Method for testing adulteration of radix ophiopogonis in infantile Yanbian granules

Abstract

An ultra-high performance liquid chromatography and mass spectrometry combined method is adopted to detect whether the infantile Yanbian granules contain radix ophiopogonis adulterated components liriope spicata saponin B and liriope muscari saponin C or not. The method specifically comprises the following steps: respectively preparing a test solution and a reference solution with certain concentrations, respectively detecting by adopting an ultra-high performance liquid chromatography-mass spectrometer, respectively obtaining selected ion chromatograms of the test solution and the reference solution, and then carrying out adulteration analysis; and in the selected ion chromatograms of the test solution and the reference solution, when the peak appearance time is the same and the peak area ratio is consistent, regarding as adulteration. According to the detection method disclosed by the invention, the interference of other seven medicines in the infantile Yanbian granules can be eliminated; meanwhile, the method has the characteristics of strong specificity, relatively good sensitivity, good precision, good repeatability, strong stability and high recovery rate; and the method can detect liriope spicata which is adulterated by 10% or more on the market.

Description

technical field [0001] The invention relates to a method for testing the adulteration of Ophiopogon japonicus, in particular to a method for testing the adulteration of Ophiopogon japonicus in Xiaoeryanbian granules. Background technique [0002] Xiaoeryanbian granule is a class A non-prescription drug. The prescription is composed of honeysuckle, shegan, golden fruit olive, bellflower, scrophulariaceae, Radix Ophiopogon japonicus, artificial bezoar (bezoar cultivated in vitro) and borneol. The prescription amount of Ophiopogon japonicus is 78.1g. The proportion of prescriptions in preparations is the third. In the prescription, honeysuckle clears heat and detoxifies, clears away phlegm and evacuates, shoots dried phlegm and sore throat, and is the king drug; Jin Guolan clears throat and relieves pain, platycodon grandiflorum opens lung qi, reduces phlegm and relieves throat, Scrophulariaceae and Ophiopogon japonicus nourishes yin, moistens dryness, and loosens phlegm. Cond...

AI Technical Summary

Problems solved by technology

There are often some defects in the current detection method. (1) When the TLC method is used to check the characteristic components of Lime japonicus, the sensitivity is insufficient, the specificity is not strong, and the interference of spots leads to false positives. Whether there is any adulteration of Limedong in the flat granule; (2) When the high performance liquid chromatography evaporative light (HPLC-ELSD) method is used to check the characteristic components of Lipe, the sensitivity is low and the specificity is poor, and Lipe cannot be detected Characteristic components, leading to false negative results

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