Five SNPs markers related to grass carp fructose-1, 6-diphosphatase and application
A marker, grass carp technology, applied in recombinant DNA technology, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problems of slow growth of offspring, poor disease resistance, poor quality of parents, etc., to achieve a wide range of applications, The effect of quick detection
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Embodiment 1
[0040] 1 Materials and methods
[0041] 1.1 Experimental fish
[0042] SNPs screening samples: Grass carp samples for screening the SNPs of fructose-1,6-bisphosphatase gene of grass carp were collected from Ningxiang, Hunan Province, Yuanjiang, Hubei Province, Jianli, Hubei Province, Shishou, Hubei Province, Honghu and Guangdong Province, respectively. There were 4 grass carp breeding groups in Nanzhuang, with a total of 24 samples, and the tail fins were clipped for genomic DNA extraction.
[0043]Growth trait association analysis samples: Grass carp samples for growth trait association analysis were taken from Baijin Seedling Co., Ltd., Foshan City, Guangdong Province. In the same batch of 17-month-old grass carp reared in the same batch, 296 grass carp samples were randomly selected for trait association analysis. The growth data of body weight, body length, body height and body width of the samples were measured, and the caudal fins were cut and stored in absolute ethano...
Embodiment 2
[0071] Popgene 32 (version 3.2) software calculates genetic parameters such as effective allele number (Ne), observed heterozygosity (Ho), expected heterozygosity (He) and allele frequency of SNPs loci. Using the general linear model (GLM) of SPSS 17.0 software to calculate the genotypes and growth traits (body weight, body length, body height, body width) and The correlation between the plumpness and the least-significant difference (LSD) analysis were performed. The plumpness formula is: K=(W / L3)×100%, where K is the plumpness and W is the fish Body weight (g), L is the fish body length (cm). The results are shown in Table 6.
[0072] Table 6 Association analysis between different genotypes and growth traits of 5 SNPs
[0073]
[0074] Note: Different lowercase letters indicate significant differences (P<0.05), and different capital letters indicate extremely significant differences (P<0.01).
[0075] According to spss results, the genotypes of SNP1, SNP2, SNP3, SNP4, ...
Embodiment 3
[0077] This example studies the association analysis of grass carp fructose-1,6-bisphosphatase gene SNPs diplotypes and growth traits.
[0078] The association analysis of fructose-1,6-bisphosphatase gene SNPs and growth traits of grass carp was carried out by using population genetics research method (linkage disequilibrium (LD) analysis).
[0079] LD analysis using SHEsis, where r 2 for metrics.
[0080] r 2 The larger the value, the larger the degree of LD. when r 2 >0.33, indicating the presence of strong LD.
[0081] The result is as Image 6 ( Image 6 Left is the D' value, Image 6 right is r 2 value).
[0082] The linkage disequilibrium analysis of five SNPs in the fructose-1,6-bisphosphatase gene of grass carp was performed using SHEsis online software ( Image 6 , Image 6 Left is the D' value, Image 6 right is r 2 value), it was found that SNP1 was not linked with SNP2 and SNP4 (r 2 2 2 ≈0), there is linkage disequilibrium between SNP2 and SNP4 (r 2 ...
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