antibacterial activity evaluation

[0119] The labeling of strains was used to determine the strain of strains using a flat panel: the bacterial cake of purified seven-plant pathogens was taken by 5 mm punch, inoculated in the center of the PDA plate, respectively from the pathogenous bacteria 2.5cmA small amount of bacteria was inoculated at the four points at the place, with a petri dish in which only the pathogen was used as a blank control group, and each treatment was repeated 3 times.After 4 to 7 days of incubator in the 28 to 30 ° C incubator, the bacteriostatic belt width between the strain to be tested and the pathogen is measured and recorded.

[0120] 2 results

[0121] The bacteriostatic ratio of strain YHG-1 on rice blast is 69.4%.

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Abstract

The invention discloses streptomyces neoformans YHG-1, and the preservation number of the streptomyces neoformans YHG-1 is CCTCC (China Center for Type Culture Collection) NO: M2021007. The streptomyces neoformans YHG-1 has a growth pH range of 5-8 and an optimal growth pH of 7, can normally grow on a culture medium with NaCl content of not more than 7%, can generate various enzymes, has antibacterial activity, and has a good application prospect in the fields of enzyme industry and agriculture.

Description

Technical field [0001] The present invention belongs to the microbial technology, and therefore refers to a new streptoma and its application. Background technique [0002] Streptomyces is a Gram-positive bacteria with filamentous branched mycella, with complex morphological differentiation cycles and strong secondary metabolic capabilities, which can produce many biologically active metabolism, which is a huge economy and practical. Value microbial resources, it is important in the fields of agriculture, food and medicine. There are more than a thousand kinds of streptoma, some streptomae can produce a variety of antibiotics, and some kinds of vitamins, organic acids, alkaloids, enzymes, and the like. [0003] Since there are physiological properties and functional differences between different species in streptoma, streptavia is widely used in various fields. There are already more than a thousand kinds of seeds found in streptoma. On this basis, a new species is more difficult...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N9/16C12N9/28C12N9/52A01N63/28A01P3/00C12R1/465
CPCC12N1/20C12N9/16C12N9/2417C12N9/52A01N63/28Y02A40/10
Inventor 井涛臧小平谢江辉周登博云天艳王尉陈宇丰
Owner HAIKOU EXPERIMENTAL STATION CHINESE ACAD OF TROPICAL AGRI SCI