Method for rapidly distinguishing mint aqueous extract from spearmint aqueous extract

A technology of peppermint water and water extract, applied in the directions of measuring devices, instruments, scientific instruments, etc., can solve the problem of inability to accurately distinguish spearmint perfume extract and peppermint water extract, etc., and achieve rapid identification, accurate identification, and guarantee. The effect of the test results

Active Publication Date: 2021-12-07
BEIJING KANGRENTANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Therefore, the technical problem to be solved in the present invention is to overcome the problem that the prior art cannot accurately distinguish the spearmint water extract and the spearmint water extract; thereby providing a method for quickly distinguishing the peppermint water extract and the spearmint water extract

Method used

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  • Method for rapidly distinguishing mint aqueous extract from spearmint aqueous extract
  • Method for rapidly distinguishing mint aqueous extract from spearmint aqueous extract
  • Method for rapidly distinguishing mint aqueous extract from spearmint aqueous extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] A method for quickly distinguishing the water extract of peppermint and the extract of spearmint water, the specific process is as follows:

[0043] 1. Preparation of water extract:

[0044] The decoction pieces of mint and spearmint are prepared into water extract. in,

[0045] Preparation of mint water extract: take mint decoction pieces, soak for 30 minutes, decoct twice, add 15 times the amount of water to the first decoction, decoct for 20 minutes, add 12 times the amount of water to the second decoction, decoct for 15 minutes, and the liquid Heat and filter with a 150-mesh filter cloth, combine the two filtrates, and dry to obtain the peppermint water extract.

[0046] Preparation of spearmint water extract: take spearmint decoction pieces, soak for 30 minutes, decoct twice, add 15 times the amount of water to the decoction in the first decoction, decoct for 20 minutes, add 12 times the amount of water to decoction in the second decoction, decoct for 15 minutes,...

Embodiment 2

[0062] A method for quickly distinguishing mint aqueous extract and spearmint water extract, the difference from Example 1 is that the gradient elution procedure is different in high performance liquid chromatography, and the gradient elution procedure is shown in the following table 2 in the present embodiment Show.

[0063] Table 2

[0064]

[0065] Other conditions in the present embodiment are all identical with embodiment 1, and the chromatogram that detects obtains is as follows figure 2 As shown, among them, the resolution of the characteristic peaks corresponding to mongoside and rosmarinic acid are all ≥1.2.

Embodiment 3

[0067] In this example, the same method as in Example 1 is adopted to verify the collected 10 batches of peppermint and spearmint. The batch numbers and origins of the 10 batches of mint and spearmint are shown in Table 3.

[0068] table 3

[0069]

[0070]

[0071] The above-mentioned mint and spearmint from different origins are prepared into water extracts by the method described in Example 1, and the water extracts are only marked with batch numbers. Detected, obtained 10 batches of water extracts of montanoside and rosmarinic acid corresponding to the peak area of ​​the characteristic peak, and with the chromatographic peak of rosmarinic acid as the reference peak S, to obtain the relative peak area of ​​each characteristic peak , as shown in Table 4.

[0072] Simultaneously, record the batch number of the characteristic peak that three peak heights continue to decrease in the characteristic peak section that the relative retention time is 0.82 ± 15% in the record...

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Abstract

The invention provides a method for rapidly distinguishing a mint aqueous extract from a spearmint aqueous extract. The method comprises the following steps: acquiring a characteristic chromatogram of an object to be detected by adopting a high performance liquid chromatography, wherein the characteristic chromatogram at least comprises characteristic peaks corresponding to the linarin and the rosmarinic acid, taking a chromatographic peak of the rosmarinic acid as a reference peak S, and calculating the relative peak area of the characteristic peak corresponding to the linarin and the reference peak S; when the relative peak area is greater than or equal to 1, determining that the aqueous extract is the spearmint aqueous extract; when the relative peak area is less than 1, determining that the aqueous extract is the mint aqueous extract; when three continuous characteristic peaks with continuously reduced peak heights appear in a characteristic peak section with the relative retention time of 0.82 + / -15% in the characteristic chromatogram, determining that the aqueous extract corresponding to the characteristic chromatogram is the spearmint aqueous extract; and when three continuous characteristic peaks of which the peak heights are continuously reduced do not appear in the corresponding characteristic peak section, determining that the aqueous extract corresponding to the characteristic spectrum is the mint aqueous extract. According to the method, the mint aqueous extract and the spearmint aqueous extract can be quickly and accurately distinguished, and the operation is very simple and convenient.

Description

technical field [0001] The invention relates to the field of identification of traditional Chinese medicines, in particular to a method for rapidly distinguishing mint water extract and spearmint water extract. Background technique [0002] Mint is the dry aerial part of Mentha haplocalyxB riq, and spearmint is the dry aerial part of Menthaspicata L., a plant of Lamiaceae. Peppermint is a special Chinese medicinal material in my country, and it is also exported in large quantities as a raw material for the spice industry. Spearmint is native to Europe and is widely cultivated all over the world. It is cultivated in Jiangsu, Hebei, Zhejiang, Sichuan and other places in my country. Its volatile oil is widely used in fructose, toothpaste and other products, and its function is as a spice. [0003] Spearmint is a common counterfeit of mint in recent years. Although the two belong to the same labiatae plant, their smell and chemical composition are different, so they should not ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/86
CPCG01N30/02G01N30/06G01N30/8679G01N2030/065Y02A50/30
Inventor 张志强史国华贾扬花付静
Owner BEIJING KANGRENTANG PHARMA
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