Unlock instant, AI-driven research and patent intelligence for your innovation.

Cryogenic freezing method of scaffolds for tissue repair

A low-temperature freezing, tissue repair technology, used in tissue culture, bone/connective tissue cells, biochemical equipment and methods, etc.

Active Publication Date: 2022-07-29
章毅 +7
View PDF10 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When preparing engineered tissues for clinical applications, cryopreservation strategies have the potential to enable off-the-shelf tissue-engineered constructs

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Cryogenic freezing method of scaffolds for tissue repair
  • Cryogenic freezing method of scaffolds for tissue repair
  • Cryogenic freezing method of scaffolds for tissue repair

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0033] The preparation method of the gallic acid grafted chitosan scaffold of the present invention is as follows:

[0034] 28 mmol of gallic acid and 2.8 mmol of EDC were first dissolved in 40 mL of 70% ethanol and 2.8 mmol of NHS was added to the solution. The resulting solution was stirred in an ice bath, then 1.5 g of chitosan dispersed in 110 mL of 70% ethanol was added after 1 hour. The solution was further stirred in an ice bath for 30 minutes and finally at room temperature for 24 hours. The precipitate was collected by filtration and washed with ethanol. After dialysis against deionized water for 3 days to remove possible residual reagents, gallic acid-grafted chitosan was obtained by vacuum freeze-drying. Porous gallic acid-grafted chitosan scaffolds were prepared by utilizing the physical properties of gallic acid-grafted chitosan being dissolved in an acidic solution and insoluble in an alkaline condition. First, 1 g of gallic acid grafted chitosan was dissolved...

Embodiment 1

[0044] For the survival rate of mesenchymal stem cells in tissue engineering structures, see figure 1 shown.

[0045] The proliferative ability of mesenchymal stem cells in tissue engineered structures see image 3 shown.

[0046] Colony-forming ability of mesenchymal stem cells in tissue engineered structures see Figure 4 shown, the three differentiation capabilities of mesenchymal stem cells in tissue-engineered structures are shown in Figure 5 shown.

Embodiment 2

[0048] The tissue engineered structures were cryopreserved in 10% DMSO-containing fetal bovine serum (90% FBS) cryopreservation tubes. The cryovials were then sequentially stored at 4°C for 30 minutes, -20°C for 60 minutes, -80°C overnight, and finally stored under liquid nitrogen for 30 days.

[0049] For the survival rate of mesenchymal stem cells in tissue engineering structures after cryopreservation, see figure 1 shown. For the survival rate of mesenchymal stem cells in tissue engineering structures after cryopreservation, see figure 2 shown.

[0050] The proliferation ability of mesenchymal stem cells in tissue engineered structures after cryopreservation is shown in image 3 shown, the colony-forming ability of mesenchymal stem cells see Figure 4 shown, the three differentiation capabilities of mesenchymal stem cells are shown in Figure 5 shown.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A low-temperature freezing method for a scaffold for tissue repair, the scaffold for tissue repair and mesenchymal stem cells are cultured in a 24-well culture plate; the medium is changed every 2 days, and after 7 days of culture, a well-structured tissue engineering structure is formed; The prepared tissue engineering structures were immersed in cryoprotectant and incubated in an incubator for 12 hours; the scaffolds for tissue repair were made of gallic acid-grafted chitosan. The cryogenic freezing method of the present invention is suitable for cryogenic cryopreservation of tissue engineering structures (such as scaffolds) containing living cells, maintains the cell survival rate and recovery rate at a relatively high level, and enables frozen stem cells to continue after recovery Maintain dryness and differentiation potential.

Description

technical field [0001] The present invention relates to a method for preserving biological products, in particular to a method for cryogenically freezing a scaffold for tissue repair, so as to maintain the biological activity of biological products (eg, cells) in the scaffold. Background technique [0002] Tissue engineering and regenerative medicine are considered a promising approach to repair damaged tissues and maintain biological functions. Among the therapeutic approaches in tissue engineering and regenerative medicine, cell-based therapy is one of the recent hotspots. These therapies have been developed to regenerate damaged tissue and provide many beneficial cytokines. In particular, the implantation of stem cells in the scaffold allowed the stem cells to maintain their stemness. In previous studies, we established tissue engineering constructs based on gallic acid-grafted chitosan scaffolds and mesenchymal stem cells. In addition, the difficulty of scaffold fabri...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02C12N5/0775C08B37/00
CPCA01N1/0221A01N1/0226C12N5/0662C08B37/006C12N2533/72
Inventor 章毅蔡海波王进伍婷陈亮
Owner 章毅