Micro-fluidic chip, system and method for detecting biochemical elements of water body

Abstract

The invention discloses a micro-fluidic chip, a system and a method for detecting biochemical elements of a water body. The micro-fluidic chip comprises a chip body, and a chip flow path is arranged in the chip body; the chip flow path comprises a sample injector connecting channel, a multipurpose connecting channel, a liquid storage pipeline, a water sample and reagent cross mixing area, a reagent connecting channel, a reaction pipeline and an internal optical path detection pool. The micro-fluidic chip for detecting the biochemical elements of the water body, provided by the invention, can be externally connected with liquid core waveguide pipelines with different lengths according to requirements besides an internal optical path detection pool, so that long-optical-path high-sensitivity detection can be realized, and high-precision detection of trace biochemical elements is realized. Compared with a traditional micro-fluidic detection system using a plurality of sample injectors or injection pumps, the detection system provided by the invention only needs one sample injector to realize cleaning of a flow path and sample injection and reaction of each sample and reagent, so that the volume and power consumption of a sample injection device of the system are greatly reduced.

Description

technical field [0001] The invention relates to the field of microfluidic chip laboratories, in particular to a microfluidic chip, system and method for detecting water body biochemical elements. Background technique [0002] Since the development of microfluidic chips in the 1990s, a large number of applications have been realized in the fields of chemistry, biology, and medicine. In the field of detection of water biochemical elements, the existing microfluidic chips have the following deficiencies: [0003] 1. The detection sensitivity is limited. Most of the existing microfluidic chips are made of PMMA, glass, PEI, etc. The material does not have total reflection characteristics. Limited by the material of the microfluidic chip, the length of the colorimetric cell does not exceed 10cm, resulting in low detection sensitivity and the inability to achieve trace element detection. And once the flow path of the microfluidic chip is determined, the length of the colorimetri...

AI Technical Summary

Problems solved by technology

Most of the existing microfluidic chips are made of PMMA, glass, PEI, etc. The material does not have total reflection characteristics. Limited by the material of the microfluidic chip, the length of the colorimetric cell does not exceed 10cm, resulting in low detection sensitivity and the inability to achieve trace detection of elements

And once the flow path of the microfluidic chip is determined, the length of the colorimetric cell is fixed. If it is necessary to adjust the optical path length of the colorimetric cell to adapt to different ranges of measurement, the microfluidic chip can only be redesigned and processed, and the processing and time costs are high. Poor flexibility of use;

[0004] 2. The flow path of the existing microfluidic chip adopts unidirectional flow injection analysis or "stop flow" analysis technology, that is, the reaction of water samples and reagents in the flow path of the microfluidic chip needs to increase the reaction time or prolong the reaction flow path To achieve, reducing the system detection timeliness and integration;

[0005] 3. The sampling device adopts multiple samplers or syringe pumps, and the power consumption and volume of the system are relatively large

[0006] Patent document CN105562131 A discloses a microfluidic chip, detection system and detection method for total phosphorus detection. Although the chip can realize online monitoring, the length of the detection pool is fixed and the structure is relatively complicated.

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