Method for screening salt-tolerant or salt-sensitive rice seeds in germination period
A technology for rice seeds and screening methods, which is applied in germination equipment, rice cultivation, data processing applications, etc., can solve problems such as the exploration of salt tolerance identification has not been carried out, it is difficult to fully and accurately reflect plant salt tolerance, and photosynthesis is affected.
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Embodiment 1
[0033] Example 1 Growth Index of Rice Varieties Germinated under Different Salt Concentrations
[0034] (1) The rice seeds were placed on three layers of germination paper moistened with 0, 150 or 200 mM saline for germination test, 4 repetitions, 100 seeds per repetition. The seeds were placed in a 30°C (16 hours dark) / 20°C (8 hours light) incubator with a light intensity of 250 μmol m -2 ·s -1 . Taking the seed radicle breaking through the seed coat as the germination standard, the number of germinations was recorded every day. The germination potential was counted on the 5th day of germination, and the germination rate and seedling rate were counted on the 14th day. When counting the germination rate, 40 seedlings were randomly selected to measure the fresh weight, and then the samples were placed in an oven at 80°C for 24 hours, taken out, cooled in a desiccator, and then measured for their dry weight. Finally, the germination index, vigor index and average germination...
Embodiment 2
[0057] Example 2 Salt Tolerance Screening of Indica and Japonica Rice Varieties
[0058] The specific screening method is as follows:
[0059] (1) Take 18 kinds of rice seeds to be screened (namely: Qianyou 1, Lingyou Huazhan, Nei 5 You 8015, Jing Liang You 534, Zhu Liang You 06, Y Liang You 689, Meng Liang You Hua Zhan, Zhejiang Liangyou 274, Jiazaofeng 18, Yongxian 15, Chunyou 84, Chunyou 927, Jiafengyou 2, Jiayou 5, Yongyou 10, Chunjiangnuo 6, Xiushui 121 and Jia 58), The rice seeds were respectively placed on three layers of germination paper moistened with 0 (clear water), 150 or 200 mM saline to carry out the germination test, 4 repetitions, 100 seeds per repetition. The seeds were placed in a 30°C (16 hours dark) / 20°C (8 hours light) incubator with a light intensity of 250 μmol m -2 ·s -1 . Then calculate the difference between each growth index value of rice seeds under different salt concentrations and each growth index value under zero salt concentration, which i...
Embodiment 3
[0090] Example 3 Salt Tolerance Screening of Hybrid Rice and Conventional Rice Varieties
[0091] The specific screening method is as follows:
[0092] (1) Take 18 kinds of rice seeds to be screened (namely: Qianyou 1, Lingyou Huazhan, Nei 5 You 8015, Jing Liang You 534, Zhu Liang You 06, Y Liang You 689, Meng Liang You Hua Zhan, Zhejiang Liangyou 274, Jiazaofeng 18, Yongxian 15, Chunyou 84, Chunyou 927, Jiafengyou 2, Jiayou 5, Yongyou 10, Chunjiangnuo 6, Xiushui 121 and Jia 58), The rice seeds were respectively placed on three layers of germination paper moistened with 0 (clear water), 150 or 200 mM saline to carry out the germination test, repeated 4 times, 100 seeds per repetition. The seeds were placed in a 30°C (16 hours dark) / 20°C (8 hours light) incubator with a light intensity of 250 μmol m -2 ·s -1. Then calculate the difference between each growth index value of rice seeds under different salt concentrations and each growth index value under zero salt concentrati...
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