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Building method of human cancer tissue transplantation tumor hollow fiber testing method mouse model

A technology of mouse model and method establishment, applied in the medical field, can solve the problem of lack of tumor molecular markers in the screening and verification platform, and achieve the effect of reducing the dosage and shortening the experimental cycle

Inactive Publication Date: 2020-01-10
东部细胞治疗(山东)有限公司
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AI Technical Summary

Problems solved by technology

At present, the lack of rapid and effective screening and verification platform is the bottleneck of tumor molecular marker research

Method used

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  • Building method of human cancer tissue transplantation tumor hollow fiber testing method mouse model
  • Building method of human cancer tissue transplantation tumor hollow fiber testing method mouse model
  • Building method of human cancer tissue transplantation tumor hollow fiber testing method mouse model

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Embodiment Construction

[0029] For ease of understanding, the technical solutions of the present invention are further specifically described below in conjunction with the accompanying drawings and through embodiments:

[0030] 1. Experimental materials

[0031] 1.1. Experimental animals

[0032] Nude mice of Balb / CSPF grade, female, 6-8 weeks old, were purchased from Shanghai Slack Experimental Animal Co., Ltd., production license number: SCXK (Shanghai) 2007-(8)05.

[0033] 1.2. Reagents

[0034] RPM-1640 medium: Gino Biomedical Technology Co., Ltd.;

[0035] Fetal bovine serum: Gibco, USA.

[0036] 1.3. Instruments

[0037] 37°CCO 2 Constant temperature incubator: BINDER, Germany;

[0038] Heal Force ultra-clean workbench: Likang Biomedical Technology Holdings Co., Ltd.;

[0039] Inverted phase contrast microscope: ZEISS, Germany;

[0040] -80℃ ultra-low temperature refrigerator: Haier Company;

[0041] PVDF tube with a molecular cut-off of 58kDa and an inner diameter of 1.0mm (Spectrum Med...

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Abstract

The invention relates to a building method of a human cancer tissue transplantation tumor hollow fiber testing method mouse model. The building method includes steps: 1), clinically screening; 2), performing tissue treatment; 3), performing experimental animal preparation; 4), performing hollow fiber tube pretreatment; 5), performing tissue pretreatment; 6), performing cell filling; 7), performingmouse transplantation. According to the method, the human cancer tissue hollow fiber testing method model is built by filling tumor tissue after a cancer patient receives an operation into a hollow fiber tube and transplanting the hollow fiber tube into an immunodeficiency mouse to enable tumor formation of the mouse for the convenience of detecting drug treatment effect. The method effectively solves the methodology problem of anticancer drug in-vivo screening and has the advantages of being high throughput (various tumor cells and tumor tissue can be screened in one time), short in experimental period, low in animal and tested drug consumption and capable of providing vivid in-vivo environment, and a precise evaluation platform can be provided for antitumor drug screening and personalized treatment of tumor and cancer clinically.

Description

technical field [0001] The invention relates to the medical field, and relates to a method for establishing an animal model, in particular to a method for establishing a mouse model of a human cancer tissue transplanted tumor hollow fiber test method. Background technique [0002] The treatment of human cancer is one of the worldwide problems in the field of medicine. In order to find a solution to this difficult problem, various new treatment methods such as antineoplastic drugs, radiotherapy, and gene therapy emerge in an endless stream, and a large number of preclinical studies have been carried out. The establishment of tumor experimental animal models provides a highly simulated tool for the study of its pathogenesis, biological characteristics and detection of various therapeutic methods. Based on the ethical aspects and practical application considerations of human trials, accurate and fast animal models are crucial for the development of tumor drugs. Pharmaceutical...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/027A61D1/00A61D7/00
CPCA01K2227/105C12N5/0693A01K67/0271C12N2509/10C12N2509/00C12N2535/00A01K2207/12A01K2267/0331
Inventor 杨金波张斌
Owner 东部细胞治疗(山东)有限公司