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Composition of subunit dengue vaccine

A composition and unit technology, applied in the field of recombinant dengue subunit vaccine composition, can solve problems such as increasing DENV infection

Pending Publication Date: 2022-01-04
林以行
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, antibodies against E protein can not only neutralize DENV, but may also increase DENV infection through antibody-dependent enhancement (ADE) (Guzman et al., 2015)

Method used

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  • Composition of subunit dengue vaccine
  • Composition of subunit dengue vaccine
  • Composition of subunit dengue vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0056] Create an experimental animal model

[0057]Strain C3H / HeN mice were obtained from Charles River Breeding Laboratories. Mice were fed with standard laboratory-grade feed and water in the Experimental Animal Center of Chenggong University School of Medicine. Mice aged 6 to 8 weeks or offspring aged 3 weeks were used for antibody production and infection experiments. The above animal experiment protocol has been reviewed and approved by the Institutional Animal Care and Use Committee (IACUC). The following examples have been approved by the Animal Experiment Ethics Committee of Chenggong University.

[0058] Preparation of recombinant protein and serum

[0059] This example is to construct the cEDIII-NS1ΔC polypeptide, which is a construct of fusion DNA fragments of NS1ΔC and cEDIII (provided by Dr. Chen Xinwei), and inserted into the pET21b plastid with restriction enzymes Not I and Xho I sites. The C-terminus of the resulting recombinant protein has additional 6 con...

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Abstract

The present invention relates to a composition of a subunit dengue vaccine comprising a fusion protein of conjugating or connecting delta C nonstructural protein 1 (NS1[delta]C or truncated NS1[delta]C) to at least one polypeptides of NS3c (or truncated NS3c) and / or consensus envelope protein domain III (cEDIII), thereby enhancing better protection against DENV challenge and alleviating associated pathological effects.

Description

technical field [0001] The present invention relates to a subunit vaccine composition, in particular to a non-structural protein 1 (NS1ΔC or truncated NS1ΔC) containing C-terminal removed, conjugated or linked to non-structural protein 3c (NS3c or truncated NS3c) and / or A recombinant dengue subunit vaccine composition of a fusion protein of at least one polypeptide of the third block of the homologous envelope protein (cEDIII), wherein at least one polypeptide is non-structural protein 3c (NS3c or truncated NS3c) and / or Homologous envelope protein block III (cEDIII). Background technique [0002] Dengue virus (DENV) is the most important arbovirus disease in global public health (WHO, 2016). DENV infects humans via Aedes aegypti and Aedes albopictus mosquitoes, causing an estimated 390 million cases of infection each year, of which 96 million cases have overt symptoms and 500,000 cases have dengue hemorrhagic fever Or dengue shock syndrome (dengue hemorrhagic fever / dengue ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/12A61P31/14C07K19/00
CPCA61K39/12A61P31/14C07K14/005C12N2770/24122C07K2319/00C12N2770/24134A61K2039/55505A61K2039/575A61K2039/70Y02A50/30C12N7/00C12N2770/24131
Inventor 林以行叶才明庄詠钧余佳益陈信伟万书彣王淑莺何宗宪谢达斌
Owner 林以行
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