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Five SNPs markers related to grass carp fructose-1, 6-diphosphatase and application

A marker, grass carp technology, applied in recombinant DNA technology, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problems of slow growth of offspring, poor disease resistance, poor quality of parents, etc., to achieve a wide range of applications, The effect of quick detection

Pending Publication Date: 2021-12-10
PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Grass carp in natural water bodies is slender, low in fat, and grows slowly. Artificially raised grass carp is mainly fed with compound feed. Although it grows fast, most of the belly fat accumulates, which is not liked by consumers.
So far, although aquatic workers have made a lot of efforts, there are still no artificially selected fine varieties of grass carp that have passed the identification of the National Fine Variety Approval Committee. In actual production, most of them are directly domesticated from wild species, lacking directional selection, and production. The poor quality of the parents often causes problems such as slow growth, uneven specifications, and poor disease resistance of the offspring, and farmers often face major economic losses. Therefore, breeding high-yield and high-quality grass carp varieties has always been the central goal of grass carp breeding.

Method used

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  • Five SNPs markers related to grass carp fructose-1, 6-diphosphatase and application
  • Five SNPs markers related to grass carp fructose-1, 6-diphosphatase and application
  • Five SNPs markers related to grass carp fructose-1, 6-diphosphatase and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] 1 Materials and methods

[0041] 1.1 Experimental fish

[0042] SNPs screening samples: Grass carp samples for screening the SNPs of fructose-1,6-bisphosphatase gene of grass carp were collected from Ningxiang, Hunan Province, Yuanjiang, Hubei Province, Jianli, Hubei Province, Shishou, Hubei Province, Honghu and Guangdong Province, respectively. There were 4 grass carp breeding groups in Nanzhuang, with a total of 24 samples, and the tail fins were clipped for genomic DNA extraction.

[0043]Growth trait association analysis samples: Grass carp samples for growth trait association analysis were taken from Baijin Seedling Co., Ltd., Foshan City, Guangdong Province. In the same batch of 17-month-old grass carp reared in the same batch, 296 grass carp samples were randomly selected for trait association analysis. The growth data of body weight, body length, body height and body width of the samples were measured, and the caudal fins were cut and stored in absolute ethano...

Embodiment 2

[0071] Popgene 32 (version 3.2) software calculates genetic parameters such as effective allele number (Ne), observed heterozygosity (Ho), expected heterozygosity (He) and allele frequency of SNPs loci. Using the general linear model (GLM) of SPSS 17.0 software to calculate the genotypes and growth traits (body weight, body length, body height, body width) and The correlation between the plumpness and the least-significant difference (LSD) analysis were performed. The plumpness formula is: K=(W / L3)×100%, where K is the plumpness and W is the fish Body weight (g), L is the fish body length (cm). The results are shown in Table 6.

[0072] Table 6 Association analysis between different genotypes and growth traits of 5 SNPs

[0073]

[0074] Note: Different lowercase letters indicate significant differences (P<0.05), and different capital letters indicate extremely significant differences (P<0.01).

[0075] According to spss results, the genotypes of SNP1, SNP2, SNP3, SNP4, ...

Embodiment 3

[0077] This example studies the association analysis of grass carp fructose-1,6-bisphosphatase gene SNPs diplotypes and growth traits.

[0078] The association analysis of fructose-1,6-bisphosphatase gene SNPs and growth traits of grass carp was carried out by using population genetics research method (linkage disequilibrium (LD) analysis).

[0079] LD analysis using SHEsis, where r 2 for metrics.

[0080] r 2 The larger the value, the larger the degree of LD. when r 2 >0.33, indicating the presence of strong LD.

[0081] The result is as Image 6 ( Image 6 Left is the D' value, Image 6 right is r 2 value).

[0082] The linkage disequilibrium analysis of five SNPs in the fructose-1,6-bisphosphatase gene of grass carp was performed using SHEsis online software ( Image 6 , Image 6 Left is the D' value, Image 6 right is r 2 value), it was found that SNP1 was not linked with SNP2 and SNP4 (r 2 2 2 ≈0), there is linkage disequilibrium between SNP2 and SNP4 (r 2 ...

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Abstract

The invention discloses five SNPs markers related to grass carp fructose-1, 6-diphosphatase and application of the five SNPs markers. The SNPs markers are respectively as shown in at least one of SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4 and SEQ ID NO. 5. The invention finds that the individual growth traits of which the marker SEQ ID NO.1 is CC genotype and / or the marker SEQ ID NO.3 is AA genotype and / or the marker SEQ ID NO.5 is AC genotype and / or the marker SEQ ID NO.1 is CT genotype, the marker SEQ ID NO.3 is AA genotype and the marker SEQ ID NO.5 is AA genotype have significant advantages, and the SNPs marker can be used for simply and quickly identifying the grass carp with fast growth and slender body type, thereby providing a basis for the molecular marker-assisted selective breeding of the grass carp.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and specifically relates to five SNPs markers and applications related to fructose-1,6-bisphosphatase of grass carp. Background technique [0002] Grass carp (Ctenopharyngodonidellus) belongs to the Cyprinidae (Leuciscinae) and the Ctenopharyngodon subfamily (Ctenopharyngodon), which has a long history of breeding in my country. In 2018, my country's grass carp aquaculture production reached 5.5 million tons, accounting for 21% of freshwater aquaculture fish production. In my country, grass carp farming is very extensive, and there are a total of 30 provinces (municipalities and autonomous regions) farming. In 2018, Guangdong Province The highest yield of grass carp. Grass carp in natural water is slender, with low fat content and slow growth. Artificially farmed grass carp is mainly fed with compound feed. Although the growth rate is fast, most of the abdominal fat accumulates, which i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6869C12N15/11
CPCC12Q1/6888C12Q1/6869C12Q1/42C12Q2600/124C12Q2600/156C12Q2600/16C12Q2531/113C12Q2537/143
Inventor 陈在佳樊佳佳朱华平马冬梅钟再选苏换换
Owner PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI