HPLC-MS/MS method for simultaneously determining concentrations of 14 antidepressants in human plasma

Abstract

The invention discloses an HPLC-MS / MS (High Performance Liquid Chromatography-Mass Spectrometry / Mass Spectrometry) method for simultaneously determining the concentrations of 14 antidepressants in human plasma, analytes in a plasma sample are extracted by adopting a protein precipitation method, the sample is separated by adopting an Agilent Infinity Lab Poroshell 120EC-C18 chromatographic column, acetonitrile and water are used as mobile phases for gradient elution, and the mass spectrum condition adopts an electrospray ion source positive ion mode and a multi-ion monitoring mode. The total time of chromatographic separation is 4 minutes. According to the present invention, the 14 antidepressants detected by the method have good linearity (R2 > 0.99) in the blank plasma, the intra-batch and inter-batch precision RSD is 0.65-13.18%, the accuracy is 85.21-117.6%, the matrix effect, the recovery rate, the stability, the dilution effect, the residual effect and the like meet the requirements, and the significant clinical data is provided for the optimization of the clinical drug treatment scheme.

Description

technical field [0001] The invention relates to the technical field of clinical blood drug concentration monitoring, in particular to an HPLC-MS / MS method for simultaneously measuring the concentrations of 14 kinds of antidepressants in human plasma. Background technique [0002] In recent years, with the development of mental health, the prevention and treatment of mental illness has received more and more attention from all walks of life. However, the treatment of mental illness is extremely challenging, which is mainly reflected in the poor medication compliance of psychiatric patients, narrow drug treatment window, and serious side effects. Therapeutic drug monitoring (TDM) plays a crucial role in the treatment of psychiatric disorders. There has been a large body of evidence that monitoring serum or plasma drug concentrations has therapeutic and economic benefits, not only providing patient compliance, possible therapeutic response, and drug Kinetic-related data, but a...

AI Technical Summary

Problems solved by technology

Therefore, it is still a great challenge to efficiently measure multiple compounds simultaneously

In addition, although a large number of analytical methods reported in the literature used liquid-liquid extraction (LLE) or solid-phase extraction (SPE) as a sample pretreatment method, these analytical methods are time-consuming and expensive reagent consumables

Some efficient and environmentally friendly sample preparation methods have been developed in recent reports, such as liquid phase microextraction (LPME), solid phase microextraction (SPME) and tandem dispersion liquid-liquid microextraction (TDLLME) methods, which provide better Purification ability, but complex and expensive, not suitable for routine clinical testing

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