Hollow fiber tube for human cancer tissue transplantation tumor and application of hollow fiber tube
A fibrous tube and cancer tissue technology, applied in the medical field, can solve the problem of lack of screening and verification platform for tumor molecular markers, and achieve the effect of reducing the dosage and shortening the experimental period.
Pending Publication Date: 2021-12-24
东部细胞治疗(山东)有限公司
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Problems solved by technology
At present, the lack of rapid and effective screening and verification platform is the bottleneck of tumor molecular marker research
Method used
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[0023] For ease of understanding, the technical solutions of the present invention are further specifically described below in conjunction with the accompanying drawings and through embodiments:
[0024] 1. Experimental materials
[0025] 1.1. Experimental animals
[0026] Nude mice of Balb / CSPF grade, female, 6-8 weeks old, were purchased from Shanghai Slack Experimental Animal Co., Ltd., production license number: SCXK (Shanghai) 2007-(8)05.
[0027] 1.2. Reagents
[0028] RPM-1640 medium: Gino Biomedical Technology Co., Ltd.;
[0029] Fetal bovine serum: Gibco, USA.
[0030] 1.3. Instruments
[0031] 37°CCO 2 Constant temperature incubator: BINDER, Germany;
[0032] Heal Force ultra-clean workbench: Likang Biomedical Technology Holdings Co., Ltd.;
[0033] Inverted phase contrast microscope: ZEISS, Germany;
[0034] -80℃ ultra-low temperature refrigerator: Haier Company;
[0035] PVDF tube with a molecular cut-off of 58kDa and an inner diameter of 1.0mm (Spectrum M...
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Abstract
The invention relates to a hollow fiber tube for a human cancer tissue transplantation tumor and an application of the hollow fiber tube. The hollow fiber tube is cut into appropriate lengths and soaked in 70% alcohol to be placed at the room temperature for 72 h or above; the inner wall of the hollow fiber tube is washed with deionized water for three times one by one, and the hollow fiber tube is soaked in the deionized water for more than 24 hours; sterilization at high temperature and high pressure is achieved, and refrigerating and storing are achieved at 4 DEG C for later use. According to the hollow fiber tube for the human cancer tissue transplantation tumor and the application of the hollow fiber tube, by combining the hollow fiber tube with a tissue sample of a cancer patient and applying the hollow fiber tube to establishment of a mouse model, a set of relatively complete animal model experiment method can be constructed to test an individualized medication strategy for the cancer patient, and human-mouse combined clinical test is realized in the future so as to be used for accurate treatment research.
Description
[0001] This application is the application number 201911027732.9 (name: a method for establishing a mouse model of human cancer tissue transplanted tumor hollow fiber test method; application date: October 28, 2019; priority number: CN201910914854.3; priority date: The divisional application of the invention application on September 26, 2019). technical field [0002] The invention relates to the field of medicine, in particular to a hollow fiber tube for human cancer tissue transplantation and its application. Background technique [0003] The treatment of human cancer is one of the worldwide problems in the field of medicine. In order to find a solution to this difficult problem, various new treatment methods such as antineoplastic drugs, radiotherapy, and gene therapy emerge in an endless stream, and a large number of preclinical studies have been carried out. The establishment of tumor experimental animal models provides a highly simulated tool for the study of its path...
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IPC IPC(8): C12N5/09A01K67/027
CPCA01K2227/105C12N5/0693A01K67/0271C12N2509/10C12N2509/00C12N2535/00A01K2207/12A01K2267/0331
Inventor 杨金波张斌
Owner 东部细胞治疗(山东)有限公司