Application of corn Zm00001d029151 gene in regulation and control of morphogenesis of guard cells

A technology of guard cells and morphogenesis, applied in the field of genetic engineering, can solve the problems such as the unclear regulation mechanism of dumbbell-shaped guard cells, and achieve the effect of improving water use efficiency

Active Publication Date: 2021-12-07
SANYA INST OF HENAN UNIV +1
View PDF5 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, considerable progress has been made in the study of the stomatal complex in grasses,

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of corn Zm00001d029151 gene in regulation and control of morphogenesis of guard cells
  • Application of corn Zm00001d029151 gene in regulation and control of morphogenesis of guard cells
  • Application of corn Zm00001d029151 gene in regulation and control of morphogenesis of guard cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] biomaterials:

[0048] After Zm00001d029151 mutations (transposon insertion, deletion or knockout bases) inactivation, i.e. Zm00001d029151 maize gene can not be expressed, a mutant (i.e. Zm00001d029151 maize mutant gene, designated herein as bzu3: bizui3) plants guard cells not normal dumbbell shape but a linear or rod-like.

[0049] Bzu3-1 mutants and the corresponding wild type, is publicly available material from Maize GeneticsCooperation Stock Center.

[0050] Bzu3-2 a mutant derived from EMS (Ethyl methylsulfone) Mutagenesis Mutants of Maize Inbred Zheng58 created.

[0051]The empty support used by corn transgenes PBUE411, PCM3300M-GFP and PCAMBIA3301 are provided by China Agricultural University. The laboratory was built into PBUE411: CAS9-BZU3; PCM3300M-GFP: BZU3PRO :: BZU3-Venus; PCambia3301: BZU3PRO :: GUS.

[0052] It is to be explained that the BZU3 mutant gene is not confirmed prior to the present application, that is, which gene in the BZu3 mutant is unknown is...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to the technical field of gene engineering, and particularly discloses application of a corn Zm00001d029151 gene in regulation and control of morphogenesis of guard cells, the nucleotide sequence of the Zm00001d029151 gene is shown as SEQ ID NO.1, the amino acid sequence of protein coded by the Zm00001d029151 gene is shown as SEQ ID NO.2, and the Zm00001d029151 gene is specifically expressed in the guard cells and participates in regulation and control of the thickening process of cell walls of the guard cells. The Zm00001d029151 gene is used for regulating and controlling morphogenesis of dumbbell-shaped guard cells, and has important theoretical significance and practical application value for exploring a molecular mechanism of morphogenesis of corn stomatal guard cells and improving stress resistance of corn.

Description

Technical field [0001] The present invention relates to the field of genetic engineering, particularly to the use of the gene in maize Zm00001d029151 built aspect of the regulation of guard cell morphology. Background technique [0002] Cell wall is one of the important structural features of plant cells from the animal cells, cell wall composition and structure determines the morphology and function of cells, plays an important role in plant development and in response to the environment. And is one of surface pores highly specialized organ, plant gas exchange with the outside important "gateway", is different from other epidermal cells, guard cells having a non-uniform cell wall thickening and repeated stretching, opening and closing the pores make it adapt to changes in the process volume. Dumbbell shaped pores grasses may be one of the most sensitive reactions hole type, compared with other types of pores can be more quickly opened and closed, and improved water use efficienc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N15/61C12N9/90C12N15/82A01H5/00A01H6/46
CPCC12N9/90C12N15/8261C12Y501/03002
Inventor 宋纯鹏周玉森郭思义李保珠乔鑫
Owner SANYA INST OF HENAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap